Abstract
Vector control plays a critical role in achieving reduction in the spread of dengue fever and dengue haemorrhagic fever among humans. As there are no proper medications for the treatment of dengue and the incidence of the disease is increasing every year, there is a need to develop a potential biocontrol agent for the control of dengue vector Aedes spp. Initial investigations on the bioassay studies using Petroleum Ether (PE) extract against the IVth instar larvae of Aedes aegypti recorded 100% mortality within 24 h of treatment compared to other solvent extracts. The aim of this study is to focus on the identification of the bioactive compounds present in the PE extract obtained from 72 h culture supernatant of Pseudomonas aeruginosa KUN2. Identification of the bio active compounds was done from the fractions collected using preparatory HPLC. Out of 18 fractions collected, fraction 3 showed 100 per cent mortality within 24 h of treatment. Further, fraction 3 was analysed using LCMS, NMR, and FTIR. The results revealed the presence of two major compounds such as 2-(dec-2-enyl)-3-methyl quinolin-4-ol-C20H27NO and 7-amino-N-methyl phenazine-1-carboxamide—C14 H13 N4O2 in fraction 3. Stability studies indicated that the crude PE extract and fraction 3 were stable at temperatures 30 ºC and 50 ºC. In vitro studies of fraction 3 using HDFa cell line recorded maximum of 7% inhibition at 50 µg/ml concentration.
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The identified compounds can be used further for the development of drug (Still research is ongoing).
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Acknowledgements
We would like to thank Department of Biotechnology, Karunya Institute of Technology and Sciences. Many thanks are extended to Dr. K.P. Salin, Department of Nematology, Sugar Cane Breeding Institute for all the supports.
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Lalithambika, B., Chandrapragasam, V., Mathew, J. et al. 2-(dec-2-enyl)-3-methyl quinolin-4-ol-C20H27NO and 7-amino-N-methyl phenazine-1-carboxamide—C14 H13 N4O2: potent bio-active compounds against dengue vector Aedes aegypti. Int J Trop Insect Sci 43, 703–718 (2023). https://doi.org/10.1007/s42690-023-00976-x
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DOI: https://doi.org/10.1007/s42690-023-00976-x