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Effect of photobiomodulation on mitochondrial dynamics in peripheral nervous system in streptozotocin-induced type 1 diabetes in rats

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Abstract

There is no effective treatment to halt peripheral nervous system damage in diabetic peripheral neuropathy. Mitochondria have been at the center of discussions as important factors in the development of neuropathy in diabetes. Photobiomodulation has been gaining clinical acceptance as it shows beneficial effects on a variety of nervous system disorders. In this study, the effects of photobiomodulation (904 nm, 45 mW, 6.23 J/cm2, 0.13 cm2, 60 ns pulsed time) on mitochondrial dynamics were evaluated in an adult male rat experimental model of streptozotocin-induced type 1 diabetes. Results presented here indicate that photobiomodulation could have an important role in preventing or reversing mitochondrial dynamics dysfunction in the course of peripheral nervous system damage in diabetic peripheral neuropathy. Photobiomodulation showed its effects on modulating the protein expression of mitofusin 2 and dynamin-related protein 1 in the sciatic nerve and in the dorsal root ganglia neurons of streptozotocin-induced type 1 diabetes in rats.

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Abbreviations

CTRL:

Control

DRP-1:

Dynamin-related protein 1

DRG:

Dorsal root ganglia

i.p:

Intraperitoneal

MFN-2:

Mitofusin-2

PBM:

Photobiomodulation

SN:

Sciatic nerve

STZ:

Streptozotocin

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Acknowledgements

Funding for this work was generously provided by Sao Paulo Research Foundation—FAPESP (grant number 2017/25,554–0; 2017/05,218–5).

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Authors and Affiliations

  1. Department of Anatomy, Biomedical Sciences Institute, University of Sao Paulo, Avenue Lineu Prestes 2415, Room 007, Sao Paulo, SP, 05508-900, Brazil

    Igor Rafael Correia Rocha & Marucia Chacur

  2. Department of Pharmacology, School of Medicine, University of Virginia, Charlottesville, VA, USA

    Edward Perez-Reyes

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  1. Igor Rafael Correia Rocha
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  2. Edward Perez-Reyes
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Correspondence to Marucia Chacur.

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The authors declare that they have no conflict of interest.

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All procedures of the study were conducted in accordance with the ethical guidelines set by Institutional Animal Care Committee of the University of Sao Paulo. Protocol number 123/2015. The funding agency plays no role in the design of the study, data collection, analysis, interpretation of the data, or in writing the manuscript.

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Rocha, I.R.C., Perez-Reyes, E. & Chacur, M. Effect of photobiomodulation on mitochondrial dynamics in peripheral nervous system in streptozotocin-induced type 1 diabetes in rats. Photochem Photobiol Sci 20, 293–301 (2021). https://doi.org/10.1007/s43630-021-00018-w

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