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Development of multiple disease resistant tomato lines through marker assisted breeding and their evaluation for horticultural traits

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Abstract

Development of multiple disease resistant varieties is the most appropriate strategy for the management of losses caused by biotic stresses especially tomato leaf curl and late blight disease. Three genes (Ty-1, Ty-2 and Ty-3) conferring resistance for tomato leaf curl virus and two genes (Ph-2 and Ph-3) conferring resistance against late blight were pyramided from different parents through marker assisted backcrossing with further phenotypic selection. Among 40 BC1F4 advance lines developed through genotypic and phenotypic screening, 13 lines viz. RS-TW-2-4-5G-6, RS-TW-153-1E-1-7, RS-TW-2-4-1-6, RS-TW-2-4-4-8, RS-TW-2-4-6E-9, RS-TW-8-1E-1-13, RS-TW-8-2E-1-13, RS-TW-43-2-1-9, RS-TW-122-4-5-11, RS-TW-128-7-1-11, RS-TW-128-9G-1-13, RS-TW-128-10G-1-11 and RS-TW-130-1E-1-11 were found to possess multiple disease resistance with better horticultural traits as compared to checks. The current study revealed that there was significant improvement in the level of resistance against leaf curl and late blight diseases in tomato cultivars/hybrids with introgression of Ty-1, Ty-2, Ty-3, Ph-2 and Ph-3 genes. The resistant lines developed in the present investigation could be evaluated at multi-locations for their commercial exploitation.

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Acknowledgements

The authors are thankful to Dr. Peter Hanson, Asian Vegetable Research and Development Centre (World Vegetable Centre), Taiwan for providing tomato of leaf curl virus and late blight disease resistant sources. Funding for this investigation granted by the Department of Biotechnology (BT/PR6499/AGII/106/890/2012), Government of India is also acknowledged.

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Authors and Affiliations

  1. Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab, 141004, India

    Ashish Kumar, S. K. Jindal, M. S. Dhaliwal, Abhishek Sharma & Sukhjeet Kaur

  2. Department of Plant Pathology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India

    Sandeep Jain

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  1. Ashish Kumar
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  2. S. K. Jindal
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  3. M. S. Dhaliwal
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  4. Abhishek Sharma
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  5. Sukhjeet Kaur
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  6. Sandeep Jain
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Correspondence to Sukhjeet Kaur.

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Kumar, A., Jindal, S.K., Dhaliwal, M.S. et al. Development of multiple disease resistant tomato lines through marker assisted breeding and their evaluation for horticultural traits. Indian Phytopathology 75, 47–55 (2022). https://doi.org/10.1007/s42360-021-00427-y

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