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Synergistic effect of minerals solubilizing and siderophores producing bacteria as different microbial consortium for growth and nutrient uptake of oats (Avena sativa L.)

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Abstract

Indiscriminate use of chemical fertilizers increases agricultural productivity but on the other hand they harms the health of environment and humans. The multifunctional plant growth promoting bacterial strains could be an ideal alternative to synthetic chemical fertilizers. Looking into the benefits of plant growth promoting bacteria, the present study deals with the isolation of mineral solubilizing and Fe-chelating microbes from the rhizospheric regions of different crops growing in hilly regions of Baru Sahib. Isolated microbes were screened for the multifarious plant growth promoting attributes including solubilization of phosphorus, potassium, zinc, selenium; production of siderophores, ammonia, indole-3-acetic acid, hydrogen cyanide, and nitrogen fixation. The efficient mineral solubilizing and siderophores producing bacterial strains were identified as Serratia surfactantfaciens EU-C3SY2 (P-solubilizer; 144.5 ± 0.05 mg L− 1), Serratia marcescens EU-D1RNL1 (K- solubilizers; 46.9 ± 1.2 mg mL− 1), Serratia nematodiphila EU-D2SRY4 (Zn-solubilizer; 68.5 ± 0.03 mg L− 1), Erwinia persicina EU-B1RT3.1 (Se-solubilizer) and Serratia sp. EU-C1RK1 (siderophores producer; 69.16 ± 0.71 psu) using 16S rRNA gene sequencing. The selected efficient bacterial strains were used for the development of six different microbial consortia, which were evaluated for the growth promotion of oats crops under green house and open field conditions. Among the six different microbial combination, the consortium developed from EU-C3SY2, EU-D1RNL1, EU-D2SRY4, EU-B1RT3.1, and EU-C1RK1 were found as most effective. This microbial combination significantly increased the growth and physiological parameters of oats plants as compared to other developed microbial consortia, control, and agro-chemical fertilizers. The developed mineral solubilizing and Fe-chelating microbial consortium could be used as biofertilizers for crops growing in the hilly regions for agricultural sustainability.

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References

  • Anderson K (2010) Globalization’s effects on world agricultural trade, 1960–2050. Philos Trans R Soc B 365:3007–3021

    Article  Google Scholar 

  • Anttila H, Sontag-Strohm T, Salovaara H (2004) Viscosity of beta-glucan in oat products. Agr Food Sci 13:80–87

    Article  CAS  Google Scholar 

  • Arruda L, Beneduzi A, Martins A, Lisboa B, Lopes C, Bertolo F et al (2013) Screening of rhizobacteria isolated from maize (Zea mays L.) in Rio Grande do Sul State (South Brazil) and analysis of their potential to improve plant growth. Appl Soil Ecol 63:15–22

    Article  Google Scholar 

  • Asaf S, Khan MA, Khan AL, Waqas M, Shahzad R, Kim A-Y et al (2017) Bacterial endophytes from arid land plants regulate endogenous hormone content and promote growth in crop plants: an example of Sphingomonas sp. and Serratia marcescens. J Plant Interact 12:31–38

    Article  CAS  Google Scholar 

  • Asghari B, Khademian R, Sedaghati B (2020) Plant growth promoting rhizobacteria (PGPR) confer drought resistance and stimulate biosynthesis of secondary metabolites in pennyroyal (Mentha pulegium L.) under water shortage condition. Sci Hortic 263:109132

    Article  CAS  Google Scholar 

  • Bakker AW, Schippers B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp-mediated plant growth-stimulation. Soil Biol Biochem 19:451–457

    Article  CAS  Google Scholar 

  • Bose A, Shah D, Keharia H (2013) Production of indole-3-acetic-acid (IAA) by the white rot fungus Pleurotus ostreatus under submerged condition of Jatropha seedcake. Mycology 4:103–111

    Article  CAS  Google Scholar 

  • Campos EV, Proença PL, Oliveira JL, Bakshi M, Abhilash P, Fraceto LF (2019) Use of botanical insecticides for sustainable agriculture: future perspectives. Ecol Indic 105:483–495

