Abstract
In a greenhouse complex located in the northwest of Iran, yellow-colored spots and deformation were observed on the fruit of bell pepper plants. Analysis of ten collected fruits showed the presence of both tomato brown rugose fruit virus (ToBRFV) and tomato spotted wilt virus (TSWV) viruses, indicating mixed-infection in three of the ten sampled pepper fruits. The S segment of TSWV was amplified using specific primers for the nonstructural protein (NSs), intergenic regions (IGR), and nucleocapsid protein (NP) gene. Sequence comparison of the coat protein (CP) gene of the ToBRFV isolate revealed that the new ToBRFV isolate formed a sister group with the previously reported Iran-1 isolate from Iran. Phylogenetic analysis of the S segment and its two ORFs (NSs and N) resulted in three groups. The analysis based on NSs and N protein sequences in RNA S showed that the Iranian TSWV isolate clustered in cluster I and formed a sister group with isolates from France and Iran. On the other hand, the analysis of RNA S segments revealed that the Iranian TSWV isolate clustered together with isolates from the USA, Turkey, and Italy. Transmission ability of the mixed virus sample was confirmed by mechanical inoculation of pepper and tomato plants. The plants showed a range of symptoms 15–25 days post inoculation (dpi).
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Data availability
The sequences data were deposited in NCBI-Genbank.
References
Amer MA, and Mahmoud SY (2020) First report of Tomato brown rugose fruit virus on tomato in Egypt. New Dis Rep. https://doi.org/10.5197/j.2044-0588.2020.041.024
Cambrón-Crisantos JM, Rodríguez-Mendoza J, Valencia-Luna JB, Rangel SA, De Jesús G-Ávila C, López-Buenfil JA (2018) First report of tomato brown rugose fruit virus (ToBRFV) in Michoacan, Mexico. M. J. Plant Pathol 37(1):185–192
Esmaeilzadeh F, Koolivand D (2021) Occurrence of tomato brown rugose fruit virus in tomato in Iran. J Plant Pathol. https://doi.org/10.1007/s42161-021-01009-7
Esmaeilzadeh F, Koolivand D (2022) First report of tomato brown rugose fruit virus infecting bell pepper in Iran. J Plant Pathol. https://doi.org/10.1007/s42161-022-01094-2
Gambino G, Perrone I, Gribaudo I (2008) A Rapid and Effective Method for RNA Extraction from Different Tissues of Grapevine and Other Woody Plants. Phytochem Anal 19:520–525
Ghorbani A, Rostami M, Seifi S, Izadpanah K (2021) First report of Tomato brown rugose fruit virus in greenhouse tomato in Iran. New Dis Rep 44. https://doi.org/10.1002/ndr2.12040
Hall TA (1999) BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Klap C, Luria N, Smith E, Hadad L, Bakelman E, Sela N, Belausov E, Lachman O, Leibman D, Dombrovsky A (2020) Tomato Brown Rugose Fruit Virus Contributes to Enhanced Pepino Mosaic Virus Titers in Tomato Plants. Viruses 12:879. https://doi.org/10.3390/v12080879
Lukman R, Afifuddin A, Deynze AV, Hill T, Jimenez R (2019) A survey of mixed Begomovirus infection in solanaceae and fabaceae at different altitudes in East Java. Indonesia Arch Phytopathol 52(3–4):385–406. https://doi.org/10.1080/03235408.2019.1625590
Luria N, Smith E, Reingold V, Bekelman I, Lapidot M, Levin I, Elad N (2017) A new Israeli Tobamovirus isolate infects tomato plants harboring Tm-22 resistance genes. PLoS ONE 12:1–19
Martin DP, Murrell B, Khoosal A, Muhire B (2017) Detecting and Analyzing Genetic Recombination Using RDP4. Methods Mol Biol 525:433–460
Salem N, Mansour A, Ciuffo M, Falk BW, Turina M (2016) A new tobamovirus infecting tomato crops in Jordan. Arch Virol 161:503–506
Scholthof KBG, Adkins S, Czosnek H, Palukaitis P, Jacquot E, Hohn T, Hohn B, Saunders K, Candresse T, Ahlquist P et al (2011) Top 10 plant viruses in molecular plant pathology. Mol Plant Pathol 12:938–954
Singhal P, Nabi SU, Yadav MK, Dubey A (2020) Mixed infection of plant viruses: diagnostics, interactions and impact on host. J Plant Dis Prot. https://doi.org/10.1007/s41348-020-00384-0
Tamura K, Stecher G, Kumar S (2021) Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol 38(7):3022–3027. https://doi.org/10.1093/molbev/msab120
Zhang S, Griffiths JS, Marchand G, Bernards MA, Wang A (2022) Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide. Mol Plant Pathol 23:1262–1277. https://doi.org/10.1111/mpp.13229
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D.K. conceived and designed the study. F.E. conducted the experiments and collected the data. D.K. and F.E. analyzed the data and wrote the manuscript. Authors contributed to interpreting the results, reviewing and editing the manuscript, and approving the final version.
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Esmaeilzadeh, F., Koolivand, D. Co-infection of tomato brown rugose fruit virus and tomato spotted wilt virus in pepper from Iran. J Plant Pathol 105, 1129–1134 (2023). https://doi.org/10.1007/s42161-023-01436-8
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DOI: https://doi.org/10.1007/s42161-023-01436-8