Abstract
Background
The objective of our study was to measure and compare the elution characteristics of teicoplanin from poly(methyl methacrylate) PMMA beads with those of poly(glycolide-co-lactide) PGLA-added beads.
Methods
The study included two groups of PMMA + teicoplanin beads. PMMA was added to teicoplanin in Group 1 and PMMA + PGLA was added to teicoplanin in Group 2. A total of 16 beads of 1 cm3 were created for each group. Samples were added individually to tubes containing 3 ml of phosphate-buffered saline (PBS). Antibiotic elution was measured by measuring absorbance values of 1-ml samples taken at regular intervals using a UV–Vis spectrophotometer and cumulative percentages of drug release were calculated. In addition, the spectra of teicoplanin were identified using a FTIR spectrophotometer in a wavelength range of 400–4000 cm−1.
Results
Drug elution in the PBS medium was measured and compared for Groups 1 and 2. The cumulative percentage of drug release from the PGLA-added beads (Group 2) was significantly higher (p = 0.01). The molecular structure of teicoplanin was also confirmed using FTIR.
Conclusion
The in vitro results showed that the addition of biodegradable PGLA into bone cement functions as a water-soluble porogen which allows for significant increases in the elution of teicoplanin from cement. This increase in elution suggests that the PGLA would allow for further fluid contact and exchange with the previously entrapped drug. These results may have important clinical applications.
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References
Widmer, A. F. (2001). New developments in diagnosis and treatment of ınfection in orthopedic ımplants. Clinical Infectious Diseases, 33(Suppl 2), S94–106.
Trampuz, A., & Widmer, A. F. (2006). Infections associated with orthopedic implants. Current Opinion in Infectious Diseases, 19(4), 349–356.
Bistolfi, A., Massazza, G., Verné, E., Massè, A., Deledda, D., Ferraris, S., et al. (2011). Antibiotic-loaded cement in orthopedic surgery: A review. ISRN Orthopedics, 7(2011), 290851.
Garvin, K. L., & Hanssen, A. D. (1995). Infection after total hip arthroplasty. Past, present, and future. Journal of Bone and Joint Surgery, 77(10), 1576–1588.
van Vugt, T. A. G., Arts, J. J., & Geurts, J. A. P. (2019). Antibiotic-loaded polymethylmethacrylate beads and spacers in treatment of orthopedic infections and the role of biofilm formation. Frontiers in Microbiology, 25(10), 1626.
Atıcı, T., Şahin, N., Çavun, S., Özakin, C., & Kaleli, T. (2018). Antibiotic release and antibacterial efficacy in cement spacers and cement beads impregnated with different techniques: In vitro study. Eklem Hastalik Cerrahisi, 29(2), 71–78.
Bertazzoni Minelli, E., Caveiari, C., & Benini, A. (2002). Release of antibiotics from polymethylmethacrylate cement. Journal of Chemotherapy, 14(5), 492–500.
Drago, L., De Vecchi, E., Fassina, M. C., & Gismondo, M. R. (1998). Serum and bone concentrations of teicoplanin and vancomycin: Study in an animal model. Drugs Under Experimental and Clinical Research, 24(4), 185–190.
Funk, G. A., Burkes, J. C., Cole, K. A., Rahaman, M. N., & McIff, T. E. (2018). Antibiotic elution and mechanical strength of PMMA bone cement loaded with borate bioactive glass. Journal of Bone and Joint Infection, 3(4), 187–196.
Bala, I., Hariharan, S., & Kumar, M. N. (2004). PLGA nanoparticles in drug delivery: The state of the art. Critical Reviews in Therapeutic Drug Carrier Systems, 21(5), 387–422.
Wilson, A. P. (2000). Clinical pharmacokinetics of teicoplanin. Clinical Pharmacokinetics, 39(3), 167–183.
Brogden, R. N., & Peters, D. H. (1995). Erratum to: Teicoplanin. A reappraisal of its antimicrobial activity, pharmacokinetic properties and therapeutic efficacy. Drugs, 49(1), 70.
Bezstarosti, H., Van Lieshout, E. M. M., Voskamp, L. W., Kortram, K., Obremskey, W., McNally, M. A., et al. (2019). Insights into treatment and outcome of fracture-related infection: A systematic literature review. Archives of Orthopaedic and Trauma Surgery, 139(1), 61–72.
