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Fractionation of hexane extracts from Achyrocline satureioides and their biological activities against Paenibacillus larvae

  • Veterinary Microbiology - Research Paper
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Abstract

Previous studies carried out in our laboratory described the antimicrobial activity of the whole hexanic extract (HE) of Achyrocline satureioides (Lam.) DC against Paenibacillus larvae, the causal agent of American Foulbrood (AFB) a disease of the honey bee larvae. In this study, the HE was partitioned into five main fractions by chromatographic techniques leading to the isolation of four known compounds: two prenylated phloroglucinol α-pyrones (1 and 3), 5,7-dihydroxy-3,8-dimethoxyflavone (gnaphaliin A) (2), and 23-methyl-6-O-demethylauricepyrone (4). Isolated compounds were further analyzed towards structural elucidation using 1H RMN and 13C RMN spectroscopic techniques. For the first time, the antimicrobial activity of the isolated compounds was evaluated against P. larvae strains by broth microdilution method and compared with that of the whole HE. Compounds 1–4 displayed minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values ranging between 0.07 and 62.5 μg/mL and 0.26 and 12.5 μg/mL, respectively. The lowest MIC and MBC values were obtained with compounds 3 and 4, respectively. The antimicrobial activity of each single compound and the combination of them showed that the presence of all compounds is needed for the antimicrobial efficacy of whole HE.

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Funding

This study was financially supported by Agencia Nacional de Promoción Científica and Secretaría de Ciencia y Técnica from the Universidad Nacional de Río Cuarto.

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Authors and Affiliations

  1. Departamento de Química, Instituto Para El Desarrollo Agroindustrial Y de La Salud (IDAS, UNRC-CONICET), Facultad de Ciencias Exactas Físico-Químicas Y Naturales, Universidad Nacional de Río Cuarto, ruta 36, km 601, X5804BYA, Río Cuarto, Córdoba, Argentina

    Natalia Tonello, Diana Pimentel Betancurt, Juan Miguel Marioli, Marcela Beatriz Moressi & Fabiana D’Eramo

  2. Departamento de Microbiología E Inmunología, Instituto de Biotecnología Ambiental Y Salud (INBIAS, UNRC-CONICET), Facultad de Ciencias Exactas Físico-Químicas Y Naturales, Universidad Nacional de Río Cuarto, ruta 36, km 601, X5804BYA, Río Cuarto, Córdoba, Argentina

    Carlos Leonel Huallpa & María de las Mercedes Oliva

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  1. Natalia Tonello
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  2. Diana Pimentel Betancurt
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  7. Fabiana D’Eramo
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Contributions

Each author has contributed significantly to this work. NT performed the experiments and analyzed the data, DP and CH performed the experiments, JM designed the experiments, MM supervised the experiments, MO supervised the experiments and edited the manuscript, and FD wrote the manuscript and edited the manuscript. All authors have reviewed, discussed, edited, and approved the final manuscript.

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Correspondence to Fabiana D’Eramo.

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Tonello, N., Pimentel Betancurt, D., Huallpa, C.L. et al. Fractionation of hexane extracts from Achyrocline satureioides and their biological activities against Paenibacillus larvae. Braz J Microbiol 53, 1645–1655 (2022). https://doi.org/10.1007/s42770-022-00736-y

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