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Functional analysis of the two cyclophilin isoforms of Sinorhizobium meliloti

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Abstract

The nitrogen fixing Sinorhizobium meliloti possesses two genes, ppiA and ppiB, encoding two cyclophilin isoforms which belong to the superfamily of peptidyl prolyl cis/trans isomerases (PPIase, EC: 5.2.1.8). Here, we functionally characterize the two proteins and we demonstrate that both recombinant cyclophilins are able to isomerise the Suc-AAPF-pNA synthetic peptide but neither of them displays chaperone function in the citrate synthase thermal aggregation assay. Furthermore, we observe that the expression of both enzymes increases the viability of E. coli BL21 in the presence of abiotic stress conditions such as increased heat and salt concentration. Our results support and strengthen previous high-throughput studies implicating S. meliloti cyclophilins in various stress conditions.

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Authors and Affiliations

  1. Laboratory of General and Agricultural Microbiology, Faculty of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Eirini-Evangelia Thomloudi, Aggeliki Skagia, Anastasia Venieraki, Panagiotis Katinakis & Maria Dimou

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  1. Eirini-Evangelia Thomloudi
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  2. Aggeliki Skagia
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  3. Anastasia Venieraki
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  4. Panagiotis Katinakis
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  5. Maria Dimou
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Correspondence to Maria Dimou.

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Thomloudi, EE., Skagia, A., Venieraki, A. et al. Functional analysis of the two cyclophilin isoforms of Sinorhizobium meliloti . World J Microbiol Biotechnol 33, 28 (2017). https://doi.org/10.1007/s11274-016-2201-6

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  • DOI: https://doi.org/10.1007/s11274-016-2201-6

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