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Influence of inoculum density, temperature, wetness duration, and leaf age on infection and development of spinach anthracnose caused by the fungal pathogen Colletotrichum spinaciae

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Abstract

Spinach anthracnose caused by Colletotrichum spinaciae is a newly emergent disease that causes serious losses in the spinach fields of the Mediterranean Region of Turkey. The most popular spinach cv. Matador is very susceptible to the anthracnose. Currently, anthracnose disease is not effectively controlled in Turkey due to the lack of understanding of its epidemiology. In order to understand the effect of environmental parameters on anthracnose, the influences of inoculum concentration, temperature, leaf wetness duration, plant and leaf age on lesion development of spinach anthracnose was studied under controlled conditions. Seedlings of spinach cv. Matador at 4-leaf stage were inoculated with four inoculum concentrations, 102, 104, 106, 2 × 106 conidia/ml, and subjected to seven temperatures of 15, 18, 22, 25, 28, 30, and 33 °C, and nine leaf wetness durations of 3, 5, 10, 20, 24, 30, 44, 48, and 53 h. The mean number of lesions per plant increased at the rate of 50.7% with increasing inoculum density from 104 to 106 conidia per millilitre. Inoculum concentration with 106 conidia per ml of C. spinaciae was appropriate for infection and lesion development. Disease was observed at all temperatures and increased with wetness duration. The development of lesions per plant was observed more gradually when incubation temperature increased from 15 °C to 22 °C. The mean lesion number of anthracnose increased on spinach seedlings as wetness duration increased to 24 h and temperatures to 22 °C and 25 °C. Regression analysis revealed that there was a linear relationship between the increase in number of lesions per plant and wetness duration at 18, 25, and 30 °C. Optimum disease development was recorded at 22 °C with 24 h leaf wetness period. The highest number of lesions was observed at 45 d-old plants. In the same time, older leaves were more severely infected than younger leaves. This kind of information can be used to target key stages of an epidemic and may allow tailoring of spray programs or disease forecasts to cultivar with different levels of anthracnose resistance.

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References

  • Byrne, J. M., Hausbeck, M. K., Meloche, C., & Jarosz, A. M. (1998). Influence of dew period and temperature on foliar infection of greenhouse-grown tomato by Colletotrichum coccodes. Plant Disease, 82, 639–641.

    Article  Google Scholar 

  • Campbell, C. L., & Madden, L. V. (1990). Introduction to plant disease epidemiology. New York: John Wiley & Sons.

    Google Scholar 

  • Cannon, P. F., Damm, U., Johnston, P. R., & Weir, B. S. (2012). Colletotrichum-current status and future directions. Studies in Mycology, 73, 181–213.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cerkauskas, R. F., Nauta, R., & McDonald, M. R. (1991). First report of spinach anthracnose in Ontario. Plant Disease, 75, 101.

    Article  Google Scholar 

  • Chongo, G., & Bernier, C. C. (2000). Effects of host, inoculum concentration, wetness duration, growth stage, and temperature on anthracnose of lentil. Plant Disease, 84, 544–548.

    Article  Google Scholar 

  • Correll, J. C., Morelock, T. E., Black, M. C., Koike, S. T., Brandenberger, L. P., & Dainello, F. J. (1994). Economically important diseases of spinach. Plant Disease, 78, 653–660.

    Article  Google Scholar 

  • Damicone, J., Pierson, T., & Stoner, A. (2013). Biology and management of spinach anthracnose. Phytopathology, 103(Suppl.1), S1.3.

    Google Scholar 

  • Damm, U., Woudenberg, J. H. C., Cannon, P. F., & Crous, P. W. (2009). Colletotrichum species with curved conidia from herbaceous hosts. Fungal Diversity, 39, 45–87.

    Google Scholar 

  • Diéguez-Uribeondo, J., Förster, H., & Adaskaveg, J. E. (2011). Effect of wetness duration and temperature on the development of anthracnose on selected almond tissues and comparison of cultivar susceptibility. Phytopathology, 101, 1013–1020.

    Article  PubMed  Google Scholar 

  • Dillard, H. R. (1989). Effects of temperature, wetness duration, and inoculum density on infection and lesion development of Colletotrichum coccodes on tomato fruit. Phytopathology, 79, 1063–1066.

    Article  Google Scholar 

  • Ellis, J. B., & Halsted, B. D. (1890). New fungi. Journal of Mycology, 6, 34–35.

    Google Scholar 

  • FAOSTAT (2015). FAOSTAT online database. http://www.faostat3.fao.org/browse/Q/QC/E.

  • Guyot, J., Omanda, E. N., & Pinard, F. (2005). Some epidemiological investigations on Colletotrichum leaf disease on rubber tree. Crop Protection, 24, 65–77.

    Article  Google Scholar 

  • Harp, T., Kuhn, P., Roberts, P. D., & Pernezny, K. L. (2014). Management and cross-infectivity potential of Colletotrichum acutatum causing anthracnose on bell pepper in Florida. Phytoparasitica, 42, 31–39.

    Article  CAS  Google Scholar 

  • Hong, J. K., & Hwang, B. K. (1998). Influence of inoculum density, wetness duration, plant age, inoculation method, and cultivar resistance on infection of pepper plants by Colletotrichum coccodes. Plant Disease, 82, 1079–1083.

    Article  Google Scholar 

  • Kim, Y. J., Hwang, B. K., & Park, K. W. (1989). Expression of age-related resistance in pepper plants infected with Phytophthora capsici. Plant Disease, 73, 745–747.

