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Identification of natural CTXM-15 inhibitors from aqueous extract of endophytic bacteria Cronobactersakazaki

  • Clinical Microbiology - Research Paper
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Abstract

Nyctanthes arbor-tristis is one of India’s valuable and populous medicinal plants which belongs to the family Oleaceae, and widely recognize as night jasmine. Over the years till date, different parts of the plant are used to treat or cure different ailments via various means of traditional medicine. Endophytes are organisms that live in the cell or body of other organisms with no apparent negative impact on the host which they inhabit and are of great source of novel bioactive compounds possessing important economic value. Secondary metabolites were identified in the aqueous extract of Cronobactersakazakii through quantitative phytochemical and GC–MS analysis. Antibacterial activity of the extract against clinical and ATCC strains of E. coli was assessed. Biological activity spectra of these compounds were predicted and categorized either as probably active (Pa) or probably inactive (Pi). Drug-likeness of bioactive compounds was determined as well as their ability to target protein (CTXM-15) responsible for antibiotic resistance in Gram-negative bacteria. Results revealed the presence of active compounds with pharmacological activities and considerable pharmacokinetics parameters. In addition, ligand–protein interactions of compounds with CTXM-15 proteins were identified. These results suggest that bioactive compounds of endophytic Cronobactersakazakii could contain novel chemical entities for the development of antibiotics against pathogenic microbes and other drugs for the amelioration of several infections.

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Acknowledgements

All authors are thankful to B.S. Abdur Rahman Crescent Institute of Science and Technology for providing the facilities for study.

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  1. School of Life Sciences, B. S Abdul Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, India

    M. Love Edet & S. Hemalatha

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SH conceived and designed the study. LEM performed the experiment, collected data, analyzed and interpreted data, and drafted the first manuscript. SH reviewed and edited the article. Both authors agreed to the final approval of the version to be submitted.

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Correspondence to S. Hemalatha.

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Edet, M.L., Hemalatha, S. Identification of natural CTXM-15 inhibitors from aqueous extract of endophytic bacteria Cronobactersakazaki. Braz J Microbiol 54, 827–839 (2023). https://doi.org/10.1007/s42770-023-00945-z

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