Abstract
Extreme environmental conditions like high salinity and temperature are continuously affecting fertile lands and trigger an ecological imbalance, thereby inducing indigenous microbial communities for adaptation to such extreme environments. Present study relates with the isolation and characterization of halo-thermotolerant bacteria (HTTB) from hyper-saline areas of districts Kashmore and Khairpur, Sindh, Pakistan. The isolated bacteria were initially screened for their tolerance to elevated salt concentrations and extreme temperature ranges. Out of twenty-six bacterial isolates, six bacteria from district Kashmore (60%) and thirteen bacterial isolates from district Khairpur (81.25%) tolerated NaCl concentration up to 15–17% (w/v), and grown efficiently when incubated in the temperature ranges 55–65 °C. The principle component and multivariate cluster analyses revealed three different clusters of HTTB bacteria based on their tolerance and spatial distribution. In general, the hyper-saline environments of district Kashmore harbored more HTTB than Khairpur, but individually there was extreme halotolerance and thermotolerance among Khairpur isolates. Finally, the PCR-based molecular typing of 16S rRNA genes revealed, majority of the isolates were identified to be various strains of genus Bacillus, i.e., Bacillus subtilis, B. licheniformis, B. aerius, B. vallismortis, B. aquimaris and B. flexus. Only one bacterial strain, i.e., KJ1WB, was identified as Pseudomonas pseudoalcaligenes. These HTTB bacterial strains may extend significant potential for future applications like environmental, biotechnological and enormous industrial due to their valuable enzymatic profiles and metabolite repositories.
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Asad, S., Amoozegar, M., Pourbabaee, A. A., Sarbolouki, M., & Dastgheib, S. (2007). Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresource Technology, 98, 2082–2088. https://doi.org/10.1016/j.biortech.2006.08.020
Baati, H., Amdouni, R., Gharsallah, N., Sghir, A., & Ammar, E. (2010). Isolation and characterization of moderately halophilic bacteria from Tunisian solar saltern. Current Microbiology, 60, 157–161. https://doi.org/10.1007/s00284-009-9516-6
Cheesbrough, M. (1991). Medical Laboratory manual for tropical countries: Microbiology (pp. 196–205). ELBS Edition, Cambridge.
Feng, C., Fang-yan, C., & Yu-bin, T. (2014). Isolation, identification of a halotolerant acid red B degrading strain and its decolorization performance. APCBEE Procedia, 9, 131–139. https://doi.org/10.1016/j.apcbee.2014.01.024
Kublanov, I. V., et al. (2009). Biodiversity of thermophilic prokaryotes with hydrolytic activities in hot springs of Uzon Caldera, Kamchatka (Russia). Applied and Environment Microbiology, 75, 286–291. https://doi.org/10.1128/AEM.00607-08
Kvist, T., Mengewein, A., Manzei, S., Ahring, B. K., & Westermann, P. (2005). Diversity of thermophilic and non-thermophilic crenarchaeota at 80 C. FEMS Microbiology Letters, 244, 61–68. https://doi.org/10.1016/j.femsle.2005.01.021
Margesin, R., & Schinner, F. (1999). Biological decontamination of oil spills in cold environments. Journal of Chemical Technology and Biotechnology, 74, 381–389. https://doi.org/10.1002/(SICI)1097-4660(199905)74:5%3c381::AID-JCTB59%3e3.0.CO;2-0
Mellado, E., & Ventosa, A. (2003). Biotechnological potential of moderately and extremely halophilic microorganisms. In J.-L. Barredo (Ed.), Microorganisms for health care, food and enzyme production (pp. 233–256). Research Signpost.
Menasria, T., et al. (2018). Diversity and bioprospecting of extremely halophilic archaea isolated from Algerian arid and semi-arid wetland ecosystems for halophilic-active hydrolytic enzymes. Microbiological Research, 207, 289–298. https://doi.org/10.1016/j.micres.2017.12.011
Müller, V., & Oren, A. (2003). Metabolism of chloride in halophilic prokaryotes. Extremophiles, 7, 261–266. https://doi.org/10.1007/s00792-003-0332-9
Oak, U., Ghattargi, V., Pawar, S., & Bhole, B. (2016). Degradation of Drimarene Red, a reactive textile dye by an extremophilic Bacillus sp. isolated from fresh water. International Journal of Applied and Pure Science, 2, 105–113.
