Abstract
Artificial colours are extensively used in various industries like food, textiles, cosmetics and pharmaceuticals. Synthetic pigments pose toxicity threats and this has provoked an interest in the field of microbial colours and dyes. Pigment producing microorganisms exhibit a promising potential in overcoming current challenges. These pigments are biodegradable and eco-friendly. At present, natural pigments, especially microbial pigments hold great significance within the market. Numerous microorganisms produce colored pigments. This review focuses on the antimicrobial and antifungal activity of pyocyanin, pigment from Pseudomonas aeruginosa. It may find application in preparation of sheets and gown for medical and hospital use. Various fermentation techniques are discussed in view of their production by some important microorganisms. Further, it also sheds light on the possibility of using this pigment as textile colourant or dye which can be used to colour cotton, wool and silk fabrics.
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References
Abdul-Hussein, Z.R., Atia, S.S.: Antimicrobial effect of pyocyanin extracted from Pseudomonas aeruginosa. Eur. J. Exp. Biol. 6(6), 1–4 (2016)
Abdulkadir, N.: Bacterial pigments and its significance. MOJ Bioequiv. Bioavailab. (2017). https://doi.org/10.15406/mojbb.2017.04.00073
Ahmad, W.A., et al.: Production and characterization of violacein by locally isolated Chromobacterium violaceum grown in agricultural wastes. Appl. Biochem. Biotechnol. 167(5), 1220–1234 (2012). https://doi.org/10.1007/s12010-012-9553-7
Alzahrani, S.H., Alqahtani, F.S.: Pyocyanin pigment extracted from Pseudomonas aeruginosa isolate as antimicrobial agent and textile colourant. Int. J. Sci. Res. (2016). https://doi.org/10.36106/ijsr
Araújo, D., et al.: The role of ecological constraints on expertise development. Talent Dev. Excell. 2(2), 165–179 (2010)
Aslim, D., Onbasli, B.: Determination of antimicrobial activity and production of some metabolites by Pseudomonas aeruginosa B1 and B2 in sugar beet molasses. Afr. J. Biotechnol. 7(24), 4614 (2008)
Audenaert, K., et al.: Induction of Systemic Resistance to Botrytis cinerea in Tomato by Pseudomonas aeruginosa 7NSK2: role of salicylic acid, pyochelin, and pyocyanin. Mol. Plant-Microbe Interact. MPMI 15(11), 1147–1156 (2002). https://doi.org/10.1094/mpmi.2002.15.11.1147
Ayuningrum, D., et al.: “Isolation, characterisation and antagonistic activity of bacteria symbionts hardcoral Pavona sp. Isolated from panjang island, jepara against infectious multi-drug resistant (MDR) bacteria. IOP Conf. Ser. Earth Environ. Sci. 55, 012029 (2017). https://doi.org/10.1088/1755-1315/55/1/012029
Azlina, A.W., et al.: Application of Bacterial Pigments as Colorant. Springer, Berlin (2012)
Barakat, K.M., et al.: Production and characterization of bioactive pyocyanin pigment by marine Pseudomonas aeruginosa OSh1. Res. J. Pharm. Biol. Chem. Sci. 6(5), 933–943 (2015)
Baron, S.S., Rowe, J.J.: Antibiotic action of pyocyanin. Antimicrob. Agents Chemother. 20(6), 814–820 (1981)
Boonyapranai, K. et al.: Optimization of submerged culture for the production of naphthoquinones pigment by Fusarium verticillioides. https://www.semanticscholar.org/paper/c36565538b7d834533e478dacb4edbbe3632ca3e (2008). Accessed 21 Mar 2022
