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Development of Aflatoxin B1 Aptamer Sensor Based on Iron Porphyrin Organic Porous Material

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Abstract

In this paper, using molybdenum disulfide doped with gold nanoclusters (Au NCs@MoS2) as the substrate, an aflatoxin aptamer sensor was constructed utilizing iron porphyrin organic porous material modified with streptavidin and loaded with gold nano-bipyramids (SA-Au NBPs@ porous aromatic framework (PAF)-40-Fe) as a label. The catalyzed reduction current of hydrogen peroxide (H2O2) by Au NBPs@PAF-40-Fe was detected which is proportional to the concentration of aflatoxin B1. Thereby, quantitative detection of aflatoxin B1 (AFB1) is achieved. Under the optimal conditions, the linear range of the sensor is 0.05 ~ 85 ng/mL with detection limit of 0.017 ng/mL and the linear correlation coefficient (R2) of 0.9825. The average recovery is 99.28 %. This aflatoxin aptamer sensor has good selectivity and provides a highly sensitive method for the detection of aflatoxin.

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Funding

This work was supported by the National Natural Science Foundation of China (grant 21765026 and 21465026).

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Author notes

  1. Min Wang and Mengting Duan contributed equally

Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming, Yunnan, 650500, People’s Republic of China

    Min Wang, Mengting Duan, Fengxian Yu, Xuewen Fu, MengQiao Gu, KuanNeng Chi, Mei Li, Xiaojuan Xia, Rong Hu, Yunhui Yang & Shuang Meng

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  1. Min Wang
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  2. Mengting Duan
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  3. Fengxian Yu
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Min Wang and Mengting Duan made equal contributions to this research.

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Correspondence to Yunhui Yang or Shuang Meng.

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Conflict of Interest

Min Wang declares that she has no conflict of interest. Mengting Duan declares that she has no conflict of interest. Fengxian Yu declares that she has no conflict of interest. Xuewen Fu declares that she has no conflict of interest. Mengqiao Gu declares that she has no conflict of interest. Kuanneng Chi declares that she has no conflict of interest. Mei Li declares that she has no conflict of interest. Xiaojuan Xia declares that she has no conflict of interest. Rong Hu declares that she has no conflict of interest. Yunhui Yang declares that she has no conflict of interest. Shuang Meng declares that she has no conflict of interest.

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Wang, M., Duan, M., Yu, F. et al. Development of Aflatoxin B1 Aptamer Sensor Based on Iron Porphyrin Organic Porous Material. Food Anal. Methods 14, 537–544 (2021). https://doi.org/10.1007/s12161-020-01877-2

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  • DOI: https://doi.org/10.1007/s12161-020-01877-2

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