Skip to main content
Log in

Tools and techniques in forensic entomology- A critical review

  • Mini-review
  • Published:
International Journal of Tropical Insect Science Aims and scope Submit manuscript

Abstract

In forensic entomology, the presence of insects aids investigations by helping to understand the time since death. Insects are the first organisms that colonize and decompose the carrion. The main application of insect evidence is determining minimum post mortem interval (PMImin) either by analyzing insect developmental patterns or by estimating insect succession on the corpse. Age determination of insects has a pivotal role in the estimation of PMI. A range of techniques is available for estimating age, which provides accurate PMI in investigations. The present review critically discusses the applications of entomological evidence in PMI estimation and enlightens the factors influencing PMI calculations. Age estimation of insects is the primary task in PMI estimation. This review concentrates on methods used in age estimation, their strengths and weaknesses. Entomotoxicology analysis is a valuable tool in solving poisoning cases when dead bodies are recovered after a long period and this review also analyses the scope of entomotoxicology in the area of death investigations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Adams ZJ, Hall MJ (2003) Methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length. Forensic Sci Int 138(1–3):50–61

    Article  PubMed  Google Scholar 

  • Amendt J, Krettek R, Zehner R (2004) Forensic entomology. Naturwissenschaften 91(2):51–65

    Article  CAS  PubMed  Google Scholar 

  • Amendt J, Richards CS, Campobasso CP, Zehner R, Hall MJ (2011) Forensic entomology: applications and limitations. Forensic Sci Med Pathol 7(4):379–392

    Article  CAS  PubMed  Google Scholar 

  • Anderson GS (1999) Wildlife forensic entomology: determining time of death in two illegally killed black bear cubs. J Forensic Sci 44(4):856–859

    Article  CAS  PubMed  Google Scholar 

  • Anderson GS, Byrd J, Castner J (2001) Insect succession on carrion and its relationship to determining time of death. Forensic entomology: the utility of arthropods in legal investigations 143:76

    Google Scholar 

  • Bala M, Sharma A (2016) Review of some recent techniques of age determination of blow flies having forensic implications. Egypt J Forensic Sci 6(3):203–208

    Article  Google Scholar 

  • Barton PS, Cunningham SA, Lindenmayer DB, Manning AD (2013) The role of carrion in maintaining biodiversity and ecological processes in terrestrial ecosystems. Oecologia 171(4):761–772

    Article  PubMed  Google Scholar 

  • Boehme P, Spahn P, Amendt J, Zehner R (2013) Differential gene expression during metamorphosis: a promising approach for age estimation of forensically important Calliphora vicina pupae (Diptera: Calliphoridae). Int J Legal Med 127(1):243–249

    Article  PubMed  Google Scholar 

  • Bornemissza G (1957) An analysis of Arthropod succession in Carrion and the effect of its decomposiion on the soil fauna. Australian J Zool 5(1):1–12

    Article  Google Scholar 

  • Briere J-F, Pracros P, Le Roux A-Y, Pierre J-s (1999) A novel rate model of temperature-dependent development for arthropods. Environ Entomol 28(1):22–29

    Article  Google Scholar 

  • Byrd JH, Butler JF (1997) Effects of temperature on Chrysomya rufifacies (Diptera: Calliphoridae) development. J Med Entomol 34(3):353–358

    Article  CAS  PubMed  Google Scholar 

  • Campobasso CP, Di Vella G, Introna F (2001) Factors affecting decomposition and Diptera colonization. Forensic Sci Int 120(1–2):18–27

    Article  CAS  PubMed  Google Scholar 

  • Campobasso CP, Introna F (2001) The forensic entomologist in the context of the forensic pathologist’s role. Forensic Sci Int 120(1–2):132–139

    Article  CAS  PubMed  Google Scholar 

  • Catts EP, Goff ML (1992) Forensic entomology in criminal investigations. Ann Rev Entomol 37(1):253–272

    Article  CAS  Google Scholar 

  • Catts EP, Haskell NH (1990) Entomology & death: a procedural guide. Forensic Entomology Assocs

