Teaplant (Camellia sinensis (L.) O. Kuntze) is an importantly economical crop in China. In July of 2018, chlorosis and downward leafroll, some of typical symptoms of disease caused by grapevine leafroll-associated viruses (GLRaVs), members of species in the family Closteroviridae (Al Rwahnih et al. 2012; Reynard et al. 2015), were observed on leaves of teaplant with green vein in Daolang Town, Tai’an City. In June of 2019, a field survey was carried out to assess the sanitary status of teaplants in the Daolang region of China. This teayard was near grapevine yards heavily infected with GLRaV-7. Based on the prevalence of GLRaV-7 near this teayard and the occurrence of delphacidae, the infection of GLRaVs, especially GLRaV-7, had been suspected in this teayard. The leaf samples of teaplant were taken from symptomatic plants and asymptomatic nearby ones. A total of 436 samples was collected and tested for the detection of five GLRaVs using five commercial ELISA kits (GLRaV-1 DAS, GLRaV-2 DAS, GLRaV-3 DAS, GLRaV-4 DAS and GLRaV-7 DAS, Invitrogen™, U.S.A.). The results showed that twenty-one samples (five symptomatic ones and sixteen asymptomatic ones) were positive for the detection of GLRaV-7. Total RNA was extracted from twenty-one positive samples by a Trizol Kit (Invitrogen™, U.S.A.) and reverse transcription (RT)-PCRs were performed using the specific primer pair: GLRV-7-P61F (5′-GGTTTGAAATGGAAAACATGATAC-3′) and GLRV-7-P61R (5′-CACGTTTAGTTGAATTGGTTAATC-3′) (Lyu et al. 2014) specific to the p61 gene of GLRaV-7. The twenty-one positive samples respectively produced one single band of 518 bp. The PCR products were purified using a DNA Purification Kit (Solarbio™, China), sequenced and analyzed by BLAST. The twenty-one isolates selected for sequence analysis had identical gene sequences, and hence, only one sequence for isolate TPSD1 was submitted to GenBank (accession number MW258966) which showed 97 to 99% nucleotide identity and 100% amino acid identity with various GLRaV-7 isolates in the NCBI database. Negative ELISA samples had been randomly tested by RT-PCR, but no amplicon was obtained in these samples using the same primer pair. To our knowledge, this is the first occurrence of GLRaV-7 in teaplants in China. This finding is important because this virus affects yield and quality of teaplants.
References
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Xie, X., Zhu, C. & Han, X. First report on the occurrence of grapevine leafroll-associated virus 7 in teaplants. J Plant Pathol 104, 455 (2022). https://doi.org/10.1007/s42161-021-01011-z
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DOI: https://doi.org/10.1007/s42161-021-01011-z