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Biochar pyrolyzed at low temperature enhanced acidophilous plant growth by promoting rhizospheric microbes in a slightly alkaline urban soil

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Abstract

Biochar has been considered as a potential way to enhance acidophilous plant growth in alkaline soils. However, whether such enhancements are closely linked with biochar pyrolysis temperature and its improvements in rhizospheric soil fertility and microbial activities remains largely unknown. We performed a pot experiment to investigate changes in azalea (Rhododendron) morphology and physiology, as well as its rhizosphere soil chemical and biological properties in a slightly alkaline urban soil under the amendment of biochars that pyrolyzed at three temperatures (i.e., 350, 550 and 700 °C). Our results showed that the effects of biochars on plant growth and soil properties depended on pyrolysis temperature. In comparison with the non-amended control, 350 and 550 °C biochars showed significant promotions on the azalea growth in terms of photosynthetic characteristics, biomass, root morphology, and N and P uptake. Whereas, 700 °C biochar showed an inhibition effect on them. 350 °C biochar decreased soil pH and increased soil available P and K contents and the activities of α-glucosidase, N-β-glucosaminidase, phosphatase and sulfatase. In addition, 350 °C biochar significantly enhanced bacterial 16S rRNA and fungal 18S rRNA gene abundances in the rhizosphere soil of azalea and mycorrhizal infection. Correlation analysis indicated that soil pH, available nutrients and fungal abundance had positive associations with the enhanced plant growth parameters. Therefore, our findings suggest that biochar produced at low temperature could be a feasible strategy for enhancing acidophilous azalea growth and improving urban soil quality.

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Acknowledgements

This work was funded by the National Natural Science Foundation of China under grant number of 41807100 and 41977083; Jiangsu Agricultural Science and Technology Innovation Fund under grant number of CX (19) 3047; Jiangsu Provincial Forestry Development Special Fund [SuCaizihuan (2020) No. 26]: Rhododendron Germplasm Resources Center of Jiangsu Academy of Agricultural Sciences.

Funding

The research was supported by the National Natural Science Foundation of China (41807100 and 41977083), Jiangsu Agricultural Science and Technology Innovation Fund (CX (19) 3047) and Jiangsu Provincial Forestry Development Special Fund [SuCaizihuan (2020) No. 26].

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Huimin Zhou, Xiaoying Li, Chang Li, Lisi He, Xiaoqing Liu, Zheng Xiao, Xiaobo Sun, Gaoling Shi & Jiale Su

  2. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, Jiangsu, China

    Xiaoying Li

  3. State Key Laboratory of Subtropical Silviculture, Zhejiang A and F University, Lin’an, Hangzhou, 311300, China

    Junhui Chen

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  1. Huimin Zhou
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  2. Xiaoying Li
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  3. Chang Li
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  4. Lisi He
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  5. Xiaoqing Liu
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  6. Zheng Xiao
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  7. Xiaobo Sun
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  8. Gaoling Shi
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  9. Jiale Su
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  10. Junhui Chen
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Huimin Zhou. Prof. Jiale Su and Dr. Junhui Chen were the supervisors of the work and contributed equally to this work.

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Correspondence to Jiale Su or Junhui Chen.

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Zhou, H., Li, X., Li, C. et al. Biochar pyrolyzed at low temperature enhanced acidophilous plant growth by promoting rhizospheric microbes in a slightly alkaline urban soil. Biochar 3, 603–614 (2021). https://doi.org/10.1007/s42773-021-00114-1

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