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Antibacterial activity of biosynthesized silver nanoparticles (AgNps) against Pectobacterium carotovorum

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

In a bioprospecting study of paramo soils cultivated with potato (Solanum tuberosum), 50 fungal isolates were obtained and evaluated for their nitrate reductase (NR) activity, given the role played by this enzyme in the biosynthesis of silver nanoparticles (AgNps). Five isolates strain with high NR activity belonging to Penicillium simplicissimum, Aspergillus niger, and Fusarium oxysporum species were selected, verifying the presence of the NR enzyme in their enzymatic extract. Later, these strains showed the ability to biosynthesize AgNps with distorted spherical shapes and sizes ranging from 15 to 45 nm. Subsequently, an antibiosis test was carried out by the agar diffusion method using glass fiber disks against the phytopathogenic agent Pectobacterium carotovorum, finding halos of inhibition of bacterial growth up to 15.3 mm using a 100 ppm solution of the AgNps obtained from F. oxysporum. These results contribute to generating the basis of a new alternative for the control of this phytopathogenic agent of potato, challenging to manage by traditional methods and of relevance at the post-harvest level.

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Acknowledgements

To the Government of Boyacá for the call 733 of 2015 for high-level human capital formation for the Department of Boyacá for funding the Ph.D. fellowship and to the Ministry of Science, Technology and Innovation of Colombia.

Funding

This study was financed with resources from the Science, Technology, and Innovation Fund of the General Royalties System FCTeI-SGR attached to the Department of Boyacá and through the HERMES 47144 project of the National University of Colombia; “Biosynthesis of silver nanoparticles from rhizospheric fungi and its immobilization in a natural fiber for in vitro control of phytopathogenic bacteria (Pectobacterium carotovorum)”.

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Authors and Affiliations

  1. Doctorado en Biotecnología UN, Grupo de Investigación en Macromoléculas UN, Grupo de Investigación Biología Ambiental UPTC, Grupo de investigación Gestión Ambiental- Universidad de Boyacá, Universidad Nacional de Colombia, Bogotá, Colombia

    Mayra Eleonora Beltrán Pineda

  2. Grupo de Investigación Biología Ambiental, Universidad Pedagógica y Tecnológica de Colombia, Tunja, Colombia

    Luz Marina Lizarazo Forero

  3. Grupo de Investigación en Macromoléculas, Universidad Nacional de Colombia, Bogotá, Colombia

    Cesar Augusto Sierra Avila

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  1. Mayra Eleonora Beltrán Pineda
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  2. Luz Marina Lizarazo Forero
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  3. Cesar Augusto Sierra Avila
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Contributions

Mayra Beltrán-Pineda wrote the article and carried out the isolation, identification, and selection of promising fungi for AgNp’s biosynthesis; performed the biosynthesis of AgNps; and evaluated their antibacterial activity against the phytopathogenic agent P. carotovorum. Luz Marina Lizarazo-Forero and Cesar Augusto Sierra-Avila contributed to the design and supervision of the experimental work and the review of the article.

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Correspondence to Luz Marina Lizarazo Forero.

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Pineda, M.E.B., Lizarazo Forero, L.M. & Sierra Avila, C.A. Antibacterial activity of biosynthesized silver nanoparticles (AgNps) against Pectobacterium carotovorum. Braz J Microbiol 53, 1175–1186 (2022). https://doi.org/10.1007/s42770-022-00757-7

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