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Synthesis, characterization and inhibition effects of Jhingan gum-induced silver nanoparticles against powdery mildew of pea

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Abstract

Jhingan gum, an exudate of Lannea coromandelica (Houtt) tree, induced silver nanoparticles (AgNPs) have been synthesized using green synthetic method and characterized by UV–Vis, Fourier transform infrared spectroscopy (FTIR), Particle Size Analysis, Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). TEM and AFM images of the synthesized AgNPs are monodispersed, almost spherical in shape and falls in nano range (10.39–21.92 nm). The effective hydrodynamic diameter of the particles also corroborated the formation of nanoparticle. The in vitro cytotoxic activity of the synthesized AgNPs against Vero Cell Line, using MTT assay, has shown that the synthesized AgNPs are non-cytotoxic with increased cells viability percentage. In vitro antifungal efficacy of the synthesized AgNPs was found equally effective as protectant and therapeutant against Erysiphe pisi, a plant pathogen. Whereas, in vivo disease inhibitory potential of AgNPs against powdery mildew of pea was found to be 17.43% as compared to commercial formulation (11.19%).

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Acknowledgements

The authors would like to convey their sincere gratitude to Dr. KK Sharma, Director, ICAR-Indian Institute of Natural Resins and Gums, Ranchi (Jharkhand) for constant inspiration and support. The authors are also grateful to Dr. Meena Kataria, Principal Scientist, Division of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly (Uttar Pradesh), India, for conducting cytotoxicity test of the synthesized jhingan gum-induced silver nanoparticles.

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Authors and Affiliations

  1. Processing and Product Development Division, ICAR-Indian Institute of Natural Resins and Gums, Ranchi, 834010, Jharkhand, India

    A. Roy Chowdhury, M. Z. Siddiqui, Ch. Jamkhokai Mate & Md. Ali

  2. ICAR-Indian Institute of Vegetable Research, Varanasi, 221305, India

    Sudarshan Maurya

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  1. A. Roy Chowdhury
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  2. M. Z. Siddiqui
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  4. Ch. Jamkhokai Mate
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Correspondence to A. Roy Chowdhury.

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Chowdhury, A.R., Siddiqui, M.Z., Maurya, S. et al. Synthesis, characterization and inhibition effects of Jhingan gum-induced silver nanoparticles against powdery mildew of pea. Indian Phytopathology 75, 109–116 (2022). https://doi.org/10.1007/s42360-021-00434-z

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