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Stable isotope labeling as a promising tool for rapid drug susceptibility testing in Neisseria gonorrhoeae

  • Clinical Microbiology - Research Paper
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Abstract

The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae, thereby underlining the significance of rapid and accurate prediction of drug resistance as an indispensable need of the hour. In the present study, we optimized and evaluated a stable isotope labeling-based approach using the MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry) for rapid and reliable detection of ciprofloxacin and azithromycin resistance in N. gonorrhoeae. All the isolates were cultured under three varied condition setups viz. medium supplemented with normal lysine, heavy lysine (isotope), and heavy lysine along with the antibiotics (ciprofloxacin/azithromycin), respectively. After incubation, spectra were acquired using the MALDI-TOF MS which were further screened for unique patterns (media-specific spectra) to differentiate drug-susceptible and resistant isolates. The results of the stable isotope labeling assay were comparable to the results of phenotypic methods used for susceptibility testing.

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Acknowledgements

The authors duly acknowledge the Department of Medical Microbiology, PGIMER, Chandigarh for providing all the necessary facilities.

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Authors and Affiliations

  1. Department of Medical Microbiology, PGIMER, Chandigarh, 160012, India

    Rajneesh Dadwal, Saikat Paul, Parakriti Gupta, Rakesh Yadav, A. K. Ghosh, M. R. Shivaprakash & Sunil Sethi

  2. Department of Microbiology, AIIMS, New Delhi, India

    Seema Sood

  3. Department of Obstetrics and Gynaecology, PGIMER, Chandigarh, 160012, India

    Shalini Gainder

Authors
  1. Rajneesh Dadwal
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  2. Saikat Paul
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  3. Parakriti Gupta
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  4. Rakesh Yadav
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  5. Seema Sood
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  6. A. K. Ghosh
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  7. M. R. Shivaprakash
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  8. Shalini Gainder
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  9. Sunil Sethi
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Contributions

RD: performing experiments, conceptualization, data analysis, and manuscript writing; SP: data analysis and manuscript writing/correction; PG: manuscript writing/correction; RY: manuscript correction; SSood: supervision; AG: conceptualization and supervision; MRS: conceptualization and supervision; SG: conceptualization and supervision; SS: conceptualization, supervision, and manuscript correction.

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Correspondence to Sunil Sethi.

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Dadwal, R., Paul, S., Gupta, P. et al. Stable isotope labeling as a promising tool for rapid drug susceptibility testing in Neisseria gonorrhoeae. Braz J Microbiol 54, 1819–1825 (2023). https://doi.org/10.1007/s42770-023-00996-2

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  • DOI: https://doi.org/10.1007/s42770-023-00996-2

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