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Antimicrobial and antibiofilm activities of Brazilian organic honey against oral microorganisms

  • Clinical Microbiology - Research Paper
  • Published:
Brazilian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Objective

To evaluate the antimicrobial activity of Brazilian honeys against oral microorganisms. Design: Organic honeys (OH-1 to OH-8) were diluted (%-w/v) and sterilized by filtration. Antimicrobial activity was defined by determining MIC and CBM against oral Streptococcus. The component responsible for the antimicrobial action was defined by a catalase assay. Antibiofilm activity was evaluated against the monospecies biofilm of Streptococcus mutans  (ATCC 700610).

Results

OHs showed antimicrobial activity principally OH-1, OH-2, OH-3, and OH-7 with MIC values ​​ranging between 10 and 25%. The mechanism of action occurs mainly by hydrogen peroxide produced by honey enzymes. OH-1, OH-2, and OH-7 showed total biofilm destruction at low concentrations.

Conclusion

Brazilian honeys have promising antimicrobial and antibiofilm activity with the potential to control oral microbiota.

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Acknowledgements

The authors are thankful to Breyer e Cia Ltda. — Produtos das Abelhas for providing the organic honey samples used in this study.

Funding

This work was supported by the [Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 1]; under Grant [number: 88887476194/2020-00]; [Conselho Nacional de Desenvolvimento Científico e Tecnológico 2] under Grant [141129/2017-4] and under Grant [306673/2019-3].

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Authors and Affiliations

  1. Department of Biosciences, Piracicaba Dental School, University of Campinas (UNICAMP), Av. Limeira, 901 - Areião, Piracicaba, SP, 13414-903, Brazil

    Diego Romário-Silva, Bruno Bueno-Silva & Pedro Luiz Rosalen

  2. Graduate Program in Integrated Dental Sciences, School of Dentistry, University of Cuiabá, Cuiabá, MT, 78065-900, Brazil

    Diego Romário-Silva, Fabiane Cruz-Vieira & Priscila Vieira da Silva

  3. School of Dentistry, Federal University of Alfenas (Unifal-MG), Alfenas, MG, Brazil

    Marcelo Franchin

  4. Department of Agri-Food Industry, Food and Nutrition, “Luiz de Queiroz” College of Agriculture, University of São Paulo (USP), Piracicaba, SP, 13418-900, Brazil

    Severino Matias Alencar

  5. Dental Research Division, Guarulhos University, Guarulhos, SP, 07023-070, Brazil

    Janaína de Cássia Orlandi Sardi

  6. Department of Pharmacy, Center of Biological and Health Sciences, State University of Paraíba, Campina Grande, Piracicaba, PB, 58429-500, Brazil

    Andréa Cristina Barbosa da Silva

  7. School of Pharmaceutical Sciences, Federal University of Alfenas (Unifal-MG), Alfenas, MG, Brazil

    Pedro Luiz Rosalen

Authors
  1. Diego Romário-Silva
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  2. Marcelo Franchin
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  3. Severino Matias Alencar
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  4. Bruno Bueno-Silva
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  5. Janaína de Cássia Orlandi Sardi
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  6. Andréa Cristina Barbosa da Silva
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  7. Fabiane Cruz-Vieira
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  8. Priscila Vieira da Silva
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  9. Pedro Luiz Rosalen
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Contributions

Conceptualization, Diego Romário da Silva and Pedro Luiz Rosalen; Methodology, Diego Romário da Silva, Janaína de Cássia Orlandi Sardi and Marcelo Franchin; Investigation, Diego Romário da Silva and Janaína de Cássia Orlandi Sardi; Resources, Pedro Luiz Rosalen and Severino Matias Alencar; Data Curation, Diego Romário da Silva; Writing – Original Draft Preparation, Diego Romário da Silva, Andréa Cristina Barbosa da Silva and Fabiane Cruz Vieira; Writing – Review & Editing, Diego Romário da Silva and Pedro Luiz Rosalen.; Supervision, Pedro Luiz Rosalen; Project Administration, Pedro Luiz Rosalen; Funding Acquisition, Pedro Luiz Rosalen.

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Correspondence to Pedro Luiz Rosalen.

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Romário-Silva, D., Franchin, M., Alencar, S.M. et al. Antimicrobial and antibiofilm activities of Brazilian organic honey against oral microorganisms. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01343-9

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