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Pathological and virological features of skin lesions caused by BVDV in cattle

  • Veterinary Microbiology - Research Paper
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Abstract

Dermatitis might occur in mucosal disease (MD) caused by bovine viral diarrhea virus (BVDV). This study describes the pathological and virological features of skin lesions associated with BVDV infection in four persistently infected (PI) cattle. Skin samples were reprocessed for histopathology and IHC. BVDV isolates were obtained and were genetically characterized. In addition to upper alimentary system ulcerative lesions, all cattle (one outbreak and three individual cases) presented focal crusty and ulcerative lesions affecting the mucocutaneous and skin-horn junctions, interdigital clefts, pastern, and areas surrounding the dewclaws and diffuse thickened skin within 7–20 days of infection. Microscopic analysis revealed parakeratotic hyperkeratosis and single-cell keratinocyte death, accompanied by ballooning degeneration and spongiosis in the epidermis, as well as intraepithelial and subcorneal pustules. IHC showed BVDV antigen in the cytoplasm of keratinocytes undergoing individual cell death. Phylogenetic analysis revealed that the isolates from cattle #1, #2, and #4 belonged to BVDV-1a, whereas that from cattle #3 belonged to BVDV-1d. Cytopathic BVDV was isolated from cattle #2 and #3 (MD), and non-cytopathic BVDV was isolated from cattle #1 and #4. Thus, BVDV infection might cause acute disease, characterized by skin and upper alimentary system ulcerative lesions, in both MD and PI cattle.

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Acknowledgements

The authors are thankful to the technician of Cíntia de Lorenzo for performing the IHC of this study.

Funding sources

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnólogico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Faculdade de Veterinária, Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil

    Matheus Viezzer Bianchi, Suyene Oltramari de Souza, Guilherme Konradt, David Driemeier & Saulo Petinatti Pavarini

  2. Faculdade de Veterinária, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil

    Simone Silveira, Ana Cristina Sbaraini Mósena & Cláudio Wageck Canal

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  1. Matheus Viezzer Bianchi
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  2. Simone Silveira
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  3. Ana Cristina Sbaraini Mósena
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  4. Suyene Oltramari de Souza
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  5. Guilherme Konradt
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  6. Cláudio Wageck Canal
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  7. David Driemeier
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  8. Saulo Petinatti Pavarini
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Correspondence to Matheus Viezzer Bianchi.

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Bianchi, M.V., Silveira, S., Mósena, A.C.S. et al. Pathological and virological features of skin lesions caused by BVDV in cattle. Braz J Microbiol 50, 271–277 (2019). https://doi.org/10.1007/s42770-018-0019-0

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  • DOI: https://doi.org/10.1007/s42770-018-0019-0

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