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Modulated Degradation Rates of Bone Mineral-Like Calcium Phosphate Glass to Support the Proliferation and Osteogenic Differentiation of Bone Marrow-Derived Stem Cells

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Abstract

With an elemental composition similar to bone mineral, and the ability to release phosphorus and calcium that benefit bone regeneration, Calcium Phosphate Glass (CPG) serves as a promising component of bone tissue engineering scaffolds. However, the degradation of CPG composites typically results in increased acidity, and its impact on bone-forming activity is less studied. In this work, we prepared 3D-printed composite scaffolds comprising CPG, Poly-ε-caprolactone (PCL), and various Magnesium Oxide (MgO) contents. Increasing the MgO content effectively suppressed the degradation of CPG, maintaining a physiological pH of the degradation media. While the degradation of CPG/PCL scaffolds resulted in upregulated apoptosis of Rat Bone Marrow-derived Stem Cells (rBMSC), scaffolds containing MgO were free from these negative impacts, and an optimal MgO content of 1 wt% led to the most pronounced osteogenic differentiation of rBMSCs. This work demonstrated that the rapid degradation of CPG impaired the renewability of stem cells through the increased acidity of the surrounding media, and MgO effectively modulated the degradation rate of CPG, thus preventing the negative effects of rapid degradation and supporting the proliferation and osteogenic differentiation of the stem cells.

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Data availability

The authors confirm that the data supporting the findings of this study are available within the article. Raw data that support the findings of this study are available from the corresponding author (X.G.) upon reasonable request.

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Acknowledgements

This work received financial support from the National Key Research and Development Program of China (Grant No. 2018YFA0703000), the National Natural Science Foundation of China (Grant Nos. 52250006, 52075482), the Ningbo Top Medical and Health Research Program (Grant No. 2022020304), and the Ningbo Key Science and Technology Major Project (Grant No. 2022Z143).

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  1. Lizhe He and Yuye Huang have contributed equally to this work.

Authors and Affiliations

  1. Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, 315010, China

    Lizhe He & Xiang Gao

  2. Center for Medical and Engineering Innovation, The First Affiliated Hospital of Ningbo University, Ningbo, 315010, China

    Lizhe He & Yuye Huang

  3. The State Key Laboratory of Fluid Power Transmission and Control Systems, Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou, 310028, China

    Lizhe He & Jun Yin

  4. Faculty of Science and Engineering, University of Nottingham Ningbo China, Ningbo, 315100, China

    Jiafei Gu & Xiaoling Liu

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He, L., Huang, Y., Gu, J. et al. Modulated Degradation Rates of Bone Mineral-Like Calcium Phosphate Glass to Support the Proliferation and Osteogenic Differentiation of Bone Marrow-Derived Stem Cells. J Bionic Eng (2024). https://doi.org/10.1007/s42235-024-00540-4

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