Abstract
Sogatella furcifera is an important rice pest with wing dimorphism. We previously demonstrated that differentially methylated DNA fragments obtained in macropterous and brachypterous adults of S. furcifera were homologous to the 28 S ribosomal RNA (rRNA) gene, we hypothesized that the methylation levels of 28 S rRNA gene between two wing phenotypes may be different. To test this, we cloned 28 S rRNA gene, which contained 3579 bp and 6 CpG islands. Then methylation-specific primers were designed in the four CpG islands. We found that the four CpG islands were all methylated, and the methylation level remained above 40%, suggesting that the ribosomal gene was the high frequency region for the methylation in the rice pest. We also found that the methylation levels and patterns of 28 S rRNA gene were not consistent between two wing phenotypes individuals of S. furcifera. The findings imply that DNA methylation of 28 S rRNA gene may be related to wing phenotype variations in rice planthoppers, and will facilitate the epigenetic mechanism researches of insect wing dimorphism.
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References
Carvalho A, Polanco C, Lima-Brito J, Guedes-Pinto H (2010) Differential rRNA genes expression in hexaploid wheat related to NOR methylation. Plant Mol Biol Rep 28:403–412
D’Aquila P, Bellizzi D, Passarino G (2018) rRNA-gene methylation and biological aging. Aging-US 10:7–8
Edwards JR, Yarychkivska O, Boulard M, Bestor TH (2017) DNA methylation and DNA methyltransfersaes. Epigenet Chromatin 10:23. https://doi.org/10.1186/s13072-017-0130-8
Glastad KM, Hunt BG, Yi SV, Goodisman MAD (2011) DNA methylation in insects: on the brink of the epigenomic era. Insect Mol Biol 20:553–565
Glastad KM, Hunt BG, Goodisman MAD (2019) Epigenetics in insects: genome regulation and the generation of phenotypic diversity. Annu Rev Entomol 64:185–203. https://doi.org/10.1146/annurev-ento-011118-111914
Hayes AM, Lavine MD, Gotoh H, Lin XD, Lavine LC (2019) Mechanisms regulating phenotypic plasticity in wing polyphenic insects. Adv Insect Physiol 56:43–72. https://doi.org/10.1016/bs.aiip.2019.01.005
Head JA (2014) Patterns of DNA methylation in animals: an ecotoxicogival perspective. Integr Comp Biol 1:77–86
Hu CX, Fu XW, Wu KM (2017) Seasonal migration of white-backed planthopper Sogatella furcifera Horvath (Hemiptera: Delphacidae) over the Bohai Sea in northern China. J Asia-Pac Entomol 20:1358–1363. https://doi.org/10.1016/j.aspen.2017.10.004
Iwanaga K, Tojo S (1986) Effects of juvenile hormome and rearing density on wing dimorphism and oocyte development in the brown planthopper, Nilaparvata lugens. Insect Physiol 32:585–590. https://doi.org/10.1016/0022-1910(86)90076-4
Kamioka T, Iwasa Y (2017) Evolution of density-dependent wing polymorphism in insects. Evol Ecol Res 18:335–348
Kucharski R, Maleszka J, Foret S, Maleszka R (2008) Nutritional control of reproductive status in honeybees via DNA methylation. Science 319:1827–1830. https://doi.org/10.1126/science.1153069
Lee EJ, Luo JF, Wilson JM, Shi H (2013) Analyzing the cancer methylome through targeted bisulfite sequencing. Cancer Lett 340:171–178
Li KY, Hu DB, Liu FZ, Long M, Liu SY, Zhao J et al (2015) Wing patterning genes of Nilaparvata lugens identification by transcriptome analysis, and their differential expression profile in wing pads between brachypterous and macropterous morphs. J Integr Agr 14:1796–1807. https://doi.org/10.1016/S2095-3119(14)60948-5
Liang SK, Liang ZQ, Zhou XS, Chen JL, Li GH, Wang FH (2015) CpG methylated ribosomal RNA genes in relation to wing polymorphism in the rice pest Sogatella furcifera. J Asia-Pac Entomol 18:471–475
Liang ZQ, Song SY, Liang SK, Wang FH (2016) Analysis of differential proteins in two wing-type females of Sogatella furcifera (Hemiptera:Delphacidae). J Insect Sci 16:1–7
Liang AW, Zhang H, Lin J, Wang FH (2018) De novo assembly and analysis of the white-backed planthopper (Sogatella furcifera) transcriptome. J Insect Sci 11:1–7
