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Nodulation in the absence of nod genes induction: alternative mechanisms involved in the symbiotic interaction between Cupriavidus sp. UYMMa02A and Mimosa pudica

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Abstract

Cupriavidus sp. UYMMa02A is a beta-rhizobia strain of the Cupriavidus genus, isolated from nodules of Mimosa magentea in Uruguay. This strain can form effective nodules with several Mimosa species, including its original host. Genome analyses indicate that Cupriavidus sp. UYMMa02A has a highly conserved 35 kb symbiotic island containing nod, nif, and fix operons, suggesting conserved mechanisms for the symbiotic interaction with plant hosts. However, while Cupriavidus sp. UYMMa02A produces functional nodules and promotes Mimosa pudica growth under nitrogen-limiting conditions, nod genes are not induced by luteolin or exposure to Mimosa spp. root exudate. To explore alternative mechanisms implicated in the Cupriavidus-Mimosa interaction, we assessed the proteomic profiles of Cupriavidus sp. UYMMa02A grown in the presence of pure flavonoids and co-culture with M. pudica plants. This approach allowed us to identify 24 differentially expressed proteins potentially involved in bacterial-plant interaction. In light of the obtained results, a possible model for nod-alternative symbiotic interaction is proposed.

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Acknowledgement

The authors would like to thank to Prof. Catherine Masson-Boivin for providing plasmids pCZ388 and pCBM01.

Funding

Funding was provided by Agencia Nacional de Investigación e Innovación (grant no. FCE_1_2014_1_104338, FCE_1_2017_1_136082, FCE_1_2019_1_156520), Programa de desarrollo de las ciencias básicas, PEDECIBA (grant no. 2018), and FONTAGRO (grant no. ID 30).

Author information

Author notes

  1. Carolina Croci

    Present address: Laboratorio de Ecología Microbiana Acuática, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Montevideo, Uruguay

  2. Cecilia Rodríguez-Esperón and Laura Sandes contributed equally to the present work.

Authors and Affiliations

  1. Laboratorio de Microbiología Ambiental. Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Montevideo, Uruguay

    Cecilia Rodríguez-Esperón, Laura Sandes, Ignacio Eastman, Carolina Croci, Florencia Garabato, Virginia Ferreira & Raúl A. Platero

  2. OXIProteomics, Créteil, France

    Martín Baraibar

  3. Unidad Mixta de Bioquímica y Proteómica Analíticas, Institut Pasteur de Montevideo-Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Montevideo, Uruguay

    Magdalena Portela & Rosario Durán

  4. Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay

    Magdalena Portela

Authors
  1. Cecilia Rodríguez-Esperón
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  2. Laura Sandes
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  3. Ignacio Eastman
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  4. Carolina Croci
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  5. Florencia Garabato
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  6. Virginia Ferreira
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  7. Martín Baraibar
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  8. Magdalena Portela
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  9. Rosario Durán
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  10. Raúl A. Platero
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Corresponding author

Correspondence to Raúl A. Platero.

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Rodríguez-Esperón, C., Sandes, L., Eastman, I. et al. Nodulation in the absence of nod genes induction: alternative mechanisms involved in the symbiotic interaction between Cupriavidus sp. UYMMa02A and Mimosa pudica. Environmental Sustainability 6, 383–401 (2023). https://doi.org/10.1007/s42398-023-00286-5

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