Mader, J. T., Calhoun, J., & Cobos, J. (1997). In vitro evaluation of antibiotic diffusion from antibiotic-impregnated biodegradable beads and polymethylmethacrylate beads. Antimicrobial Agents and Chemotherapy, 41(2), 415–418.
Hendriks, J. G., van Horn, J. R., van der Mei, H. C., & Busscher, H. J. (2004). Backgrounds of antibiotic-loaded bone cement and prosthesis-related infection. Biomaterials, 25(3), 545–556.
Klemm, K. (1979). Gentamicin-PMMA-beads in treating bone and soft tissue infections (author’s transl). Zentralblatt fur Chirurgie, 104(14), 934–942.
Chang, Y., Chen, W. C., Hsieh, P. H., Chen, D. W., Lee, M. S., Shih, H. N., et al. (2011). In vitro activities of daptomycin-, vancomycin-, and teicoplanin-loaded polymethylmethacrylate against methicillin-susceptible, methicillin-resistant, and vancomycin-intermediate strains of Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 55(12), 5480–5484.
Jia, W. T., Zhang, X., Zhang, C. Q., Liu, X., Huang, W. H., Rahaman, M. N., et al. (2010). Elution characteristics of teicoplanin-loaded biodegradable borate glass/chitosan composite. International Journal of Pharmaceutics, 387(1–2), 184–186.
Jain, R. A. (2000). The manufacturing techniques of various drug loaded biodegradable poly(lactide-co-glycolide) (PLGA) devices. Biomaterials, 21(23), 2475–2490.
Bertoldi, C., Zaffe, D., & Consolo, U. (2008). Polylactide/polyglycolide copolymer in bone defect healing in humans. Biomaterials, 29(12), 1817–1823.
Penner, M. J., Masri, B. A., & Duncan, C. P. (1996). Elution characteristics of vancomycin and tobrarnycin combined in acrylic bone-cement. Journal of Arthroplasty, 11(8), 939–944.
Slane, J. A., Vivanco, J. F., Rose, W. E., Squire, M. W., & Ploeg, H. L. (2014). The influence of low concentrations of a water soluble poragen on the material properties, antibiotic release, and biofilm inhibition of an acrylic bone cement. Materials Science and Engineering C: Materials for Biological Applications, 42, 168–176.
Tunney, M. M., Brady, A. J., Buchanan, F., Newe, C., & Dunne, N. J. (2008). Incorporation of chitosan in acrylic bone cement: Effect on antibiotic release, bacterial biofilm formation and mechanical properties. Journal of Materials Science. Materials in Medicine, 19(4), 1609–1615.
Shen, S. C., Ng, W. K., Dong, Y. C., Ng, J., & Tan, R. B. (2016). Nanostructured material formulated acrylic bone cements with enhanced drug release. Materials Science and Engineering C: Materials for Biological Applications, 1(58), 233–241.
Drognitz, O., Thorn, D., Krüger, T., Gatermann, S. G., Iven, H., Bruch, H. P., et al. (2006). Release of vancomycin and teicoplanin from a plasticized and resorbable gelatin sponge: In vitro ınvestigation of a new antibiotic delivery system with glycopeptides. Infection, 34(1), 29–34.
Weiss, B. D., Weiss, E. C., Haggard, W. O., Evans, R. P., McLaren, S. G., & Smeltzer, M. S. (2009). Optimized elution of daptomycin from polymethylmethacrylate beads. Antimicrobial Agents and Chemotherapy, 53(1), 264–266.
Anagnostakos, K., & Meyer, C. (2017). Antibiotic elution from hip and knee acrylic bone cement spacers: A systematic review. BioMed Research International, 2017, 4657874.
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Kilinç, S., Pazarci, Ö., Keklikcioğlu Çakmak, N. et al. Does the Addition of Poly(glycolide-co-lactide) to Teicoplanin-Containing Poly(methyl methacrylate) Beads Change the Elution Characteristics?. JOIO 54 (Suppl 1), 71–75 (2020). https://doi.org/10.1007/s43465-020-00116-4
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DOI: https://doi.org/10.1007/s43465-020-00116-4