  • Chophi R, Sharma S, Sharma S, Singh R (2019) Forensic entomotoxicology: current concepts, trends and challenges. J Forensic Leg Med 67:28–36

    Article  PubMed  Google Scholar 

  • de Moretti C, Giannotti T, Thyssen E, Solis PJ, Godoy WAC (2011) Bait and habitat preferences, and temporal variability of social wasps (Hymenoptera: Vespidae) attracted to vertebrate carrion. J Med Entomol 48(5):1069–1075

    Article  Google Scholar 

  • Dekeirsschieter J, Frederickx C, Verheggen F, Boxho P, Haubruge E (2013) Forensic entomology investigations from Doctor Marcel Leclercq (1924–2008): a review of cases from 1969 to 2005. J Med Entomol 50(5):935–954

    Article  CAS  PubMed  Google Scholar 

  • Frederickx C, Dekeirsschieter J, Brostaux Y, Wathelet J-P, Verheggen F, Haubruge E (2012) Volatile organic compounds released by blowfly larvae and pupae: New perspectives in forensic entomology. Forensic Sci Int 219(1–3):215–220

    Article  CAS  PubMed  Google Scholar 

  • Fuller ME (1934) The insect inhabitants of carrion: a study in animal ecology. Bulletin of the Council for Scientific and Industrial Research, Australia( 82)

  • Gaudry E (2009) The insects colonisation of buried remains. Current concepts in forensic entomology. Springer, pp 273–311

  • Gaudry E, Blais C, Maria A, Dauphin-Villemant C (2006) Study of steroidogenesis in pupae of the forensically important blow fly Protophormia terraenovae (Robineau-Desvoidy)(Diptera: Calliphoridae). Forensic Sci Int 160(1):27–34

    Article  CAS  PubMed  Google Scholar 

  • Giao JZ, Godoy WAC (2007) Ovipositional behavior in predator and prey blowflies. J insect Behav 20(1):77–86

    Article  Google Scholar 

  • Glaister J, Brash JC (1937) Medico-legal aspects of the Ruxton case. W. Wood

  • Goff ML (1993) Estimation of postmortem interval using arthropod development and successional patterns. Forensic Sci Rev 5:81–81

    CAS  PubMed  Google Scholar 

  • Greenberg B (1991) Flies as forensic indicators. J Med Entomol 28(5):565–577

    Article  CAS  PubMed  Google Scholar 

  • Greenberg B, Kunich JC (2002) Entomology and the law: flies as forensic indicators. Cambridge University Press

  • Hall D, Gerhardt R (2002) Flies (Diptera); in medical and veterinary entomology. Mullen G. L. Academic Press, San Diego, Durden

    Google Scholar 

  • Hall RD, Huntington T (2001) Introduction: perceptions and status of forensic entomology. Forensic entomology: the utility of arthropods in legal investigations,1–15

  • Harvey ML, Gasz NE, Voss SC (2016) Entomology-based methods for estimation of postmortem interval. Res Rep forensic Med Sci 6:1–9

    Google Scholar 

  • Illingworth J Insects attracted to carrion in Southern California. In Proc Hawaii Entomol Soc, 1926 (Vol. 6, pp. 397–401)

  • Introna F, Dico CL, Caplan YH, Smialek JE (1990) Opiate analysis in cadaveric blowfly larvae as an indicator of narcotic intoxication. J Forensic Sci 35(1):118–122

    Article  PubMed  Google Scholar 

  • Jaques HE (1915) The fish-feeding coleoptera of Cedar Point

  • Johnston W, Villeneuve G (1897) On the medico-legal application of entomology, vol 45315. sn, Sl

    Google Scholar 

  • Karampela S, Pistos C, Moraitis K, Stoukas V, Papoutsis I, Zorba E et al (2015) Development and validation of a LC/MS method for the determination of ∆9-tetrahydrocannabinol and 11-carboxy-∆9-tetrahydrocannabinol in the larvae of the blowfly Lucilia sericata: Forensic applications. Sci Justice 55(6):472–480

    Article  PubMed  Google Scholar 

  • Kashyap V, Pillay V (1989) Efficacy of entomological method in estimation of postmortem interval: A comparative analysis. Forensic Sci Int 40(3):245–250

