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The multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera: Platygastridae)

  • Soil and Agriculture - Research Paper
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Abstract

Wasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector.

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Funding

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant number 303479/2022–1) and Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF, grant number 00193.00001750/2022–66).

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  1. Laboratory of Insect Virology, Cell Biology Department, University of Brasilia, Brasilia, DF, 70910-900, Brazil

    Ethiane Rozo dos Santos, Brenda Rabelo de Camargo & Daniel M. P. Ardisson-Araújo

  2. Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasilia, DF, 70910-900, Brazil

    Leonardo Assis da Silva & Bergmann Morais Ribeiro

  3. Laboratory of Chemical Ecology, EMBRAPA Genetic Resources and Biotechnology, Brasília, DF, 70770-900, Brazil

    Raul Alberto Laumann

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42770_2024_1340_MOESM1_ESM.tif

Supplementary file1 (TIF 18360 KB) Figure S1. ML phylogenetic inference of the evolutionary relationship among Halyopmorpha halys virus (HhV) isolates to other iflaviruses, dicistroviruses, and secoviruses. Members of Secoviridae were used to root the tree. The tree was colored in relation to the taxonomic order of the host. The iflaviruses found in insectivores were painted yellow and those with unknown host are black

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dos Santos, E.R., de Camargo, B.R., da Silva, L.A. et al. The multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera: Platygastridae). Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01340-y

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  • DOI: https://doi.org/10.1007/s42770-024-01340-y

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