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Enterobacter sp. strain Fs-11 adapted to diverse ecological conditions and promoted sunflower achene yield, nutrient uptake, and oil contents

  • Environmental Microbiology - Research Paper
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Abstract

Plant growth–promoting rhizobacteria are under extensive investigation to supplement the chemical fertilizers due to cost-effective and eco-friendly nature. However, their consistency in heterogeneous soil and diverse ecological settings is unclear. The current study presents in vitro and field evaluation of pre-characterized PGPR strain Enterobacter sp. Fs-11 (GenBank accession # GQ179978) in terms of its potential to enhance sunflower yield and oil contents under diverse environmental conditions. Under in vitro conditions, strain Fs-11 showed optimal growth at a range of temperature (15 to 40 °C) and pH values (6.5 to 8.5). Extracellular and intracellular localizations of the strain Fs-11 in sunflower root cortical cells through transmission electron microscopy confirmed its epiphytic and endophytic colonization patterns, respectively. In field experiments, conducted at three different agro-climatic locations, inoculation of strain Fs-11 at 50% reduced NP fertilizer resulted in a significant increase in growth, achene yield, nutrient uptake, and oil contents. Inoculation also responded significantly in terms of increase in mono- and polyunsaturated fatty acids (oleic and linoleic acids, respectively) without rising saturated fatty acid (palmitic and stearic acids) contents. We concluded that Enterobacter sp. Fs-11 is a potential candidate for biofertilizer formulations to supplement chemical fertilizer requirements of sunflower crop under diverse climatic conditions.

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Acknowledgements

We are also thankful to Dr. Iftikhar Ali (Deputy Chief Scientist, NIFA, Peshawar) for providing space to conduct field trial and for conducting achene oil and fatty acid analysis. We also appreciate the efforts of Dr. Nasir Rahim (Assistant professor, UPR) for providing resources to conduct field experiment at UPR.

Funding

The authors are grateful to Higher Education Commission (HEC), Pakistan, for grant of funds for this project under HEC indigenous 5000 PhD Fellowship Program.

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Authors and Affiliations

  1. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, 38000, Pakistan

    Muhammad Shahid & Mohsin Tariq

  2. Microbial Physiology Laboratory, National Institute for Biotechnology & Genetic Engineering (NIBGE), Faisalabad, Pakistan

    Muhammad Shahid, Sohail Hameed, Mohsin Tariq, Khadim Hussain & Amanat Ali

  3. Department of Soil and Environmental Sciences, The University of Poonch, Rawalakot, Azad Jammu & Kashmir, Pakistan

    Mohsin Zafar

  4. Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus, Islamabad, Pakistan

    Muhammad Tahir

  5. College of Agriculture, Bahauddin Zakariya University, Bahadur Sub-Campus, Layyah, Pakistan

    Muhammad Ijaz

  6. Soil Science Division, Nuclear Institute for Agriculture (NIA), Tandojam, Pakistan

    Amanat Ali

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  1. Muhammad Shahid
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Shahid, M., Hameed, S., Zafar, M. et al. Enterobacter sp. strain Fs-11 adapted to diverse ecological conditions and promoted sunflower achene yield, nutrient uptake, and oil contents. Braz J Microbiol 50, 459–469 (2019). https://doi.org/10.1007/s42770-019-00061-x

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