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Differential expression of transport and signalling genes in leaves and panicle regulates the development of pollen-free anthers in TGMS red rice

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Abstract

Thermosensitive genic male sterile (TGMS) plants are male sterile above a critical sterility temperature (CST) and become male fertile below CST during a critical thermosensitive stage. It is essential to analyse the gene expression pattern under fertility- and sterility-inducing conditions to understand the mechanisms of male sterility since it is regulated by a single recessive nuclear gene sensitive to environment during a specific stage of panicle development. Hence, this study aims at understanding the molecular mechanism associated with pollen sterility in TGMS system. The newly developed TGMS line on red rice background exhibited male sterility with pollen-free anthers and hence can be recommended as a stable female parent for the development of suitable red rice hybrids to the state of Kerala. Microarray gene expression analysis of TGMS leaf and young panicle revealed that occurrence of pollen-free anthers in the TGMS line during sterility-inducing condition is mainly due to the down-regulation of genes encoding ABC transporter proteins, lipid transfer protein and strictosidine synthase, glucose-methanol-choline oxidoreductase, male sterility protein 2, wax synthase and β-1,3-glucanase. Moreover, pathways involved in the carbohydrate synthesis and transport were also down-regulated during sterility condition. The differential expression of transport and signalling genes regulates the development of pollen-free anthers in TGMS rice.

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The experimental material was developed by the second author through funding from Kerala Biotechnology commission.

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Acknowledgements

The authors thank the Associate Director of Research, Regional Agricultural Research Station (RARS), Ambalavayal, for permitting seed multiplication in the high altitude research station. Kerala Biotechnology commission is acknowledged for providing research grant to the second author through whom the experimental material was developed. The authors also thank Genotypic Technology Pvt Ltd., Bengaluru, for microarray gene expression analysis.

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  1. Department of Plant Physiology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, 695522, India

    R. Gayathri & Roy Stephen

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The first and the second author conceived and designed the research; the first author conducted the experiment, analysed the data and wrote the manuscript

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Communicated by J. Zimny.

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Gayathri, R., Stephen, R. Differential expression of transport and signalling genes in leaves and panicle regulates the development of pollen-free anthers in TGMS red rice. CEREAL RESEARCH COMMUNICATIONS 49, 465–473 (2021). https://doi.org/10.1007/s42976-020-00124-y

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