In June 2017, a leaf spot disease on Kudzu (Pueraria lobata) was observed in Guixi city of Jiangxi province, China, with a disease incidence of approximately 30%. The symptoms initially appeared as dark brown lesions surrounded by yellow halos, and later became larger (5–12 mm in diameter), circular or angular spots with gray centers and dark brown margins. Conidiophores arising from stromata were densely fasciculate, pale olivaceous to pale brown, erect, straight or slightly curved, unbranched, 18.0 to 73.0 × 2.7 to 4.3 μm, and 0 to 3 septate. Conidiogenous loci were inconspicuous, neither thickened nor darkened. Conidia were obclavate, pale olivaceous, straight to slightly curved, with rounded apex and base truncated, 36.4 to 137.8 × 3.4 to 5.0 μm, and 3 to 12 septate. Based on morphology, the pathogen was identified as Pseudocercospora puerariicola Deighton (Guo and Hsieh 1995). Isolation was performed from the infected leaves to PDA using single-spore isolation method. After 26-day incubation at 28 °C, 2.8 to 3.1 cm grayish brown colonies were observed. The ITS region was amplified using ITS1/ITS4 primers (White et al. 1990) and deposited in GenBank (MG601510). A BLAST search revealed 99% identity with that of P. puerariicola isolate TNM3769 from Taiwan (KJ201939) (Kirschner and Liu 2014). A voucher specimen (JXAU-H2017188) was deposited in the Herbarium of College of Agronomy, Jiangxi Agricultural University. A conidial suspension (1 × 105 conidia/ml) was sprayed on healthy plants incubated in a greenhouse at 28 °C, while control plants were sprayed with sterilized water. After 2 to 3 weeks, typical symptoms similar to those in the field appeared on the inoculated leaves, whereas the control leaves remained symptomless. P. puerariicola was successfully re-isolated from these diseased leaves. P. puerariicola was previously observed from some provinces in China, including Yunnan, Anhui, Zhejiang, Taiwan, Hunan, Guangdong, Guangxi (Tai 1979; Guo and Hsieh 1995). According to our knowledge, this is the first report of P. puerariicola causing leaf spot on kudzu in Jiangxi China.