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The Effect of Different Exercise Modalities on Sertoli-germ Cells Metabolic Interactions in High-fat Diet-induced Obesity Rat Models: Implication on Glucose and Lactate Transport, Igf1, and Igf1R-dependent Pathways

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Abstract

The study aimed to uncover a unique aspect of obesity-related metabolic disorders in the testicles induced by a high-fat diet (HFD) and explored the potential mitigating effects of exercise modalities on male fertility. Thirty mature male Wistar rats were randomly assigned to control, HFD-sole, moderate-intensity exercise with HFD (HFD+MICT), high-intensity continuous exercise with HFD (HFD+HICT), and high-intensity interval exercise with HFD (HFD+HIIT) groups (n=6/group). Intracytoplasmic carbohydrate (ICC) storage, expression levels of GLUT-1, GLUT-3, MCT-4, Igf1, and Igf1R, and testicular lactate and lactate dehydrogenase (LDH) levels were assessed. ICC storage significantly decreased in HFD-sole rats, along with decreased mRNA and protein levels of GLUT-1, GLUT-3, MCT-4, Igf1, and Igf1R. The HFD-sole group exhibited a notable reduction in testicular lactate and LDH levels (p<0.05). Conversely, exercise, particularly HIIT, upregulated ICC storage, expression levels of GLUT-1, GLUT-3, MCT-4, Igf1, and Igf1R, and enhanced testicular lactate and LDH levels. These results confirm that exercise, especially HIIT, has the potential to mitigate the adverse effects of HFD-induced obesity on testicular metabolism and male fertility. The upregulation of metabolite transporters, LDH, lactate levels, Igf1, and Igf1R expression may contribute to maintaining metabolic interactions and improving the glucose/lactate conversion process. These findings underscore the potential benefits of exercise in preventing and managing obesity-related male fertility issues.

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Acknowledgments

The authors would like to acknowledge that this research constitutes a segment of the Ph.D. thesis, approved by the Research Deputy of Urmia University. Additionally, the authors express gratitude to the RASTA Specialized Research Institute (RSRI) for their assistance in the laboratory. Lastly, we extend our appreciation to the Faculties of Sport Sciences and Veterinary Medicine for their support.

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  1. Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran

    Aref Habibi Maleki, Javad Tolouei Azar & Asghar Tofighi

  2. Department of Basic Sciences, Division of Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, P.O.BOX: 1177, Urmia, Iran

    Mazdak Razi

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Contributions

Aref Habibi Maleki: Conceptualization, Data curation, Formal analysis, Investigation, Methodology Validation, Writing – original draft.

Javad Tolouei Azar: Conceptualization, Methodology, Formal analysis, Supervised the research, Writing – review & editing.

Mazdak Razi: Conceptualization, Formal analysis, Supervised the laboratory and statistical analyses, Writing – review & editing.

Asghar Tofighi: Conceptualization, Methodology, Review & editing.

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Correspondence to Javad Tolouei Azar.

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Maleki, A.H., Azar, J.T., Razi, M. et al. The Effect of Different Exercise Modalities on Sertoli-germ Cells Metabolic Interactions in High-fat Diet-induced Obesity Rat Models: Implication on Glucose and Lactate Transport, Igf1, and Igf1R-dependent Pathways. Reprod. Sci. (2024). https://doi.org/10.1007/s43032-024-01533-8

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