Bouhet, S., Oswald, I.P.: The effects of mycotoxins, fungal food contaminants, on the intestinal epithelial cell-derived innate immune response. Vet. Immunol. Immunopathol. 108(1–2), 199–209 (2005). https://doi.org/10.1016/j.vetimm.2005.08.010
Castañeda-Tamez, P., et al.: Pyocyanin restricts social cheating in Pseudomonas aeruginosa. Front. Microbiol. (2018). https://doi.org/10.3389/fmicb.2018.01348
Chadni, Z., et al.: Extraction and optimisation of red pigment production as secondary metabolites from Talaromyces verruculosus and its potential use in textile industries. Mycology 8(1), 48–57 (2017). https://doi.org/10.1080/21501203.2017.1302013
Cheluvappa, R., et al.: Reactions of Pseudomonas aeruginosa pyocyanin with reduced glutathione. Acta Biochim. Pol. 55(3), 571–580 (2008). https://doi.org/10.18388/abp.2008_3063
Chintapenta, L.K., et al.: Pigment production from a mangrove Penicillium. Afr. J. Biotechnol. 13(26), 2668–2674 (2014). https://doi.org/10.5897/ajb2014.13838
Cho, Y.J., et al.: Production of red pigment by submerged culture of Paecilomyces sinclairii. Lett. Appl. Microbiol. 35(3), 195–202 (2002). https://doi.org/10.1046/j.1472-765x.2002.01168.x
DeBritto, et al.: Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties. Sci. Rep. 10(1), 1542 (2020). https://doi.org/10.1038/s41598-020-58335-6
de Morais, O.B., et al.: Craft beer waste as substrate for pyocyanin synthesis. IOSR J. Pharm. Biol. Sci. 14(1), 21–25 (2019). https://doi.org/10.9790/3008-1401042125
Devnath, P., et al.: Extraction, purification and characterization of pyocyanin produced by Pseudomonas aeruginosa and evaluation for its antimicrobial activity. Int. Res. J. Biol. Sci. 6(5), 1–9 (2017)
Dhale, M. A.: Physiology of Monascus purpureus in Relation to Metabolite Production and Application as Functional Food. University of Mysore (2007)
Dharni, S., et al.: Production, purification, and characterization of antifungal metabolite from Pseudomonas aeruginosa SD12, a new strain obtained from tannery waste polluted soil. J. Microbiol. Biotechnol. 22(5), 674–683 (2012). https://doi.org/10.4014/jmb.1109.09061
El Feghali Patrick, R., Nawas, T.: Extraction and purification of pyocyanin: a simpler and more reliable method. MOJ Toxicol. (2018). https://doi.org/10.15406/mojt.2018.04.00139
El-Fouly, M.Z., et al.: Biosynthesis of pyocyanin pigment by Pseudomonas aeruginosa. J. Radiat. Res. Appl. Sci. 8(1), 36–48 (2015). https://doi.org/10.1016/j.jrras.2014.10.007
Fontoura, R., et al.: Purification and characterization of an antimicrobial peptide produced by Pseudomonas sp. strain 4B. World J. Microbiol. Biotechnol. 25(2), 205–213 (2009). https://doi.org/10.1007/s11274-008-9882-4
Frank, L.H., DeMoss, R.D.: On the biosynthesis of pyocyanin 1. J. Bacteriol. 77(6), 776–782 (1959). https://doi.org/10.1128/jb.77.6.776-782.1959
Fried, R., et al.: Biogenic colourants in the textile industry—a promising and sustainable alternative to synthetic dyes. Green Chem. Int. J. Green Chem. Resour. GC 24(1), 13–35 (2022). https://doi.org/10.1039/d1gc02968a
Gahlout, M., et al.: Characterization, application and statistical optimization approach for enhanced production of pyocyanin pigment by Pseudomonas aeruginosa DN9. Syst. Microbiol. Biomanuf. 1(4), 459–470 (2021). https://doi.org/10.1007/s43393-021-00033-z