    Article  CAS  PubMed  Google Scholar 

  • Ko C (2002) Cases in the history of Chinese trials [English translation of Zhe yu gui jian bu] Lu Shih China. Cambridge University Press, Cambridge

    Google Scholar 

  • Kulshrestha P, Chandra H (1987) Time since death: an entomological study on corpses. Am J Forensic Med Pathol 8(3):233–238

    Article  CAS  PubMed  Google Scholar 

  • Leclercq J (2008) Marcel Leclercq (1924–2008), médecin, diptériste, parasitologue et pionnier de l’entomologie forensique (Part. 1). Entomologie faunistique-Faunistic Entomology

  • Leclercq M (1969) Entomological parasitology. The relations between entomology and the medical sciences. Entomological parasitology. The relations between entomology and the medical sciences.

  • Leclercq M (1978) Entomologie et médecine légale. Datation de la mort (Vol. 614.1 L459e): París, FR: Masson

  • Leclercq M, Quinet L (1949) Quelques cas d’application de l’entomologie a la deÂtermination de l’eÂpoque de mort Several cases concerning the application of entomology on determination of postmortem interval. Ann Med LeÂg 29:324–326

    Google Scholar 

  • Lehane M, Chadwick J, Howe M, Mail T (1986) Improvements in the pteridine method for determining age in adult male and female Stomoxys calcitrans (Diptera: Muscidae). J Econ Entomol 79(6):1714–1719

    Article  CAS  Google Scholar 

  • Lockey KH (1988) Lipids of the insect cuticle: origin, composition and function. Comp Biochem Physiol Part B: Comp Biochem 89(4):595–645

    Article  Google Scholar 

  • Lord W, Stevenson J (1986) American registered professional entomologists. Washington DC: Chesapeake,42

  • Mail T, Chadwick J, Lehane M (1983) Determining the age of adults of Stomoxys calcitrans (L.)(Diptera: Muscidae). Bull Entomol Res 73(3):501–525

    Article  Google Scholar 

  • Mashaly AMA (2017) Carrion beetles succession in three different habitats in Riyadh, Saudi Arabia. Saudi J Biol Sci 24(2):430–435

    Article  PubMed  Google Scholar 

  • McKnight B (1981) the washing away of wrongs: forensic medicine in thirteenthcentury China. University of Michigan, Ann Arbor

    Google Scholar 

  • Mégnin J-P (1887) La faune des tombeaux. Gauthier-Villars

  • Mégnin P (1894) La faune des cadavres: application de l’entomologie à la médecine légale. Gauthier-Villars

  • Motter MG (1898) A contribution to the study of the fauna of the grave. A study of on hundred and fifty disinterments, with some additional experimental observations. J New York Entomol Soc 6(4):201–231

    Google Scholar 

  • Niezabitowski E (1902) Experimentelle Beitrage zur Lehre von der Leichenfauna. Vjschr fg M 23:44–50

    Google Scholar 

  • Nolte KB, Pinder RD, Lord W (1992) Insect larvae used to detect cocaine poisoning in a decomposed body. J Forensic Sci 37(4):1179–1185

    Article  CAS  PubMed  Google Scholar 

  • Owings CG, Spiegelman C, Tarone AM, Tomberlin JK (2014) Developmental variation among Cochliomyia macellaria Fabricius (Diptera: Calliphoridae) populations from three ecoregions of Texas, USA. Int J Legal Med 128(4):709–717

    Article  PubMed  Google Scholar 

  • Parmenter RR, MacMahon JA (2009) Carrion decomposition and nutrient cycling in a semiarid shrub–steppe ecosystem. Ecol Monogr 79(4):637–661

    Article  Google Scholar 

  • Payne JA (1965) A summer carrion study of the baby pig Sus scrofa Linnaeus. Ecology 46(5):592–602

    Article  Google Scholar 

  • Payne JA, King EW, Beinhart G (1968) Arthropod succession and decomposition of buried pigs. Nature 219(5159):1180–1181

    Article  CAS  PubMed  Google Scholar 

  • Pechal JL, Benbow ME, Crippen TL, Tarone AM, Tomberlin JK (2014) Delayed insect access alters carrion decomposition and necrophagous insect community assembly. Ecosphere 5(4):1–21

