Avoid common mistakes on your manuscript.
Euphorbia leaf curl virus (EuLCV) is a member of the genus Begomovirus in the family Geminiviridae, which has a circular single-stranded DNA genome and considered as a monopartite Begomovirus without betasatellite. It was firstly found infecting Euphorbia pulcherrima in Guangxi province, China (Ma et al. 2004), and was reported on Passion fruit (Passiflora edulis) in Taiwan island and South Korea (Cheng et al. 2014; Ko et al. 2016). In 2016–2019, Passiflora edulis with stunted, chlorotic and malformation symptoms were found in Xiamen City, Fujian province, China.
ELISA was conducted with the antibody specific for Begomovirus, 6 samples showed positive reaction.
To confirm the infection of EuLCV, total DNA were extracted from 6 symptomatic leaf samples with a Hi-DNAsecure Plant Kit (TIANGEN BIOTECH(BEIJING) CO., LTD). PCR with the specific primers 1F (5′-CGTGCTTATGTCCCTACTGTCC-3′) and 2R (5′-ACCGTCCAAGTACAATTCAATCACA-3′) were conducted to amplify the genome of DNA molecules. An expected size (946 bp) PCR amplicon was obtained in 1 out of 6 samples, then was cloned and sequenced (GenBank Accession No. MT360641). BLASTn analysis revealed that this fragment shared high nucleotide sequence identity (91–99.15%) with other EuLCV isolates. The isolate shared 99.15% nucleotide identity with EuLCV isolate PF1 (KC161185.1) from Taiwan island, and 99.05% nucleotide identity with EuLCV isolate JJ17-PF7 and JJ17-PF10 (MK066926.1 and MK066927.1). To our knowledge, this is the first report of EuLCV infecting Passiflora edulis in Fujian province, China. Further investigation on the genome and the prevalence of this virus is required.
Data availability statement
All data included in this study are available upon request by contact with the corresponding author.
References
Cheng YH et al (2014) First report of Euphorbia leaf curl virus and Papaya leaf curl Guangdong virus on passion fruit in Taiwan. Plant Dis 98(12):1746–1746
Ko, Lee S-J et al (2016) First report of Euphorbia leaf curl virus in passion fruits in South Korea and its natural occurrence in papaya. Plant Dis 100(4):865
Ma XY et al (2004) Molecular characterization of a distinct Begomovirus infecting Euphorbia pulcherrima in China. J Phytopathol 152(4):215–218
Acknowledgements
This work is supported by the Science and Technology Program of Xiamen City, China (3502Z20204503-2) and (3502Z20162013).
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Ye, T., Zheng, GH., Guo, Y. et al. First report of Euphorbia leaf curl virus infecting Passiflora edulis in Fujian province, China. J Plant Pathol 104, 807 (2022). https://doi.org/10.1007/s42161-021-00941-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42161-021-00941-y