Lin XD, Lavine LC (2018) Endocrine regulation of a dispersal polymorphism in winged insects: a short review. Curr Opin Insect Sci 25:20–24. https://doi.org/10.1016/j.cois.2017011.004
Lin XD, Yao Y, Wang B, Lavine MD, Lavine LC (2016) JNK signaling mediates wing form polymorphism in brown planthoppers (Nilaparvata lugens). Insect Biochem Molec 73:55–61. https://doi.org/10.1016/j.ibmb.2016.04.005
Lin XD, Xu YL, Jiang JR, Lavine LC, Lavine MD (2018) Host quality induces phenotypic plasticity in a wing polyphenic insect. Proc Natl Acad Sci USA 115:7563–7568. https://doi.org/10.1073/pnas.1721473115
Liu FZ, Li KY, Li J, Hu DB, Zhao J, He YP et al (2015) Apterous a modulates wing size, bristle formation and patterning in Nilaparvata lugens. Sci Rep 5:10526. https://doi.org/10.1038/srep10526
Nijhout HF, McKenna KZ (2018) The distinct roles of insulin signaling in polyphenic development. Curr Opin Insect Sci 25:58–64. https://doi.org/10.1016/j.cois.2017.11.011
Patalano S, Hore TA, Reik W (2016) Shifting behaviour: epigenetic reprogramming in eusocial insects. Curr Opin Cell Biol 24:367–373
Syobu S, Mikuriya H, Yamaguchi J, Matsuzaki M (2002) Fluctuations and factors affecting the wing-form ratio of the brown planthopper, Nilaparvata lugens stal in rice fields. Jpn J Appl Entomol Zool 46:135–143. https://doi.org/10.1303/jjaez.2002.135
Wang CY, Fei MH, Meng L, Jeffrey AH, Li BP (2020) Effects of elevated CO2 and temperature on survival and wing dimorphism of two species of rice planthoppers (Hemiptera: Delphacidae) under interaction. Pest Manag Sci 76:2087–2094. https://doi.org/10.1002/p5.5747
Xu HJ, Zhang CX (2017) Insulin receptors and wing dimorphism in rice planthoppers. Philos T R Soc B 372:20150489. https://doi.org/10.1098/rstb.2015.0489
Xu HJ, Yue J, Lu B, Zhang XC, Zhuo JC, He SF, Ma XF, Jiang YQ, Fan HW, Xu JY, Ye YX, Pan PL, Li Q, Bao YY, Nijhout HF, Zhang CX (2015) Two insulin receptors determine alternative wing morphs in planthoppers. Nature 519:464–467. https://doi.org/10.1038/nature14286
Xu JJ, Wan GJ, Hu DB, He J, Chen FJ, Wang XH et al (2016) Molecular characterization, tissue and developmental expression profiles of cryptochrome genes in wing dimorphic brown planthoppers, Nilaparvata lugens. Insect Sci 23:805–818. https://doi.org/10.1111/1744-7917.12256
Yu JL, An ZF, Liu XD (2014) Wingless gene cloning and its role in manipulating the wing dimorphism in the white-backed planthopper, Sogatella furcifera. BMC Mol Biol 15:20
Zera AJ (2003) The endocrine regulation of wing polymorphism in insects: state of the art, recent surprises, and directions. Integr Comp Biol 43:607–616. https://doi.org/10.1093/icb/43.5.607
Zera AJ (2015) Juvenile hormone and the endocrine regulation of wing polymorphism in insects: new insights from circadian and functional-genomic studies in Gryllus crickets. Physiol Entomol 41:313–326. https://doi.org/10.1111/phen.12166
Zhang HM, Liang ZB, Zhu JK (2018) Dynamics and function of DNA methylation in plants. Nat Rev Mol Cell Bio 8:489–506
Zhang CX, Brisson JA, Xu HJ (2019) Molecular mechanisms of wing polymorphism in insects. Annu Rev Entomol 64:297–314. https://doi.org/10.1146/annurev-ento-011118-112448
Zhou XS, Chen JL, Zhang M, Liang SK, Wang FH (2013) Differential DNA methylation between two wing phenotypes adults of Sogatella furcifera. Genesis 51:819–826. https://doi.org/10.1002/dvg.22722
Zhou JC, Lei C, Shi JK (2017) Tra-2 mediates cross-talk between sex determination and wing polyphenism in female Nilaparvata lugens. Genetics 207:1067–1078. https://doi.org/10.1534/genetics.117.300328
Acknowledgements
Our research was granted by the science and technology planning project of Guangzhou (grant number 202002030019) and the Natural Science Foundation of Guangdong Province (grant number 2021A1515012402).
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Feng, B., Liang, Z. & Wang, F. Cloning and differential methylation of 28 S rRNA gene from two wing phenotypes of Sogatella furcifera, an important pest in rice. Int J Trop Insect Sci 43, 1505–1512 (2023). https://doi.org/10.1007/s42690-023-01058-8
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DOI: https://doi.org/10.1007/s42690-023-01058-8