Abstract
Plants can generate and release allelochemicals into soils under environmental stresses to compete for resources and to combat pathogens and parasites. The frequent rotation of cultivated land and the high-density cropping may result in the accumulation of allelochemicals, resulting in obstacles to continuous cropping. Biochar has been proven to improve soil quality, which can potentially reduce the formation of allelochemicals by plants, alleviating allelopathy. In this study, the formation of an allelochemical substance benzoic acid (BA), the growth indicators, and root lipid peroxidation of soybean were investigated under the influence of biochar application in soils through pot experiments. A reduced BA content was observed in the soils after biochar application, and sorption and degradation of BA by biochar could not explain this change. BA contents inside and outside the soybean root also decreased after the addition of biochar, indicating a significant reduction in BA formation by the plant. The reduced lipid peroxidation (malondialdehyde (MDA) concentration) in soybean roots and promoted plant growth indicated that biochar application greatly reduced plant BA stress. Therefore, biochar addition into the soil can reduce the generation and release of BA from soybean root, which has a positive effect on alleviating allelopathy. This study provides a new angle for a comprehensive evaluation of the environmental impact of biochar and technical support for overcoming the obstacles in continuous cropping.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
References
Chu G, Han Z, Wang Z, Kong D, Qin W, Si Y et al (2022) The sorption of sulfamethoxazole by aliphatic and aromatic carbons from lignocellulose pyrolysis. Agron 12:476. https://doi.org/10.3390/agronomy12020476
Chu G, Zhao J, Huang Y, Zhou D, Liu Y, Wu M et al (2018) Phosphoric acid pretreatment enhances the specific surface areas of biochars by generation of micropores. Environ Pollut 240:1–9. https://doi.org/10.1016/j.envpol.2018.04.003
Duke SO (2010) Allelopathy: current status of research and future of the discipline: a commentary. Allelopathy J 25:17–29. https://doi.org/10.2134/agronj2009.0183
Feng ZJ, Fan ZL, Song HP, Li KL, Lu HN, Liu Y et al (2021) Biochar induced changes of soil dissolved organic matter: the release and adsorption of dissolved organic matter by biochar and soil. Sci Total Environ 783. https://doi.org/10.1016/j.scitotenv.2021.147091
Gao Y, Liu W, Wang X, Yang L, Han S, Chen S et al (2018) Comparative phytotoxicity of usnic acid, salicylic acid, cinnamic acid and benzoic acid on photosynthetic apparatus of Chlamydomonas reinhardtii. Plant Physiol Biochem 128:1–12. https://doi.org/10.1016/j.plaphy.2018.04.037
Godlewska P, Ok YS, Oleszczuk P (2021) The dark side of black gold: ecotoxicological aspects of biochar and biochar-amended soils. J Hazard Mater 403:123833. https://doi.org/10.1016/j.jhazmat.2020.123833
Gul S, Whalen JK, Thomas BW, Sachdeva V, Deng H (2015) Physico-chemical properties and microbial responses in biochar-amended soils: mechanisms and future directions. Agric Ecosyst. Environ 206:46–59. https://doi.org/10.1016/j.agee.2015.03.015
He H, Zhu W, Noor I, Liu J, Li G (2019) Pseudomonas putida WH-B3 degrades benzoic acid and alleviates its autotoxicity to peach (Prunus persica L. batsch) seedlings grown in replanted soil. Sci Hortic 255:183–192. https://doi.org/10.1016/j.scienta.2019.05.020
Hussain M, Farooq M, Nawaz A, Al-Sadi AM, Solaiman ZM, Alghamdi SS et al (2016) Biochar for crop production: potential benefits and risks. J Soils Sediments 17:685–716. https://doi.org/10.1007/s11368-016-1360-2
Jaiswal AK, Elad Y, Graber ER, Frenkel O (2014) Rhizoctonia solani suppression and plant growth promotion in cucumber as affected by biochar pyrolysis temperature, feedstock and concentration. Soil Biol Biochem 69:110–118. https://doi.org/10.1016/j.soilbio.2013.10.051
Kirwa HK, Murungi LK, Beck JJ, Torto B (2018) Elicitation of differential responses in the root-knot nematode meloidogyne incognita to tomato root exudate cytokinin, flavonoids, and alkaloids. J Agric Food Chem 66:11291–11300. https://doi.org/10.1021/acs.jafc.8b05101
Li J, Li Q, Steinberg CEW, Zhao Q, Pan B, Pignatello JJ et al (2020) Reaction of substituted phenols with lignin char: dual oxidative and reductive pathways depending on substituents and conditions. Environ Sci Technol 54:15811–15820. https://doi.org/10.1021/acs.est.0c04991
Liao S, Pan B, Li H, Zhang D, Xing B (2014) Detecting free radicals in biochars and determining their ability to inhibit the germination and growth of corn, wheat and rice seedlings. Environ Sci Technol 48:8581–8587. https://doi.org/10.1021/es404250a
Lieke T, Zhang X, Steinberg CEW, Pan B (2018) Overlooked risks of biochars: persistent free radicals trigger neurotoxicity in Caenorhabditis elegans. Environ Sci Technol 52:7981–7987. https://doi.org/10.1021/acs.est.8b01338
Liu X, Huang B (2000) Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Sci 40:503–510. https://doi.org/10.2135/cropsci2000.402503x
Mansoor S, Kour N, Manhas S, Zahid S, Wani OA, Sharma V et al (2021) Biochar as a tool for effective management of drought and heavy metal toxicity. Chemosphere 271:129458. https://doi.org/10.1016/j.chemosphere.2020.129458