    Article  CAS  Google Scholar 

  • Cappucino J, Sherman N (1992) Nitrogen cycle. Microbiology: a laboratory manual. Benjamin/Cumming Pub. Co., New York

    Google Scholar 

  • Clewer AG, Scarisbrick DH (2013) Practical statistics and experimental design for plant and crop science. Wiley

  • Devi R, Kaur T, Kour D, Yadav A, Yadav AN, Suman A et al (2022a) Minerals solubilizing and mobilizing microbiomes: a sustainable approaches for managing minerals deficiency in agricultural soil. J Appl Microbiol 133(3):1245–1272

    Article  PubMed  Google Scholar 

  • Devi R, Kaur T, Kour D, Yadav AN, Suman A (2022b) Potential applications of mineral solubilizing rhizospheric and nitrogen fixing endophytic bacteria as microbial consortium for the growth promotion of Chilli (Capsicum annum L). Biologia 77:2933–2943

    Article  CAS  Google Scholar 

  • Devi R, Alsaffar MF, Al-Taey DKA, Kumar S, Negi R, Sharma B et al (2024) Effect of indigenous mineral availing microbial consortia and cattle manure combination for growth of maize (Zea mays L). https://doi.org/10.1007/s42535-024-00897-1. Vegetos

  • Fasim F, Ahmed N, Parsons R, Gadd GM (2002) Solubilization of zinc salts by a bacterium isolated from the air environment of a tannery. FEMS Microbiol Lett 213:1–6

    Article  CAS  PubMed  Google Scholar 

  • Francis AJ, Dodge C, Chendrayan K, Quinby HL (1988) Anaerobic microbial dissolution of lead and production of organic acids. US patent No. 4758345

  • González A, Tello J, Cara M (2005) First report of Erwinia persicina from Phaseolus vulgaris in Spain. Plant Dis 89:109–109

    Article  PubMed  Google Scholar 

  • Gupta S, Pandey S (2020) Enhanced salinity tolerance in the common bean (Phaseolus vulgaris) plants using twin ACC deaminase producing rhizobacterial inoculation. Rhizosphere 16:100241

    Article  Google Scholar 

  • Han HS, Lee K (2006) Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant Soil Environ 52:130–136

    Article  CAS  Google Scholar 

  • Han SO, New PB (1998) Variation in nitrogen fixing ability among natural isolates of Azospirillum. Microb Ecol 36:193–201

    Article  CAS  PubMed  Google Scholar 

  • Herranen M, Kariluoto S, Edelmann M, Piironen V, Ahvenniemi K, Iivonen V et al (2010) Isolation and characterization of folate-producing bacteria from oat bran and rye flakes. Int J Food Microbiol 142:277–285

    Article  CAS  PubMed  Google Scholar 

  • Holland J, White P, Glendining M, Goulding K, McGrath S (2019) Yield responses of arable crops to liming–An evaluation of relationships between yields and soil pH from a long-term liming experiment. Euro J Agron 105:176–188

    Article  CAS  Google Scholar 

  • Hu X, Chen J, Guo J (2006) Two phosphate-and potassium-solubilizing bacteria isolated from Tianmu Mountain, Zhejiang, China. World J Microbiol Biotechnol 22:983–990

    Article  CAS  Google Scholar 

  • Irigoyen J, Einerich D, Sánchez-Díaz M (1992) Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago Sativd) plants. Physiol Plant 84:55–60

    Article  CAS  Google Scholar 

  • Joshi D, Kumar S, Suyal DC, Goel R (2017) The microbiome of the himalayan ecosystem. In: Kalia VC, Shouche Y, Purohit HJ, Rahi P (eds) Mining of Microbial Wealth and MetaGenomics. Springer Singapore, Singapore, pp 101–116

    Chapter  Google Scholar 

  • Kaur T, Devi R, Kumar S, Kour D, Yadav AN (2022a) Plant growth promotion of pearl millet (Pennisetum glaucum L.) by novel bacterial consortium with multifunctional attributes. Biologia 78:621–631