    Article  Google Scholar 

  • Pien K, Laloup M, Pipeleers-Marichal M, Grootaert P, De Boeck G, Samyn N et al (2004) Toxicological data and growth characteristics of single post-feeding larvae and puparia of Calliphora vicina (Diptera: Calliphoridae) obtained from a controlled nordiazepam study. Int J Legal Med 118(4):190–193

    Article  PubMed  Google Scholar 

  • Ratte HT (1984) Temperature and insect development. Environmental physiology and biochemistry of insects. Springer, pp 33–66

  • Reed H Jr (1958) A study of dog carcass communities in Tennessee, with special reference to the insects.American midland naturalist,213–245

  • Rivers D, Dahlem G (2014) Biology, taxonomy, and natural history of forensically important insects. West Sussex, UK, Wiley & Sons, Ltd., pp 69–94

  • Rivers DB, Dahlem GA (2014) The science of forensic entomology. John Wiley & Sons

  • Sadler D, Fuke C, Court F, Pounder D (1995) Drug accumulation and elimination in Calliphora vicina larvae. Forensic Sci Int 71(3):191–197

    Article  CAS  PubMed  Google Scholar 

  • Sawyer SJ (2017) Effects of Carrion Decomposition. on Arthropod Community Structure and Habitat Seeking Behavior

  • Sharma R, Garg RK, Gaur J (2015) Various methods for the estimation of the post mortem interval from Calliphoridae: A review. Egypt J Forensic Sci 5(1):1–12

    Article  CAS  Google Scholar 

  • Shirley NR, Wilson RJ, Jantz LM (2011) Cadaver use at the University of Tennessee’s anthropological research facility. Clin Anat 24(3):372–380

    Article  PubMed  Google Scholar 

  • Smith S (1951) History and development of forensic medicine. BMJ 1(4707):599

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tarone AM, Jennings KC, Foran DR (2007) Aging blow fly eggs using gene expression: a feasibility study. J Forensic Sci 52(6):1350–1354

    Article  CAS  PubMed  Google Scholar 

  • Tomberlin JK, Benbow ME, Tarone AM, Mohr RM (2011) Basic research in evolution and ecology enhances forensics. Trends Ecol Evol 26(2):53–55

    Article  PubMed  Google Scholar 

  • Verón IF, Zorrilla I, Richards NL (2021) Is deliberate pesticide poisoning of wildlife impacting local insect communities? Wildlife and environmental forensic investigations in southern Spain present an opportunity for collaborative entomological monitoring.Journal of Insect Conservation,1–9

  • Wells JD, Stevens J, Byrd J, Castner J (2009) Molecular methods for forensic entomology. JH Castner and JL Byrd (éds.), Forensic Entomology: the Utility of Arthropods in Legal Investigations, 2, 437–452

  • Wilson EE, Wolkovich EM (2011) Scavenging: how carnivores and carrion structure communities. Trends Ecol Evol 26(3):129–135

    Article  PubMed  Google Scholar 

  • Wolff M, Uribe A, Ortiz A, Duque P (2001) A preliminary study of forensic entomology in Medellın, Colombia. Forensic Sci Int 120(1–2):53–59

    Article  CAS  PubMed  Google Scholar 

  • Yovanovitch GP (1888) Entomologie appliquée à la médicine légale. Ollier-Henry

  • Zehner R, Amendt J, Schütt S, Sauer J, Krettek R, Povolný D (2004) Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae). Int J Legal Med 118(4):245–247

    Article  PubMed  Google Scholar 

Download references

Funding

No funds, grants, or other support was received.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Embalil Mathachan Aneesh.

Ethics declarations

Conflicts of interest/ competing interests

The authors have no conflicts of interest to declare that are relevant to the content of this article.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Siva Prasad, M.S., Aneesh, E.M. Tools and techniques in forensic entomology- A critical review. Int J Trop Insect Sci 42, 2785–2794 (2022). https://doi.org/10.1007/s42690-022-00823-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42690-022-00823-5

Keywords

Navigation