Abstract
With the rise of optogenetic manipulation of neurons, the effects of optogenetic heating on temperature-sensitive physiological processes, and the damage to surrounding tissues have been neglected. This manuscript reports the fabrication of a highly temperature-sensitive semi-interpenetrating optical hydrogel fiber (TSOHF) using the integrated dynamic wet-spinning technique. TSOHF exhibits a structural tunable diameter, clear core/sheath structure, tunable temperature-sensitivity, excellent light propagation property (0.35 dB cm− 1, 650 nm laser light), and good biocompatibility (including tissue-like Young’s modulus, stable dimensional stability, and low cytotoxicity). Based on these properties, a potential application of optogenetic regulation of neural tissue (hypoglossal nerve), with controllable temperature using TSOHF was designed and performed. Further, this work provides new insight into molecular design and a practical approach to continually manufacture a temperature-sensitive hydrogel optical fiber for applications in intelligent photomedicine.
Graphical Abstract
Similar content being viewed by others
References
Jiang S, Wu X, Rommelfanger JN, Ou ZH, Hong GS. Shedding light on neurons: optical approaches for neuromodulation. Natl Sci Rev. 2022;9:nwac007.
Henry R, Deckert M, Guruviah V, Schmidt B. Review of neuromodulation techniques and technological limitations. Iete Tech Rev. 2016;33:368–77.
Park S, Yuk H, Zhao RK, Yim YS, Woldeghebriel EW, Kang J, Canales A, Fink Y, Choi BG, Zhao XH, Anikeeva P. Adaptive and multifunctional hydrogel hybrid probes for long-term sensing and modulation of neural activity. Nat Commun. 2021;12:3435.
Vitale F, Summerson RS, Aazhang B, Kemere C, Pasquali M. Neural stimulation and recording with bidirectional, soft carbon nanotube fiber microelectrodes. ACS Nano. 2015;9:4465–74.
Nazempour R, Zhang BZ, Ye ZY, Yin L, Lv XL, Sheng X. Emerging applications of optical fiber-based devices for brain research. Adv Fiber Mater. 2021;4:24–42.
Boyden SE, Zhang F, Bamberg E, Nagel G, Deisseroth K. Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci. 2005;8:1263–8.
Deisseroth K. Optogenetics. Nat Methods. 2011;8:26–9.
Yang Q, Song D, Xie Z, He G, Zhao J, Wang Z, Dong Z, Zhang H, Yang L, Jiang M, Wu Y, Shi Q, Li J, Yang J, Bai Z, Quan Z, Qing H. Optogenetic stimulation of CA3 pyramidal neurons restores synaptic deficits to improve spatial short-term memory in APP/PS1 mice. Prog Neurobiol. 2022;209:102209.
Mickle AD, Won SM, Noh KN, Yoon J, Meacham KW, Xue Y, McIlvried LA, Copits BA, Samineni VK, Crawford KE, Kim DH, Srivastava P, Kim BH, Min S, Shiuan Y, Yun Y, Payne MA, Zhang J, Jang H, Li Y, Lai HH, Huang Y, Park SI, Gereau RW, Rogers JA. A wireless closed-loop system for optogenetic peripheral neuromodulation. Nature. 2019;565(7739):361–5.
Ward PJ, Jones LN, Mulligan A, Goolsby W, Wilhelm JC, English AW. Optically-induced neuronal activity is sufficient to promote functional motor axon regeneration in vivo. PLoS ONE. 2016;11(5):16.
Owen SF, Liu MH, Kreitzer AC. Thermal constraints on in vivo optogenetic manipulations. Nat Neurosci. 2019;22:1061–5.
Stujenske JM, Spellman T, Gordon JA. Modeling the spatiotemporal dynamics of light and heat propagation for in vivo optogenetics. Cell Rep. 2015;12:525–34.
Yizhar O, Fenno EL, Davidson JT, Mogri M, Deisseroth K. Optogenetics in neural systems. Neuron. 2011;71:9–34.
Yang F, Zheng J. High temperature sensitivity is intrinsic to voltage-gated potassium channels. Elife. 2014;3:e03255.
Sabatini BL, Regehr WG. Timing of neurotransmission at fast synapses in the mammalian brain. Nature. 1996;384:170–2.
Moser E, Mathiesen I, Andersen P. Association between brain temperature and dentate field potentials in exploring and swimming rats. Science. 1993;259:1324–6.
Peters K. Polymer optical fiber sensors—a review. Smart Mater Struct. 2011;20:013002.
Canales A, Jia XT, Froriep PU, Koppes AR, Tringides MC, Selvidge J, Lu C, Hou C, Wei L, Fink Y, Anikeeva P. Multifunctional fibers for simultaneous optical, electrical and chemical interrogation of neural circuits in vivo. Nat Biotechnol. 2015;33:277–84.
