Abstract
Spinach (Spinacia oleracea L.), is a commercially important, widely grown and consumed vegetable in Turkey. In October 2020, typical symptoms resembling powdery mildew were observed on spinach plants in two inspected fields in Hatay province of Turkey. Circular discolorations patches accompanied with dull whitish fluffy sporulation were observed on both upper and lower leaf surfaces of plants. Conidiophores are typically formed from internal mycelium emerging through stomatal openings, either singly or in groups. Morphologically distinguished dimorphic conidia without distinct fibrosin bodies, are formed singly at the apex of conidiophores. No chasmothecia were found on heavily infected leaves over the course of the season. The identity of the disease agent was further confirmed sequencing of the ITS rDNA region of representative isolates by using ITS5/P3 and ITS1/ITS4 primers. Pathogenicity was confirmed through inoculation by touching freshly sporulating plant leaves onto the leaves of potted healthy spinach plants. Based on morphological characteristics of asexual fungal structures, molecular sequencing and pathogenicity test, the causal disease agent responsible for the spinach powdery mildew was identified as Leveillula taurica (Lév.) G. Arnaud (syn. = Oidiopsis haplophylli). To our knowledge, this is the first report of powdery mildew caused by L. taurica on S. oleracea in Turkey.
References
Anonymous (2018) Food and agriculture organization of the United Nations. Statistical database. http://www.fao.org/faostat/en/#data/QC/visualize. Accessed 08 November 2020
Anonymous (2020) TUÄ°K Bitkisel Ãœretim Ä°statistikleri. Internet Resource: https://data.tuik.gov.tr/Bulten/Index?p=Crop-Production-2nd-Estimation-2020-33736. Accessed 08 November 2020
Amano K (1986) Host range and geographical distribution of the powdery mildew fungi. Scientific Societies Press, Tokyo, Japan
Braun U (1987) A monograph of the Erysiphales (Powdery Mildews). Beihefte zur Nova Hedwigia 89:1–700
Braun U, Cook RTA, Inman AJ, Shin HD (2002) The taxonomy of the powdery mildew fungi. In: Belánger RR, Bushnell WR, Dick AJ, Carver TLW (eds) The Powdery Mildews: A Comprehensive Treatise. American Phytopathological Society Press, St. Paul, MN, USA, pp 13–55
Braun U, Cook RTA (2012) Taxonomic Manual of the Erysiphales (Powdery Mildews). CBS Biodiversity Series No. 11: 703 pp, CBS, Utrecht, the Netherlands
Correll JC, Bluhm BH, Feng C, Lamour K, du Toit LJ, Koike ST (2011) Spinach: Better management of downy mildew and white rust through genomics. Eur J Plant Pathol 129:193–205. https://doi.org/10.1007/s10658-010-9713-y
Farr DF, Rossman AY (2020) Fungal Databases, Systematic Botany & Mycology Laboratory, ARS, USDA. http://nt.ars-grin.gov/fungaldatabases/. Accessed 04 November 2020
Koike ST, Feng C, Correll JC (2015) Powdery mildew, caused by Leveillula taurica, on spinach in California. Plant Dis 99:555. https://doi.org/10.1094/PDIS-09-14-0977-PDN
Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054
Kusch S, Németh MZ, Vaghefi N, Ibrahim HM, Panstruga R, Kiss L (2020) A short-read genome assembly resource for Leveillula taurica causing powdery mildew disease of sweet pepper (Capsicum annuum). Mol Plant-Microbe Interact 33:782–786. https://doi.org/10.1094/MPMI-02-20-0029-A
Nakova M (2012) Powdery mildew on spinach (Spinacia oleracea L.). Pestic Phytomed (Belgrade) 27:151–155. https://doi.org/10.2298/PIF1202151N
Park MJ, Han JG, Shin HD (2008) A novel finding of powdery mildew caused by Oidiopsis haplophylli on spinach. Plant Pathol 57:1175–1175. https://doi.org/10.1111/j.1365-3059.2008.01913.x
Soylu EM, Soylu S (2003) Light and electron microscopy of the compatible interaction between Arabidopsis and the downy mildew pathogen Peronospora parasitica. J Phytopathol 151:300–306. https://doi.org/10.1046/j.1439-0434.2003.00723.x
Soylu S, Kara M, Kurt S, Uysal A, Shin HD, Choi YJ, Soylu EM (2018a) First report of white blister rust disease caused by Albugo occidentalis on spinach in Turkey. Plant Dis 102:826–827. https://doi.org/10.1094/PDIS-10-17-1561-PDN
Soylu EM, Kara M, Kurt S, Uysal A, Shin HD, Choi YJ, Soylu S (2018b) First report of downy mildew disease caused by Peronospora effusa on spinach in Turkey. Plant Dis 102:1854–1854. https://doi.org/10.1094/PDIS-01-18-0102-PDN
Takamatsu S, Heluta V, Havrylenko M, Divarangkoon R (2009) Four powdery mildew species with catenate conidia infect Galium: Molecular and morphological evidence. Mycol Res 113:117–129. https://doi.org/10.1016/j.mycres.2008.09.006
Uysal A, Kurt S (2017) Influence of inoculum density, temperature, wetness duration, and leaf age on infection and development of spinach anthracnose caused by the fungal pathogen Colletotrichum spinaciae. Eur J Plant Pathol 149:1041–1052. https://doi.org/10.1007/s10658-017-1249-y
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Soylu, S., Uysal, A., Kurt, Ş. et al. Morphological and molecular characterization of spinach powdery mildew disease caused by Leveillula taurica in Turkey. J Plant Pathol 103, 955–959 (2021). https://doi.org/10.1007/s42161-021-00828-y
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DOI: https://doi.org/10.1007/s42161-021-00828-y