Abstract
A new method for preparing the males genitalia from the tribe Phycitini using the Slide Dryer BIS-N 209–1 is proposed. The recommended time interval for exposition in KOH solution is from 30 to 50 min with a fixed temperature of 80 °C. It is shown that the degree of light transmittance of the genitalia depends on time in an exponential way. The light transmission coefficients for tegumen and gnathos were determined by exponential fitting of the experimental data, they were 24 ± 7 and 37 ± 12 min, respectively. The genitalia reach the maximum degree of enlightenment after 80 min, the brightness of the sclerites practically does not change with further impact to KOH solution.
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Data availability
The primary data is available in a repository (Lovtsova and Kochiev, 2020).
References
Clarke JFG (1941) The preparation of slides of the genitalia of Lepidoptera. Bullet Brooklyn Entomol Soc 36:149–161
Dias FMS, Casagrande M, Mielke OHH (2010) Alternative techniques to study characters of the genitalia in Lepidoptera Neotropical Entomology. 39(6):1044-1045
Hardwick DF (1950) Preparation of slide mounts of Lepidopterous genitalia. Can Entomol 10:231–235. https://doi.org/10.4039/Ent82231-11
Lee SM, Brown RL (2006) A new method for preparing slide mounts of whole bodies of Microlepidoptera. J Asia-Pacific Entomol 9(3):249–253
Lovtsova J, Kochiev M (2020) The possibility of using a Slide Dryer BIS-N 09–1 for the preparation of male genitalia of tribe Phycitini. Mendeley Data V1. https://doi.org/10.17632/dkt4yy3f7s.1
Padwal KG, Sharma SK, Singh SK (2018) Dissection and slide mounting technique for male and female genitalia of Leucinodes orbonalis. J Entomol Res 42(2):259–262. https://doi.org/10.5958/0974-4576.2018.00043.9
Robinson GS (1976) The preparation of slides of Lepidoptera genitalia with special reference to the Microlepidoptera. The Entomologist’s Gazette 27:127–132
Zagulajev AK (1965) Moths and Pyralid moths ‒ pests of grain and food supplies. Moscow. Leningrad. Nauka 1‒273
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Acknowledgements
The authors are sincerely grateful to Dr. Ilya O. Kamayev (All-Russian Plant Quarantine Center, Bykovo, Russia) for a gift of the material, Andrey V. Schipulin (All-Russian Plant Quarantine Center, Bykovo, Russia) for help with equipment.
Funding
This work was carried out at the All-Russian Plant Quarantine Center (State Research Program 5121042600339–5).
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Lovtsova, J.A., Kochiev, M.V. New method of the preparation of male genitalia of tribe Phycitini (Lepidoptera: Pyralidae) using a slide dryer. Int J Trop Insect Sci 42, 3185–3188 (2022). https://doi.org/10.1007/s42690-022-00818-2
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DOI: https://doi.org/10.1007/s42690-022-00818-2