Grossart, H.-P., et al.: Production of a blue pigment (glaukothalin) by marine Rheinheimera spp. Int. J. Microbiol. 2009, 701735 (2009). https://doi.org/10.1155/2009/701735
Gunasekaran, A., Lai, K., Edwincheng, T.: Responsive supply chain: a competitive strategy in a networked economy. Omega 36(4), 549–564 (2008). https://doi.org/10.1016/j.omega.2006.12.002
Gupta, R.: Veterinary Toxicology: Basic and Clinical Principles, 3rd edn. Academic Press, San Diego, CA (2018)
Hall, S., et al.: Cellular effects of pyocyanin, a secreted virulence factor of Pseudomonas aeruginosa. Toxins 8(8), 236 (2016). https://doi.org/10.3390/toxins8080236
Hamad, M.N.F., Marrez, D.A., El-Sherbieny, S.M.R.: Toxicity evaluation and antimicrobial activity of purified pyocyanin from Pseudomonas aeruginosa. Biointerface Res. Appl. Chem. 10(6), 6974–6990 (2020). https://doi.org/10.33263/briac106.69746990
Hashmatullah, Y., Zia, N. A.: Strategies for enhancement of microbial secondary metabolites. Res. Rev. Int. J. Multidiscip. 4(7) (2019)
Hassett, D.J., et al.: Response of Pseudomonas aeruginosa to pyocyanin: mechanisms of resistance, antioxidant defences, and demonstration of a manganese-cofactored superoxide dismutase. Infect. Immun. 60(2), 328–336 (1992). https://doi.org/10.1128/iai.60.2.328-336.1992
Heer, K., Sharma, S.: Microbial pigments as a natural color: a review. Int. J. Pharm. Sci. Res. 8, 1913–1922 (2017)
Hizbullah, M.U., et al.: Studies on bio-color production by Pseudomonas aeruginosa isolated from soil. J. Adv. Microbiol. 12(1), 1–12 (2018). https://doi.org/10.9734/jamb/2018/43068
Jameel, Z.J., et al.: Bioactivity of pyocyanin of Pseudomonas aeruginosa clinical isolates against a variety of human pathogenic bacteria and fungi species. Int. Arabic J. Antimicrob. Agents (2017). https://doi.org/10.3823/0812
Jayaseelan, S., Ramaswamy, D., Dharmaraj, S.: Pyocyanin: production, applications, challenges and new insights. World J. Microbiol. Biotechnol. 30(4), 1159–1168 (2014). https://doi.org/10.1007/s11274-013-1552-5
Jingjing, J., et al.: Detection of high-affinity and sliding clamp modes for MSH2-MSH6 by single-molecule unzipping force analysis. Mol. Cell 20(5), 771–781 (2005). https://doi.org/10.1016/j.molcel.2005.10.014
Johnson, E.A., Lewis, M.J.: Astaxanthin formation by the yeast Phaffia rhodozyma. J. Gen. Microbiol. 115(1), 173–183 (1979). https://doi.org/10.1099/00221287-115-1-173
Joshi, V. K. et al.: Microbial Pigments, NISCAIR-CSIR, India, pp. 362–369. http://hdl.handle.net/123456789/11334 (2003)
Kaleli, I., et al.: Anticandidal activity of Pseudomonas aeruginosa strains isolated from clinical specimens. Mycoses 50(1), 74–78 (2007). https://doi.org/10.1111/j.1439-0507.2006.01322.x
Kerr, J.: Inhibition of fungal growth by Pseudomonas aeruginosa and Pseudomonas cepacia isolated from patients with cystic fibrosis. J. Infect. 28(3), 305–310 (1994). https://doi.org/10.1016/s0163-4453(94)91943-7
Kerr, J.R., et al.: Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth. J. Clin. Pathol. 52(5), 385–387 (1999). https://doi.org/10.1136/jcp.52.5.385
Khoury, A.E., et al.: Prevention and control of bacterial infections associated with medical devices. ASAIO J. Am. Soc. Artif. Intern. Organs 38(3), M174–M178 (1992). https://doi.org/10.1097/00002480-199207000-00013
King, E.O., Ward, M.K., Raney, D.E.: Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44(2), 301–307 (1954)
