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Evaluation and characterization of EMS induced mutant population of Gossypium herbaceum

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Abstract

Gossypium herbaceum is reported to have innate adaptability to withstand biotic and abiotic stresses. However, the continued selection for desired traits eliminates the rare alleles from advanced cultivars and makes the species vulnerable to several stresses. The induced mutagenesis expands the genetic basis of the existing germplasm. In the present study, 5500 seeds of G. herbaceum (cv. WAGAD) were treated by 70 mM ethyl methane sulfonate (EMS), established 2597 fertile M1 plants, which were used in development of 6600 M2 plants followed by 5473 M3 plants. About 5% of the plants showed visible mutant phenotypes like sterility, chlorosis, altered leaf morphology and early flowering/late flowering. The phenotypic evaluation of 4453 plants for 11 agro-morphological traits revealed significant variations. Random amplified polymorphic DNA (RAPD) profiling of a random set of 150 M3 plants revealed the considerable level of mutation in the genome. The developed mutant population could serve as an important genetic resource for forward and reverse genetic studies. Further, the potential mutant plants could also be used for pyramiding targeted traits and consequently for developing new high yielding varieties.

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Acknowledgements

The authors thank to the director CSIR-NBRI and MGM for providing the facilities to carry out the present study. UK thanks to CSIR for the Fellowship (Junior Research Fellowship & Senior Research Fellowship).

Funding

The present study was funded by Council of Scientific & Industrial Research (CSIR) through a project “Genomics of medicinal plants and agronomically important traits (PlaGen)” (BSC 0107). The institutional assigned manuscript number is “CSIR-NBRI_MS/2020/06/03”.

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Authors and Affiliations

  1. Plant Genetic Resources and Improvement, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India

    Umesh Kumar & Hemant Kumar Yadav

  2. Genetics and Molecular Biology Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India

    Samir V. Sawant

  3. Agricultural Biotechnology, Mahatma Gandhi Mission College, Aurangabad, Maharashtra, India

    Nilesh S. Chavan

  4. Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, India

    Umesh Kumar, Samir V. Sawant & Hemant Kumar Yadav

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  1. Umesh Kumar
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Kumar, U., Chavan, N.S., Sawant, S.V. et al. Evaluation and characterization of EMS induced mutant population of Gossypium herbaceum. Vegetos 35, 1036–1046 (2022). https://doi.org/10.1007/s42535-022-00385-4

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