Abstract
In the past decade, researchers have focused on the emergence of drug resistance in fungal pathogens such as Candida albicans, also considered as pathobionts that occur harmlessly in the human body but could potentially be triggered to cause diseases. The increasing rate of antifungal resistance in commensal gut fungi is alarming and should be further investigated. Here, we report seven novel MLST (Multi Locus Sequence Typing) genotypes of multi-drug resistant C. albicans isolates obtained from participants of a community study in Segamat, a district in the state of Johor, Malaysia. A total of eight C. albicans were isolated from four individuals, which were found to express high resistance against fluconazole, itraconazole, voriconazole and 5-fluorocytosine antifungals. MLST was performed to assess the clonal relatedness of these drug resistant isolates among themselves and against other strains isolated from other geographical regions. The novel MLST C. albicans sequence types suggest significant genetic changes compared to previous genotypes.
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Acknowledgements
The authors wish to thank Monash University Malaysia, the Tropical Medicine and Biology Multidisciplinary Platform (Monash University Malaysia) and the South East Asia Community Observatory (Segamat, State of Johor, Malaysia) for their support.
Funding
This study was supported by Monash University Malaysia multidisciplinary project funding [LG-2017–01-SCI], Monash University Australia funding [SCI/MUA/02–2019/001], Monash University Malaysia School of Science and the Tropical Medicine and Biology Multidisciplinary Platform.
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Supplementary Information
Supplementary file1
Minimum inhibitory concentration (MIC) values obtained with the community isolates and control strains can be found in Table S1. (DOCX 17 kb)
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Huët, M.A.L., Muzahid, N.H., Lee, C.Z. et al. Molecular typing of multi-drug resistant Candida albicans isolated from the Segamat community, Malaysia. Braz J Microbiol 52, 2351–2356 (2021). https://doi.org/10.1007/s42770-021-00558-4
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DOI: https://doi.org/10.1007/s42770-021-00558-4