Korumilli, T.: Studies on Pigment Production by Microorganisms Using Raw Materials of Agro-industrial Origin. National Institute of Technology, Rourkela (2014)
Krieg, N.R., Brenner, D.J., Staley, J.R. (eds.): Bergey’s Manual (R) of Systematic Bacteriology: Volume 2: The Proteobacteria, Part B: The Gammaproteobacteria, 2nd edn. Springer, New York, NY (2005)
Krishna, J. G. et al.: Marine bacteria as source of pigment for application as dye in textile industry. In: International Conference on Biodiversity Conservation and Management. https://www.file:///C:/Users/user/Downloads/11.JissaBIOCAM.pdf (2008)
Kulandaisamy, V.C., Renuka, D.P., Azlina, A.W.: Agro-industrial waste as substrates for the production of bacterial pigment. In: Zakaria, Z.A., Boopathy, R. (eds.) Valorisation of agro-industrial residues—volume i: biological approaches, pp. 149–162. Springer, Cham (2020)
Kumar, R.S., et al.: Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits. J. Appl. Microbiol. 98(1), 145–154 (2005). https://doi.org/10.1111/j.1365-2672.2004.02435.x
Kumar, A., et al.: Microbial pigments: production and their applications in various industries. Int. J. Pharm. Chem. Biol. 5(1), 203–212 (2015)
Kunihiko, O., et al.: Construction of a glucose sensor based on a screen-printed electrode and a novel mediator pyocyanin from Pseudomonas aeruginosa. Biosens. Bioelectron. 19(10), 1237–1244 (2004). https://doi.org/10.1016/j.bios.2003.11.010
Lundgren, B.R., et al.: Gene PA2449 is essential for glycine metabolism and pyocyanin biosynthesis in Pseudomonas aeruginosa PAO1. J. Bacteriol. 195(9), 2087–2100 (2013). https://doi.org/10.1128/JB.02205-12
Mahmoud, S.Y., et al.: Antifungal activity of pyocyanin produced by Pseudomonas aeruginosa against Fusarium oxysporum Schlech a root-rot phytopathogenic fungi. Int. J. Pharmtech Res. 9(8), 43–50 (2016)
Malik, K., Tokka, J., Goyal, S.: Microbial pigments: a review. Int. J. Microb. Resour. Technol. 1(4), 361 (2012)
Mavrodi, D.V., et al.: Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1. J. Bacteriol. 183(21), 6454–6465 (2001). https://doi.org/10.1128/JB.183.21.6454-6465.2001
Mishra, A., Jahan, S.: A novel natural dye from Pseudomonas fluorescens imparts antibacterial finish and ultraviolet radiation resistance to textiles. Asian J. Home Sci. 13(1), 321–327 (2018). https://doi.org/10.15740/has/ajhs/13.1/321-327
Morales, D.K., et al.: Control of Candida albicans metabolism and biofilm formation by Pseudomonas aeruginosa phenazines. Mbio 4(1), e00526–e00612 (2013). https://doi.org/10.1128/mBio.00526-12
Murugesh Babu, K., Ravindra, K.B.: Bioactive antimicrobial agents for finishing of textiles for health care products. J. Text. Inst. 106(7), 706–717 (2015). https://doi.org/10.1080/00405000.2014.936670
Nagia, F.A., EL-Mohamedy, R.S.R.: Dyeing of wool with natural anthraquinone dyes from Fusarium oxysporum. Dyes Pigments Int. J. 75(3), 550–555 (2007). https://doi.org/10.1016/j.dyepig.2006.07.002
Narsing Rao, M.P., Xiao, M., Li, W.-J.: Fungal and bacterial pigments: secondary metabolites with wide applications. Front. Microbiol. 8, 1113 (2017). https://doi.org/10.3389/fmicb.2017.01113
Nigam, P. S. N., Pandey, A.: Solid-state fermentation technology for bioconversion of biomass and agricultural residues. In: Solid State Fermentation for the Production of Industrial Enzymes. Current Science (1999)
