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Nanobody immobilization on magnetic nanoparticles via monomeric streptavidin-biotin specific interaction for aflatoxin adsorption

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Abstract

A new magnetic aflatoxin (AF) adsorbent was synthesized by nanobody (Nb28) immobilization on magnetic nanoparticles (MNPs). Monomeric streptavidin (mSA)-biotin specific interaction technology was used as a linker for nanobody immobilization. As a magnetic solid support, Fe3O4 nanoparticles were modified with tetraethyl orthosilicate (TEOS), (3-Aminopropyl) trimethoxysilane (APTMS), and glutaraldehyde (GA). According to the characterization results, modified MNPs were monodisperse, paramagnetic, with average diameter of 450 nm and they showed less susceptibility to oxidation on air. MNPs-Nb28 and MNPs-mSA-Nb28 enzymatic activity was analyzed by high-performance liquid chromatography (HPLC) and showed the highest level of adsorption under optimal conditions for aflatoxin G2 (AFG2), reaching concentration reduction up to 96.9% and 97.1%, respectively. MNPs-Nb28 showed higher stability over storage time, temperature, and pH compare to MNPs-mSA-Nb28. The maximum AF adsorption capacities of MNPs-Nb28 and MNPs-mSA-Nb28 were 193 µg·g−1 and 194 µg·g−1, respectively. Both nanocomposites were proven to be a reliable tool for fast and efficient removal of different aflatoxins (AFs) from solution.

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Funding

This work was supported by “Pioneer” and “Leading Goose” R&D Program of Zhejiang (2022C02023); Basic Research Special Fund Project of Zhejiang University of Science and Technology (2023QN024; 2023JLZD007); China Agricultural Research System of MOF and MARA (CARS-21). The authors are also thankful to Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing for providing necessary equipment support.

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  1. Hongpeng Wang, Alexey Tarabarov, and Qingqing Rao contributed equally to the work and can be treated as co-first authors.

Authors and Affiliations

  1. Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, People’s Republic of China

    Hongpeng Wang, Alexey Tarabarov, Yongqi Wang, Zhuqian Xiao, Changjiang Lv, Jiayao Yang & Jun Huang

  2. College of Chemical and Materials Engineering, Zhejiang A&F University, Hangzhou, 311300, People’s Republic of China

    Qingqing Rao, Xing Wang, Yiyu Qi & Shengxiang Yang

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Contributions

Hongpeng Wang: review and editing and supervision; Alexey Tarabarov: visualization, investigation, writing original draft, and formal analysis; Qingqing Rao: review and editing and discussion; Xing Wang: project administration, resources; Yiyu Qi: data acquisition; Yongqi Wang: data curation; Zhuqian Xiao: methodology; Changjiang Lv: computation; Jiayao Yang: validation; Jun Huang: funding acquisition; Shengxiang Yang: conceptualization. All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.

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Wang, H., Tarabarov, A., Rao, Q. et al. Nanobody immobilization on magnetic nanoparticles via monomeric streptavidin-biotin specific interaction for aflatoxin adsorption. Adv Compos Hybrid Mater 7, 94 (2024). https://doi.org/10.1007/s42114-024-00893-8

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