    Article  Google Scholar 

  • Kaur T, Devi R, Kumar S, Kour D, Yadav AN (2022b) Synergistic effect of Endophytic and Rhizospheric Microbes for Plant Growth Promotion of Foxtail Millet (Setaria italica L). Natl Acad Sci Lett 46:27–30

    Article  Google Scholar 

  • Kaur T, Devi R, Kumar S, Sheikh I, Kour D, Yadav AN (2022c) Microbial consortium with nitrogen fixing and mineral solubilizing attributes for growth of barley (Hordeum vulgare L). Heliyon 8:e09326

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kaur T, Devi R, Negi R, Kour D, Yadav AN (2023) Mutualistic effect of macronutrients availing microbes on the plant growth promotion of finger millet (Eleusine coracana L). Curr Microbiol 80:186

    Article  CAS  PubMed  Google Scholar 

  • Kawaguchi A, Abe D, Saito T, Nogata Y, Nomiyama K, Kohyama N et al (2021) Pink seed of barley caused by Erwinia persicina. J Gen Plant Pathol 87:106–109

    Article  Google Scholar 

  • Kawalekar JS (2013) Role of biofertilizers and biopesticides for sustainable agriculture. J Bio Innov 2:73–78

    Google Scholar 

  • Kim DO, Jeong SW, Lee CY (2003) Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 81:321–326

    Article  CAS  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophyll fluorescence signatures of leaves during the autumnal chlorophyll breakdown. J Plant Physiol 131:101–110

    Article  CAS  Google Scholar 

  • Mishra P, Dash D (2014) Rejuvenation of biofertilizer for sustainable agriculture and economic development. Consilience 11:41–61

    Google Scholar 

  • Mohamed EA, Farag AG, Youssef SA (2018) Phosphate solubilization by Bacillus subtilis and Serratia marcescens isolated from tomato plant rhizosphere. J Environ Prot 9:83367

    Article  Google Scholar 

  • Monib M, Zahra M, Abdel el-Al S, Heggo A (1984)Role of silicate bacteria in releasing K and Si from biotite and orthoclase. In: Szegi J (ed) Soil biology and conservation of the biosphere. Akademiai Kiado, Budapest, pp 733–743

    Google Scholar 

  • Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal chim Acta 27:31–36

    Article  CAS  Google Scholar 

  • Palmieri D, Vitullo D, De Curtis F, Lima G (2017) A microbial consortium in the rhizosphere as a new biocontrol approach against fusarium decline of chickpea. Plant Soil 412:425–439

    Article  CAS  Google Scholar 

  • Park HJ, Cha HC (2003) Flavonoids from leaves and exocarps of the grape Kyoho. Korean J Biol Sci 7:327–330

    Article  CAS  Google Scholar 

  • Patten CL, Glick BR (2002) Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl Environ Microbiol 68:3795–3801

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pikovskaya R (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17:362–370

    CAS  Google Scholar 

  • Raghuwanshi R (2012) Opportunities and challenges to sustainable agriculture in India. Nebio 3:78–86

    Google Scholar 

  • Ruanchaiman S, Kumsopa A, Boontanon N, Prapagdee B (2009) Dispersion of cadmium-resistant bacteria in cadmium-contaminated soils at Mae Sot District, Tak province. Appl Environ Res 31:35–48

    Google Scholar 

  • Schwyn B, Neilands J (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56

    Article  CAS  PubMed  Google Scholar 

  • Stres B, Sul WJ, Murovec B, Tiedje JM (2013) Recently deglaciated high-altitude soils of the Himalaya: diverse environments, heterogenous bacterial communities and long-range dust inputs from the upper Troposphere. PLoS ONE 8:e76440

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Su C, Xiang Z, Liu Y, Zhao X, Sun Y, Li Z et al (2016) Analysis of the genomic sequences and metabolites of Serratia surfactantfaciens sp. nov. YD25 T that simultaneously produces prodigiosin and serrawettin W2. BMC Genom 17:1–19

    Article  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Tripura C, Sashidhar B, Podile AR (2007) Ethyl methanesulfonate mutagenesis–enhanced mineral phosphate solubilization by groundnut-associated Serratia marcescens GPS-5. Curr Microbiol 54:79–84