Norman, R.S., et al.: Effect of pyocyanin on a crude-oil-degrading microbial community. Appl. Environ. Microbiol. 70(7), 4004–4011 (2004). https://doi.org/10.1128/AEM.70.7.4004-4011.2004
Özyürek, S.B., Gür, S.D., Bilkay, I.S.: Investigation of antimicrobial activity of pyocyanin produced by Pseudomonas aeruginosa strains isolated from different clinical specimens. Hacettepe J. Biol. Chem. 44(1), 1–6 (2016). https://doi.org/10.15671/HJBC.20164417526
Pachori, P., Gothalwal, R., Gandhi, P.: Emergence of antibiotic resistance Pseudomonas aeruginosa in intensive care unit; a critical review. Genes Dis. 6(2), 109–119 (2019). https://doi.org/10.1016/j.gendis.2019.04.001
Parr, L. W.: “Bergey’s Manual of Determinative Bacteriology. Robert S. Breed, E. G. D. Murray, and Nathan R. Smith. Williams & Wilkins, Baltimore, ed. 7, 1957. xviii+ 1094 pp. $15. Science (New York, N.Y.) 127(3305), 1043–1044 (1958). https://doi.org/10.1126/science.127.3305.1043.b
Pham, J.V., et al.: A review of the microbial production of bioactive natural products and biologics. Front. Microbiol. 10, 1404 (2019). https://doi.org/10.3389/fmicb.2019.01404
Przystaś, W., Zabłocka-Godlewska, E., Grabińska-Sota, E.: Biological removal of azo and triphenylmethane dyes and toxicity of process by-products. Water Air Soil Pollut. 223(4), 1581–1592 (2012). https://doi.org/10.1007/s11270-011-0966-7
Rahman, P.K.S.M., et al.: Development of a simple and low cost microbioreactor for high-throughput bioprocessing. Biotechnol. Lett. 31(2), 209–214 (2009). https://doi.org/10.1007/s10529-008-9853-8
Ramesh, C., et al.: Multifaceted applications of microbial pigments: current knowledge, challenges and future directions for public health implications. Microorganisms 7(7), 186 (2019). https://doi.org/10.3390/microorganisms7070186
Ratna, Padhi, B. S.: Pollution due to synthetic dyes toxicity & carcinogenicity studies and remediation. Int. J. Environ. Sci. (2012). https://doi.org/10.6088/ijes.2012030133002
Reszka, K.J., et al.: Oxidation of pyocyanin, a cytotoxic product from Pseudomonas aeruginosa, by microperoxidase 11 and hydrogen peroxide. Free Radical Biol. Med. 36(11), 1448–1459 (2004). https://doi.org/10.1016/j.freeradbiomed.2004.03.011
Rodríguez, C.S., Moldes, D., Sanromán, M.A.: Optimum stability conditions of pH and temperature for ligninase and manganese-dependent peroxidase from Phanerochaete chrysosporium. Application to in vitro decolorization of Poly R-478 by MnP. World J. Microbiol. Biotechnol. 22(6), 607–612 (2006). https://doi.org/10.1007/s11274-005-9078-0
Samanta, S., Thavasi, R., Jayalakshmi, S.: Phenazine pigments from Pseudomonas aeruginosa and their application as antibacterial agent and food colourants. Res. J. Microbiol. 3(3), 122–128 (2008). https://doi.org/10.3923/jm.2008.122.128
Sanjay, K.R., et al.: Optimization of carotenoid production by Aspergillus carbonarius in submerged fermentation using a response surface methodology. Int. J. Food Eng. (2007). https://doi.org/10.2202/1556-3758.1295
Sarvamangala, D., Aparna, S.S.V.: Microbial pigments-a short review. IOSR J. Environ. Sci. Toxicol. Food Technol. 10(8), 1–7 (2016)
Schalk, I.J., Rigouin, C., Godet, J.: An overview of siderophore biosynthesis among fluorescent Pseudomonads and new insights into their complex cellular organization. Environ. Microbiol. 22(4), 1447–1466 (2020). https://doi.org/10.1111/1462-2920.14937