Lou Y, Wu D, Pang Y. Optical trapping and manipulation using optical fibers. Adv Fiber Mater. 2019;4:83–100.
Choi M, Choi JW, Kim S, Nizamoglu S, Hahn SK, Yun SH. Light-guiding hydrogels for cell-based sensing and optogenetic synthesis in vivo. Nat Photonics. 2013;7:987–94.
Nizamoglu S, Gather CM, Humar M, Choi M, Kim S, Kim KS, Hahn SK, Scarcelli G, Randolph M, Redmond WR, Yun SH. Bioabsorbable polymer optical waveguides for deep-tissue photomedicine. Nat Commun. 2016;7:10374.
Yuk H, Lu B, Zhao XH. Hydrogel bioelectronics. Chem Soc Rev. 2019;48:1642–67.
Yetisen AK, Jiang N, Fallahi A, Montelongo Y, Ruiz-Esparza UG, Tamayol A, Zhang YS, Mahmood I, Yang SA, Kim KS, Butt H. Glucose-sensitive hydrogel optical fibers functionalized with phenylboronic acid. Adv Mater. 2017;29:1606380.
Chen T, Qiao XL, Wei PL, Chen GY, Mugaanire TI, Hou K, Zhu MF. Tough gel-fibers as strain sensors based on strain–optics conversion induced by anisotropic structural evolution. Chem Mater. 2020;32:9675–87.
Choi M, Humar M, Kim S, Yun SH. Step-index optical fiber made of biocompatible hydrogels. Adv Mater. 2015;27:4081–6.
Xia MG, Wu WJ, Liu FW, Theato P, Zhu MF. Swelling behavior of thermosensitive nanocomposite hydrogels composed of oligo(ethylene glycol) methacrylates and clay. Eur Polym J. 2015;69:472–82.
Xia MG, Cheng YH, Meng ZQ, Jiang XZ, Chen ZG, Theato P, Zhu MF. A novel nanocomposite hydrogel with precisely tunable UCST and LCST. Macromol Rapid Commun. 2015;36:477–82.
Sun ST, Wu PY. On the thermally reversible dynamic hydration behavior of oligo(ethylene glycol) methacrylate-based polymers in water. Macromolecules. 2012;46:236–46.
Chen GY, Wang G, Tan XR, Hou K, Meng QS, Zhao P, Wang S, Zhang JY, Zhou Z, Chen T, Cheng YH, Hsiao SB, Reichmanis E, Zhu MF. Integrated dynamic wet spinning of core-sheath hydrogel fibers for optical-to-brain/tissue communications. Natl Sci Rev. 2020;8:nwaa209.
Jiang N, Ahmed R, Rifat AA, Guo JJ, Yin YX, Montelongo Y, Butt H, Yetisen KA. Functionalized flexible soft polymer optical fibers for laser photomedicine. Adv Opt Mater. 2018;6:1701118.
Guo JJ, Liu XY, Jiang N, Yetisen KA, Yuk H, Yang CX, Khademhosseini A, Zhao XH, Yun SH. Highly stretchable, strain sensing hydrogel optical fibers. Adv Mater. 2016;28:10244–9.
Kajbafzadeh AM, Javan-Farazmand Ni, Monajemzadeh M, Baghayee A. Determining the optimal decellularization and sterilization protocol for preparing a tissue scaffold of a human-sized liver tissue. Tissue Eng Part C Methods. 2013;19:642–51.
Rashid B, Destrade M, Gilchrist MD. Mechanical characterization of brain tissue in tension at dynamic strain rates. J Mech Behav Biomed Mater. 2014;33:43–54.
Engler JA, Sen S, Sweeney HL, Discher ED. Matrix elasticity directs stem cell lineage specification. Cell. 2006;126:677–89.
Acknowledgements
This work is supported by the Shanghai Stomatological Hospital Science and Technology Talents Project (SSH-2022-KJCX-B01); National Key Research and Development Program of China (2021YFA1201302/2021YFA1201300); the National Natural Science Foundation of China (NO. 52173029; NO. 51733002; NO. 51803022); Guoyin Chen thanks for the support from the fellowship of China National Postdoctoral Program for Innovative Talents under Grant BX20220063, and Graduate Student Innovation Fund of Donghua University (CUSF-DH-D-2020038).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of Interest
The authors declare`that they have no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Chen, G., Xu, S., Zhou, Q. et al. Temperature-Gated Light-Guiding Hydrogel Fiber for Thermoregulation During Optogenetic Neuromodulation. Adv. Fiber Mater. 5, 968–978 (2023). https://doi.org/10.1007/s42765-023-00257-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42765-023-00257-9