    Article  CAS  PubMed  Google Scholar 

  • Umesha S, Manukumar HM, Chandrasekhar B (2018) Sustainable agriculture and food security. In: Singh RL, Mondal S (eds) Biotechnology for sustainable agriculture. Woodhead Publishing, pp 67–92

  • Uosif M, Mostafa A, Elsaman R, Moustafa E-S (2014) Natural radioactivity levels and radiological hazards indices of chemical fertilizers commonly used in Upper Egypt. J Radiat Res Appl Sci 7:430–437

    Google Scholar 

  • Venieraki A, Dimou M, Pergalis P, Kefalogianni I, Chatzipavlidis I, Katinakis P (2011) The genetic diversity of culturable nitrogen-fixing bacteria in the rhizosphere of wheat. Microb Ecol 61:277–285

    Article  PubMed  Google Scholar 

  • Verma P, Yadav AN, Kazy SK, Saxena AK, Suman A (2014) Evaluating the diversity and phylogeny of plant growth promoting bacteria associated with wheat (Triticum aestivum) growing in central zone of India. Int J Curr Microbiol Appl Sci 3:432–447

    Google Scholar 

  • Verma P, Yadav AN, Khannam KS, Kumar S, Saxena AK, Suman A (2016) Molecular diversity and multifarious plant growth promoting attributes of Bacilli associated with wheat (Triticum aestivum L.) rhizosphere from six diverse agro-ecological zones of India. J Basic Microbiol 56:44–58

    Article  CAS  PubMed  Google Scholar 

  • Wang HY, Shen L, Zhai LM, Zhang JZ, Ren TZ, Fan BQ et al (2015) Preparation and utilization of phosphate biofertilizers using agricultural waste. J Integr Agric 14:158–167

    Article  CAS  Google Scholar 

  • Yadav AN, Sachan SG, Verma P, Saxena AK (2016) Bioprospecting of plant growth promoting psychrotrophic Bacilli from cold desert of north western Indian Himalayas. Indian J Exp Biol 54:142–150

    PubMed  Google Scholar 

  • Youssef M, Eissa M (2014) Biofertilizers and their role in management of plant parasitic nematodes. A review. J Biotechnol Pharm Res 5:1–6

    Google Scholar 

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Acknowledgements

The authors are grateful to the Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib and Department of Environment, Science & Technology (DEST), Shimla, HP funded project “Development of microbial consortium as bio-inoculants for drought and low temperature growing crops for organic farming in Himachal Pradesh” for providing the facilities support, to undertake the investigations.

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Author notes

  1. Rubee Devi and Tanvir Kaur contributed equally in the present work.

Authors and Affiliations

  1. Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, Himachal Pradesh, India

    Rubee Devi, Rajeshwari Negi, Tanvir Kaur & Ajar Nath Yadav

  2. Medical Laboratories Techniques Department, AL- Mustaqbal University College, Hillah, Babil, 51001, Iraq

    Marwa Fadhil Alsaffar

  3. Department of Horticulture, AL- Qasim Green University, Babylon, Iraq

    Duraid K.A. AL-Taey

  4. Department of Genetics and Plant Breeding, Faculty of Agricultural Science, GLA university, Mathura, India

    Sanjeev Kumar

  5. Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour, Himachal Pradesh, India

    Babita Sharma & Divjot Kour

  6. Department of Food Technology, School of Applied and Life sciences, Uttaranchal University, Dehradun, Uttarakhand, India

    Sarvesh Rustagi

  7. Department of Botany, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour, 173101, India

    Amrik Singh Ahluwalia

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  1. Rubee Devi
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Contributions

RD, TK and RN carried out the experimental part and wrote the manuscript; DK and figures and tables; MFA, DKA, SK and KKC reviewing the manuscript and ANY give the hypothesis and idea of research work.

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Correspondence to Ajar Nath Yadav.

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Devi, R., Alsaffar, M.F., AL-Taey, D.K. et al. Synergistic effect of minerals solubilizing and siderophores producing bacteria as different microbial consortium for growth and nutrient uptake of oats (Avena sativa L.). Vegetos (2024). https://doi.org/10.1007/s42535-024-00922-3

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