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MiR-182-5p, MiR-192-5p, and MiR-493-5p Constitute a Regulatory Network with CRISP3 in Seminal Plasma Fluid of Teratozoospermia Patients

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Abstract

Numerous evidences suggested that microRNAs (miRs) could play an active and significant role during spermatogenesis. Cysteine-rich secretory protein (CRISP3) has a role in inflammatory response and is extremely over-expressed in adolescents with varicocele seminal plasma and modified semen analysis. Nowadays, the miRs expression’s association with their target genes is well recognized. The aim of this study was evaluating the association of CRISP3 and four candidate miRs among teratozoospermia (TZ) infertile men. First, we have selected four miRs, miR-182-5p, miR-192-5p, miR-204-5p, and miR-493-5p bioinformatically. After that, RNA was extracted from semen samples of 21 TZ patients and 20 normozoospermia (Norm). Then, their expression levels were assessed using real-time polymerase chain reaction method. In the next step, we quantified the expression of two CRISP3 protein isoforms, targeted by these miRs, using western blotting. According to our results, up-regulation of miR-182-5p, miR-192-5p, and miR-493-5p was observed. MiR-182-5p, miR-192-5p, and miR-493-5p showed good AUC values which can be introduced as possible biomarkers of TZ. In addition, the expression level of the CRISP3 glycosylated (31 kDa) isoform was significantly lower in TZ patients than Norm ones. Notably, in TZ patients, there was a possibly positive correlation of glycosylated CRISP3 expression with normal sperm morphology. According to our results, CRISP3 protein can play a significant role in male infertility especially in maturation formation of spermatozoa. Also, deregulation of the studied miRs, miR-182-5p, miR-92-5p, and miR-493-5p, can suggest a regulatory network between these miRs and CRISP3 isoforms and suggest their regulatory roles in male infertility.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We would like to acknowledge Royan Institute Infertility Clinic (Tehran, Iran), Hazrat Zahra Infertility Clinic (Shahrekord, Iran), and Vice Chancellor for Research and Technology Affairs of Shahrekord University of Medical Sciences for supporting this study.

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Funding

This study has been supported financially by Royan Institute Infertility Clinic (Tehran, Iran) and Vice Chancellor for Research and Technology Affairs of Shahrekord University of Medical Sciences.

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Rahmatieh, Shahrekord, 8813833435, Iran

    Delnya Gholami, Tahereh Hasheminia & Hossein Teimori

  2. Genetics Division, Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran

    Farzane Amirmahani

  3. Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

    Reza Salman Yazdi

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  1. Delnya Gholami
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  2. Farzane Amirmahani
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  3. Reza Salman Yazdi
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  4. Tahereh Hasheminia
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  5. Hossein Teimori
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Contributions

All authors contributed to the study conception and design. Data collection and experimental works were performed by Delnya Gholami. The first draft of the manuscript was written by Farzane Amirmahani. Reza Salman Yazdi and Tahereh Hasheminia analyzed data and edited the drafted manuscript. Hossein Teimori contributed to study design and supervised the project. All authors read and approved the final manuscript.

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Correspondence to Hossein Teimori.

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Ethics Approval

All patients filled the informed consent form for participation. Ethical considerations were confirmed by the Shahrekord University of Medical Sciences ethical committee (ethics code: IR.SKUMS.REC.1395.214). The study was performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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A written informed consent was obtained from all individuals participated in this study.

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Gholami, D., Amirmahani, F., Yazdi, R.S. et al. MiR-182-5p, MiR-192-5p, and MiR-493-5p Constitute a Regulatory Network with CRISP3 in Seminal Plasma Fluid of Teratozoospermia Patients. Reprod. Sci. 28, 2060–2069 (2021). https://doi.org/10.1007/s43032-021-00485-7

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  • DOI: https://doi.org/10.1007/s43032-021-00485-7

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