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Evaluation of biosurfactant production potential of Lysinibacillus fusiformis MK559526 isolated from automobile-mechanic-workshop soil

  • Environmental Microbiology - Research Paper
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A Correction to this article was published on 15 March 2021

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Abstract

Background

Biosurfactants are amphipathic biological compounds with surface active potential and are produced by many microorganisms. Biosurfactant production by Lysinibacillus fusiformis MK559526 isolated from automobile-mechanic-shop soil was investigated with a view to assessing its potential for production and potential for optimization.

Materials and methods

Effects of carbon and nitrogen sources, pH, temperature and incubation periods on biosurfactant production were evaluated with a view to optimizing the processes. Fourier Transform Infra-Red absorption peaks and Gas chromatography mass spectrometry were used to determine the functional groups of the chemical make-up and the chemical profile of the biosurfactant respectively.

Results

Lysinibacillus fusiformis surfactant had emulsification index of 65.15 ± 0.35 %, oil displacement of 2.7 ± 0.26 mm, zone of haemolysis of 7.3 ± 0.16 mm and a positive drop collapse test. Optimized culture conditions for biosurfactant production: temperature, 35 ºC; pH, 7.0; starch solution, 40 g/L and urea, 1.5 g/L showed a reduction in surface tension to 28.46 ± 1.11 mN/m and increased emulsification index to 93.80 ± 0.41 %. Maximum biosurfactant production of 2.92 ± 0.04 g/L was obtained after 72 h. The biosurfactant contained peptides and fatty acids. The predominant fatty acid was 9-Octadecenoic acid (80.80%).

Conclusions

The above results showing high emulsification potential and remarkable reduction in the surface tension are good biosurfactant attributes. Consequently, Lysinibacillus fusiformis MK559526 is a good candidate for biosurfactant production.

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Authors and Affiliations

  1. Department of Science Laboratory Technology, Federal College of Forestry, Jos, Nigeria

    Walter Chinaka John

  2. Department of Microbiology, Federal University of Agriculture, Makurdi, Nigeria

    Innocent Okonkwo Ogbonna & Grace M. Gberikon

  3. Department of Botany, Federal University of Agriculture, Makurdi, Nigeria

    Charles Chidozie Iheukwumere

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  1. Walter Chinaka John
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  3. Grace M. Gberikon
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  4. Charles Chidozie Iheukwumere
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Correspondence to Innocent Okonkwo Ogbonna.

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The original online version of this article was revised: in the original publication of the article, the first author’s name was incorrect. The correct name is Walter Chinaka John , not Walter Chika John.

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John, W.C., Ogbonna, I.O., Gberikon, G.M. et al. Evaluation of biosurfactant production potential of Lysinibacillus fusiformis MK559526 isolated from automobile-mechanic-workshop soil. Braz J Microbiol 52, 663–674 (2021). https://doi.org/10.1007/s42770-021-00432-3

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