Oren, A. (2002). Diversity of halophilic microorganisms: Environments, phylogeny, physiology, and applications. Journal of Industrial Microbiology and Biotechnology, 28, 56–63. https://doi.org/10.1038/sj/jim/7000176
Pakpitcharoen, A., Potivejkul, K., Kanjanavas, P., Areekit, S., & Chansiri, K. (2008). Biodiversity of thermotolerant Bacillus sp. producing biosurfactants, biocatalysts, and antimicrobial agents. Scientific Asia, 34, 424–431. https://doi.org/10.2306/scienceasia1513-1874.2008.34.424
Perez, S., Czerner, M., Patat, M. L., Zaritzky, N. E., Murialdo, S. E., & Yeannes, M. I. (2018). Monitoring the characteristics of cultivable halophilic microbial community during salted-ripened anchovy (Engraulis anchoita) production. International Journal of Food Microbiology, 286, 179–189. https://doi.org/10.1016/j.ijfoodmicro.2018.08.013
Phulpoto, A., Qazi, M., Mangi, S., Ahmed, S., & Kanhar, N. (2016). Biodegradation of oil-based paint by Bacillus species monocultures isolated from the paint warehouses. International Journal of Environmental Science and Technology, 13, 125–134. https://doi.org/10.1007/s13762-015-0851-9
Quesada, E., et al. (2004). Moderately halophilic, exopolysaccharide-producing bacteria. In A. Ventosa (Ed.), Halophilic microorganisms (pp. 297–314). Springer. https://doi.org/10.1007/978-3-662-07656-9_22
Roohi, A., Ahmed, I., Iqbal, M., & Jamil, M. (2012). Preliminary isolation and characterization of halotolerant and halophilic bacteria from salt mines of Karak, Pakistan. Pakistan Journal of Botany, 44, 365–370.
Studholme, D. J., Jackson, R. A., & Leak, D. J. (1999). Phylogenetic analysis of transformable strains of thermophilic Bacillus species. FEMS Microbiology Letters, 172, 85–90. https://doi.org/10.1111/j.1574-6968.1999.tb13454.x
Tamura, K., Nei, M., & Kumar, S. (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences, 101, 11030–11035. https://doi.org/10.1073/pnas.0404206101
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729. https://doi.org/10.1093/molbev/mst197
Tan, X., Acquah, I., Liu, H., Li, W., & Tan, S. (2019). A critical review on saline wastewater treatment by membrane bioreactor (MBR) from a microbial perspective. Chemosphere. https://doi.org/10.1016/j.chemosphere.2019.01.027
Torkamani, S., Shayegan, J., Yaghmaei, S., & Alemzadeh, I. (2008). Study of a newly isolated thermophilic bacterium capable of Kuhemond heavy crude oil and dibenzothiophene biodesulfurization following 4S pathway at 60 °C. Journal of Chemical Technology and Biotechnology, 83, 1689–1693. https://doi.org/10.1002/jctb.1987
Ventosa, A., Mellado, E., Sanchez-Porro, C., & Marquez, M. C. (2008). Halophilic and halotolerant micro-organisms from soils. In P. Dion & C. S. Nautiyal (Eds.), Microbiology of extreme soils (pp. 87–115). Springer. https://doi.org/10.1007/978-3-540-74231-9_5
Yeon, S.-H., Jeong, W.-J., & Park, J.-S. (2005). The diversity of culturable organotrophic bacteria from local solar salterns. International Journal of Microbiology, 43, 1–10.
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The authors are extremely thankful for the support of Higher Education Commission (HEC), Government of Pakistan for funding this research under Start-up Research Grant program, and National Research Program for Universities (NRPU Project No. 6232).
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Phulpoto, I.A., Jakhrani, B.A., Panhyar, A.A. et al. Temperate hyper-saline ecosystems induce spatial distribution and halo-thermotolerance potential in indigenous cultivable bacterial community. COMMUNITY ECOLOGY 23, 337–347 (2022). https://doi.org/10.1007/s42974-022-00111-9
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DOI: https://doi.org/10.1007/s42974-022-00111-9