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Detection of virulence, antimicrobial resistance, and heavy metal resistance properties in Vibrio anguillarum isolated from mullet (Mugil cephalus) cultured in Korea

  • Veterinary Microbiology - Research Paper
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Abstract

In the present study, we identified and characterized 22 strains of V. anguillarum from 145 samples of mullets (Mugill cephallus) cultured in several fish farms in South Korea. They were subjected to pathogenicity tests, antimicrobial susceptibility test, and broth dilution test to detect virulence markers, antimicrobial resistance, and heavy metal resistance properties. All the isolates showed amylase and caseinase activity, followed by gelatinase (90.9%), DNase (45.5%), and hemolysis activities (α = 81.1% and β = 18.2%). The PCR assay revealed that isolates were positive for VAC, ctxAB, AtoxR, tdh, tlh, trh, Vfh, hupO, VPI, and FtoxR virulence genes at different percentages. All the isolates showed multi-drug resistance properties (MAR index ≥ 0.2), while 100% of the isolates were resistant to oxacillin, ticarcillin, streptomycin, and ciprofloxacin. Antimicrobial resistance genes, qnrS (95.5%), qnrB (86.4%), and StrAB (27.3%), were reported. In addition, 40.9% of the isolates were cadmium-tolerant, with the presence of CzcA (86.4%) heavy metal resistance gene. The results revealed potential pathogenicity associated with V. anguillarum in aquaculture and potential health risk associated with consumer health.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We appreciate Professor Shin Gee-Wook of Chonbuk National University’s Bio-Safety Research Institute and College of Veterinary Medicine in South Korea for providing the bacterial strains utilized in this investigation.

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  1. Laboratory of Aquatic Animal Medicine, Veterinary Medical Center, College of Veterinary Medicine, Chungbuk National University, Chungdae-Ro 1, Seowon-Gu, Chungbuk, 28644, Cheongju, South Korea

    P. M. Kumarage, Sana Majeed, L. A. D. S. De Silva & Gang-Joon Heo

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PMK and GJH contributed to the conception and design of the study. Material preparation, data collection, and analysis were performed by PMK, SM, LADSDS, and GJH. The first draft of the manuscript was written by PMK, and all authors contributed to reviewing and editing the manuscript. All authors read and approved the final manuscript.

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Correspondence to Gang-Joon Heo.

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Kumarage, P.M., Majeed, S., De Silva, L.A.D.S. et al. Detection of virulence, antimicrobial resistance, and heavy metal resistance properties in Vibrio anguillarum isolated from mullet (Mugil cephalus) cultured in Korea. Braz J Microbiol 54, 415–425 (2023). https://doi.org/10.1007/s42770-023-00911-9

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  • DOI: https://doi.org/10.1007/s42770-023-00911-9

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