Sheet, S., et al.: Highly flexible electrospun hybrid (polyurethane/dextran/pyocyanin) membrane for antibacterial activity via generation of oxidative stress. ACS Omega 3(11), 14551–14561 (2018). https://doi.org/10.1021/acsomega.8b01607
Shirata, A., et al.: Isolation of bacteria producing bluish-purple pigment and use for dyeing. Jpn. Int. Res. Center Agric. Sci. 34(2), 131–140 (2000)
Sterritt, O.W., et al.: Structural and functional characterisation of the entry point to pyocyanin biosynthesis in Pseudomonas aeruginosa defines a new 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase subclass. Biosci. Rep. 38(5), BSR20181605 (2018). https://doi.org/10.1042/bsr20181605
Sudha, Gupta, C., Aggarwal, S.: Dyeing wet blue goat nappa skin with a microbial colorant obtained from Penicillium minioluteum. J. Clean. Prod. 127, 585–590 (2016). https://doi.org/10.1016/j.jclepro.2016.03.043
Sudhakar, T., Karpagam, S., Premkumar, J.: Biosynthesis, antibacterial activity of pyocyanin pigment produced by Pseudomonas aeruginosa SU1. J. Chem. Pharm. Res. 7(3), 921–924 (2015)
Sweedan, E.G.: Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria. J. Univ. Anbar Pure Sci. 4(1), 15 (2010)
Tarangini, K., Mishra, S.: Carotenoid production by Rhodotorula sp. On fruit waste extract as a sole carbon source and optimization of key parameters. Iran. J. Chem. Chem. Eng. (1990) 33(3), 89–99 (2014). https://doi.org/10.30492/ijcce.2014.11344
Thiago, G., Ulrich, V.: Colour me blue: the history and the biotechnological potential of pyocyanin. Molecules (basel, Switzerland) 26(4), 927 (2021). https://doi.org/10.3390/molecules26040927
Tinoi, J., Rakariyatham, N., Deming, R.L.: Simplex optimization of carotenoid production by Rhodotorula glutinis using hydrolyzed mung bean waste flour as substrate. Process Biochem. (barking, London, England) 40(7), 2551–2557 (2005). https://doi.org/10.1016/j.procbio.2004.11.005
Tuli, H.S., et al.: Microbial pigments as natural colour sources: current trends and future perspectives. J. Food Sci. Technol. 52(8), 4669–4678 (2015). https://doi.org/10.1007/s13197-014-1601-6
Vasanthabharathi, V., Lakshminarayanan, R., Jayalakshmi, S.: Melanin production from marine Streptomyces. Afr. J. Biotechnol. 10(54), 11224–11234 (2011). https://doi.org/10.5897/ajb11.296
Velmurugan, P., et al.: Natural pigment extraction from five filamentous fungi for industrial applications and dyeing of leather. Carbohydr. Polym. 79(2), 262–268 (2009)
Venil, C.K., et al.: Current perspective on bacterial pigments: emerging sustainable compounds with coloring and biological properties for the industry—an incisive evaluation. RSC Adv. (2014). https://doi.org/10.1039/C4RA06162D
Weber, E.J., Adams, R.L.: Chemical- and sediment-mediated reduction of the azo dye disperse blue 79. Environ. Sci. Technol. 29(5), 1163–1170 (1995). https://doi.org/10.1021/es00005a005
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Srivastava, P., Ramesh, M., Kaushik, P. et al. Pyocyanin pigment from Pseudomonas species: Source of a dye and antimicrobial textile finish—a review. Proc.Indian Natl. Sci. Acad. 88, 542–550 (2022). https://doi.org/10.1007/s43538-022-00109-x
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DOI: https://doi.org/10.1007/s43538-022-00109-x