Skip to main content
SpringerLink
Log in

Molecular characterization of sequence variants of Chrysanthemum chlorotic mottle viroid from Himachal Pradesh, India

  • New Reports
  • Published:
Indian Phytopathology Aims and scope Submit manuscript

Abstract

Chrysanthemum is an important cut flower crop but is known to be infected with viruses and viroids. Here, we have identified and characterized two sequence variants of Chrysanthemum chlorotic mottle viroid (CCHMVd) from chrysanthemum varieties Yellow Star and Orange Daisy that showed stunting, mottling and chlorosis-like symptoms, respectively. The viroid sequences were sequenced which upon BLAST analysis showed 97–98% sequence similarity to other CCHMVd isolates reported from India and world. CCHMVd disease incidence was found to be 15% that was higher in comparison to 8–10% in South Indian states. In phylogenetic analysis, the Palampur isolate grouped with other Indian isolates while isolate YS showed different ancestral origin and grouped separately with other world isolates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Abbreviations

CCHMVd:

Chrysanthemum chlorotic mottle viroid

CSVd:

Chrysanthemum stunt viroid

RT-PCR:

Reverse transcriptase-polymerase chain reaction

References

  • Adkar-Purushothama CA, Chennappa G, Poornachandra Rao K, Sreenivasa MY, Maheshwar MK, Nagendra Prasad MN, Sano T (2017) Molecular diversity among viroids infecting chrysanthemum in India. Vir Genes 53:636–642

    Article  CAS  Google Scholar 

  • De la Pena M, Flores R (2002) Chrysanthemum chlorotic mottle viroid RNA: dissection of the pathogenicity determinant and comparative fitness of symptomatic and non-symptomatic variants. J Mol Bio 321(3):411–421

    Article  Google Scholar 

  • Hosokawa M, Ueda E, Ohishi K, Otake A, Yazawa S (2004) Chrysanthemum stunt viroid disturbs the photoperiodic response for flowering of chrysanthemum plants. Planta 220:64–70

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) In molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Walia Y, Dhir S, Zaidi AA, Hallan V (2015) Apple scar skin viroid naked RNA is actively transmitted by the whitefly Trialeurodes vaporariorum. RNA Biol 12(10):1131–1138

    Article  PubMed  PubMed Central  Google Scholar 

  • Walia Y, Dhir S, Hallan V (2021) Molecular and biological characterisation of an Indian variant of Chrysanthemum stunt viroid. Arch Phytopathol Plant Prot. https://doi.org/10.1080/03235408.2020.1868743

    Article  Google Scholar 

Download references

Acknowledgements

The authors are thankful to the Director, CSIR-IHBT, Palampur for providing necessary research facilities.

Author information

Author notes

  1. Yashika Walia & Sunny Dhir

    Present address: Department of Biotechnology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, HR, 133207, India

Authors and Affiliations

  1. Plant Virology Lab, Council of Scientific & Industrial Research—Institute of Himalayan Bioresource Technology, Palampur, 176061, HP, India

    Yashika Walia, Sunny Dhir & Vipin Hallan

Authors
  1. Yashika Walia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sunny Dhir
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vipin Hallan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Sunny Dhir or Vipin Hallan.

Ethics declarations

Conflict of interests

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file 1

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Walia, Y., Dhir, S. & Hallan, V. Molecular characterization of sequence variants of Chrysanthemum chlorotic mottle viroid from Himachal Pradesh, India. Indian Phytopathology 75, 1241–1244 (2022). https://doi.org/10.1007/s42360-022-00538-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42360-022-00538-0

Keywords

Search

Navigation