    Article  Google Scholar 

  • Koike, S. T., & Correll, J. C. (1993). First report of spinach anthracnose caused by Colletotrichum dematium in California. Plant Disease, 77, 318.

    Article  Google Scholar 

  • Kurt, Ş., & Tok, F. M. (2006). Influence of inoculum concentration, leaf age, temperature, and duration of leaf wetness on Septoria blight of parsley. Crop Protection, 25, 56–561.

    Article  Google Scholar 

  • Kurt, Ş., Uysal, A., & Soylu, E. M. (2014). Reactions of differential host plant species and some cultivars against anthracnose disease (Colletotrichum spinaciae) in spinach. 7th symposium of National Vegetable Agriculture, 2-4 September, Tekirdağ, Turkey, p.134.

  • Kurt, Ş., Uysal, A., & Akgül, D. S. (2016). First report of anthracnose caused by Colletotrichum spinaciae on spinach in the Mediterranean region of Turkey. Plant Disease, 100(1), 219.

    Article  Google Scholar 

  • Larsson, M., & Gerhardson, B. (1992). Disease progression and yield losses from root diseases caused by soilborne pathogens of spinach. Phytopathology, 82, 403–406.

    Article  Google Scholar 

  • Leandro, L. F. S., Gleason, M. L., Nutter Jr., F. W., Wegulo, S. N., & Dixon, P. M. (2003). Influence of temperature and wetness duration on conidia and appressoria of Colletotrichum acutatum on symptomless strawberry leaves. Phytopathology, 93, 513–520.

    Article  CAS  PubMed  Google Scholar 

  • Lima, N. B., Lima, W. G., Towar-Pedreza, J. M., Michereff, S. J., & Camara, M. P. S. (2015). Comparative epidemiology of Colletotrichum species from mango in northeastern Brazil. European Journal of Plant Pathology, 141, 679–688.

    Article  Google Scholar 

  • Monroe, J. S., Santini, J. B., & Latin, R. (1997). A model defining the relationship between temperature and leaf wetness duration, and infection of watermelon by Colletotrichum orbiculare. Plant Disease, 81, 739–742.

    Article  Google Scholar 

  • Moral, J., Jurado-Bello, J., Sánchez, M. I., Oliveira, R., & Trapero, A. (2012). Effect of temperature, wetness duration, and planting density on olive anthracnose caused by Colletotrichum spp. Phytopathology, 102, 974–981.

    Article  PubMed  Google Scholar 

  • Rodriguez-Salamanca, L. M., Quesada-Ocampo, L. M., Naegele, R. P., & Hausbeck, M. K. (2015). Characterization, virulence, epidemiology, and management of leaf curling and petiole anthracnose in celery. Plant Disease, 99, 1832–1840.

    Article  Google Scholar 

  • Sherf, A. F., & MacNab, A. A. (1986). Vegetable diseases and their control (Second ed.p. 728). New York: John Wiley and Sons.

    Google Scholar 

  • Sosnowski, M. R., Scott, E. S., & Ramsey, M. D. (2005). Temperature, wetness period and inoculum concentration influence infection of canola (Brassica napus) by conidia of Leptosphaeria maculans. Australasian Plant Pathology, 34, 339–344.

    Article  Google Scholar 

  • Sullivan, M. J., Damicone, J. P., & Payton, M. E. (2002). The effects of temperature and leaf wetness period on the development of spinach white rust. Plant Disease, 86, 753–758.

    Article  Google Scholar 

  • Thompson, D. C., & Jenkins, S. F. (1985). Effect of temperature, moisture and cucumber resistance on lesion size and increase and conidial production by Colletotrichum lagenarium. Phytopathology, 75, 828–832.

    Article  Google Scholar 

  • TUIK (2015). Crop production statistics. Turkish Statistical Institute, Ankara. http://www.turkstat.gov.tr/.

  • Von Arx, J. A. (1970). A revision of the fungi classified as Gloeosporium. Bibliography Mycology, 24, 1–203.

    Google Scholar 

  • Washington, W. S., Irvine, G., Aldaoud, R., De Alwis, S., Edwards, J., & Pascoe, I. G. (2006). First record of anthracnose of spinach caused by Colletotrichum dematium in Australia. Australasian Plant Pathology, 35, 89–91.

    Article  Google Scholar 

  • Wilson, L. L., Madden, L. V., & Ellis, M. A. (1990). Influence of temperature and wetness duration on infection of immature and mature strawberry fruit by Colletotrichum acutatum. Phytopathology, 80, 111–116.

    Article  Google Scholar 

Download references

Acknowledgements

Authors would like to thank Professor Emine Mine Soylu for her scientific and technical support. This investigation was supported by a Grant (No. 10720) from the Coordinatorship of the Scientific Research Projects of Mustafa Kemal University, Hatay, Turkey.

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  1. Department of Plant Protection, Faculty of Agriculture, and Centre for Implementation and Research of Plant Health Clinic, Mustafa Kemal University, 31040, Antakya, Hatay, Turkey

    Aysun Uysal & Şener Kurt

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  1. Aysun Uysal
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  2. Şener Kurt
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Correspondence to Şener Kurt.

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Uysal, A., Kurt, Ş. Influence of inoculum density, temperature, wetness duration, and leaf age on infection and development of spinach anthracnose caused by the fungal pathogen Colletotrichum spinaciae . Eur J Plant Pathol 149, 1041–1052 (2017). https://doi.org/10.1007/s10658-017-1249-y

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