Muzell Trezzi M, Vidal RA, Balbinot Junior AA, von Hertwig Bittencourt H, da Silva Souza Filho AP, (2016) Allelopathy: driving mechanisms governing its activity in agriculture. J Plant Interact 11:53–60. https://doi.org/10.1080/17429145.2016.1159342
Nehela Y, Taha NA, Elzaawely AA, Xuan TD, Amin MA, Ahmed ME, et al (2021) Benzoic acid and its hydroxylated derivatives suppress early blight of tomato (Alternaria solani) via the induction of salicylic acid biosynthesis and enzymatic and nonenzymatic antioxidant defense machinery. J Fungi 7. https://doi.org/10.3390/jof7080663
Parra Amin JE, Cuca LE, González-Coloma A (2021) Antifungal and phytotoxic activity of benzoic acid derivatives from inflorescences of Piper cumanense. Nat Prod Res 35:2763–2771. https://doi.org/10.1080/14786419.2019.1662010
Peter S, Cornie VH, Erika M, Ronald V (2014) World reference base (WRB) for soil resources 2014. IUSS Working Group WRB. http://www.fao.org/3/i3794en/I3794en.pdf
Qin Y, Li G, Gao Y, Zhang L, Ok YS, An T (2018) Persistent free radicals in carbon-based materials on transformation of refractory organic contaminants (ROCs) in water: a critical review. Water Res 137:130–143. https://doi.org/10.1016/j.watres.2018.03.012
Qualley AV, Widhalm JR, Adebesin F, Kish CM, Dudareva N (2012) Completion of the core b-oxidative pathway of benzoic acid biosynthesis in plants. Proc Natl Acad Sci 109:16383–16388. https://sci-hub.mksa.top/10.1073/pnas.1211001109
Schulze E-D, Beck E, Buchmann N, Clemens S, Müller-Hohenstein K, Scherer-Lorenzen M (2019) Plant Ecol. Springer, Berlin. https://doi.org/10.1093/aob/mcj018
Siedt M, Schäffer A, Smith KEC, Nabel M, Roß-Nickoll M, Van Dongen JT (2021) Comparing straw, compost, and biochar regarding their suitability as agricultural soil amendments to affect soil structure, nutrient leaching, microbial communities, and the fate of pesticides. Sci Total Environ 751:141607. https://doi.org/10.1016/j.scitotenv.2020.141607
Tong YR, McNamara PJ, Mayer BK (2019) Adsorption of organic micropollutants onto biochar: a review of relevant kinetics, mechanisms and equilibrium. Environ Sci-Water Res Technol 5:821–838. https://doi.org/10.1039/C8EW00938D
Trelease SF (1942) Identification of selenium indicator species of Astragalus by germination tests. Sci 95:656–657. https://doi.org/10.1126/science.95.2478.656
Wang Z, Fang H, Wang S (2016) Benzoic acid interactions affect aquatic properties and toxicity of copper oxide nanoparticles. Bull Environ Contam Toxicol 97:159–165. https://doi.org/10.1016/j.pbi.2004.05.007
Weir TL, Park SW, Vivanco JM (2004) Biochemical and physiological mechanisms mediated by allelochemicals. Curr Opin Plant Biol 7:472–479. https://doi.org/10.1007/s00128-016-1804-9
Widhalm JR, Dudareva N (2015) A familiar ring to it: biosynthesis of plant benzoic acids. Mol Plant 8:83–97. https://doi.org/10.1016/j.molp.2014.12.001
Yang D, Liu Y, Liu S, Li Z, Tan X, Huang X et al (2016) Biochar to improve soil fertility. A review. Agron Sustainable Dev 36:36. https://link.springer.com/article/10.1007/s13593-016-0372-z
Yang H, Li X, Gao J, Tong P, Yang A, Chen H (2017) Germination-assisted enzymatic hydrolysis can improve the quality of soybean protein. J Food Sci 82:1814–1819. https://doi.org/10.1111/1750-3841.13782
Yuan Y, Bolan N, Prevoteau A, Vithanage M, Biswas JK, Ok YS et al (2017) Applications of biochar in redox-mediated reactions. Bioresour Technol 246:271–281. https://doi.org/10.1016/j.biortech.2017.06.154
Yue L, Lian F, Han Y, Bao Q, Wang Z, Xing B (2019) The effect of biochar nanoparticles on rice plant growth and the uptake of heavy metals: implications for agronomic benefits and potential risk. Sci Total Environ 656:9–18. https://doi.org/10.1016/j.scitotenv.2018.11.364
Zhang P, Sun HW, Min LJ, Ren C (2018) Biochars change the sorption and degradation of thiacloprid in soil: Insights into chemical and biological mechanisms. Environ Pollu 236:158–167. https://doi.org/10.1016/j.envpol.2018.01.030
Funding
This research was supported by the National Natural Science Foundation of China (42067055, 41977334), the Applied Basic Research Key Project of Yunnan (202001AS070015), and the Major Science and Technology Projects in Yunnan Province (202102AG050032).
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Pinyao Lan: investigation, writing-original draft; Quan Chen: investigation, funding acquisition; Meng Lu: investigation; Christian E.W. Steinberg: investigation, writing; Min Wu: investigation, writing and editing, funding acquisition; Bo Pan: writing and editing, funding acquisition.
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Highlights
• Biochar application decreases the overall BA content in soybean-soil system.
• BA sorption and degradation by biochar could not explain its concentration change.
• BA reduction relates to the reduced lipid peroxidation and improved plant growth.
• Lower-temperature biochar inhibited BA accumulation more significantly.
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Lan, P., Chen, Q., Lu, M. et al. Biochar Reduces Generation and Release of Benzoic Acid from Soybean Root. J Soil Sci Plant Nutr 23, 5026–5035 (2023). https://doi.org/10.1007/s42729-023-01534-7
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DOI: https://doi.org/10.1007/s42729-023-01534-7