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Nigrospora sphaerica (Sacc.) E.W. Mason is a seriously damaging pathogen causing leaf blight on a few plant species. In July 2018, a leaf blight of unknown etiology was observed on peanut (Arachis hypogaea Linn.) leaves in Wenchang, China. Initial symptoms included circular brown spots, 2 mm in diameter, surrounded by yellow haloes, gradually developing into circular or elliptical necroses of up to 6 mm in diameter. Symptomatic tissues were surface-sterilized with 70% ethanol for 30 s and 0.1% HgCl2 for 60 s, washed three times in sterile water and put on PDA at 25 °C. After 5 days at 25 ± 2 °C, the fungus developed flat, white and light-to-dark grey colonies, with white-to-dark brown septate branched hyphae. Conidia typical for Nigrospora were black, one-celled (10.2–14.6 μm), and borne singly at the tips of conidiophores. The internal transcribed spacer (ITS) region of the rDNA was sequenced using ITS1/ITS4 primers and accession No. MK482388 was 99% identical to N. sphaerica accession Nos. MG669225.1 and HQ608063.1. For pathogenicity testing, a conidial suspension (106 conidia ml−1) from a 7-day-old culture of N. sphaerica was used to inoculate ten leaves of yue-you 7 peanut cultivar, followed by incubation in a controlled environment chamber at 25 °C with 70–80% humidity. As a control, ten leaves of yue-you 7 peanut cultivar were inoculated with sterile distilled water. Two weeks after inoculation, symptoms were observed only on the leaves inoculated with conidia and the fungus was consistently re-isolated. Nigrospora sphaerica was observed on blueberry in Argentina, sugarcane in China, and tea in India (Cui et al. 2018; Dutta et al. 2015). To our knowledge, this is the first report of N. sphaerica on peanut.
References
Cui YP, Peng AT, Wu B, Lin JF, Song XB, Li ZL (2018) First report of Nigrospora leaf blight on sugarcane caused by Nigrospora sphaerica in China. Plant Dis 102(4):824. https://doi.org/10.1094/PDIS-07-17-1060-PDN
Dutta J, Gupta S, Thakur D, Handique PJ (2015) First report of Nigrospora leaf blight on tea caused by Nigrospora sphaerica in India. Plant Dis 99(3):417. https://doi.org/10.1094/PDIS-02-14-0196-PDN
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This study was supported by the Key Research and Development Project in Hainan Province (ZDYF2018100).
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Liu, X., Yu, F., Fu, D. et al. First report of leaf blight on peanut caused by Nigrospora sphaerica in China. J Plant Pathol 102, 1269 (2020). https://doi.org/10.1007/s42161-020-00552-z
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DOI: https://doi.org/10.1007/s42161-020-00552-z