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Study the Microbial Communities’ Changes in Desert and Farmland Soil After Crude Oil Pollution

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Abstract

Soil pollution by petroleum hydrocarbons is a widespread problem which has disturbing effects on the composition and diversity of microbial communities. The aim of this research is study the response of soil microbial communities to crude oil contamination. This research examines three microcosms: unpolluted, polluted and polluted with addition of (nitrogen and phosphorus as nutrients). Some factors were analyzed in each microcosms for 120 days include: enumeration of heterotrophic and degrading bacteria, enzyme assay and degradation of crude oil. Desert soil has an abundance in degrading bacteria (2 × 106 cfu/g), however, crude-oil degrading bacteria were lower than heterotrophic bacteria. The density of crude-oil degrading bacteria declines until the day 60th and after this time they increased. The highest dehydrogenase activities are found in the polluted microcosms amended with nutrients. Hydrocarbon degradation is lowest in the desert microcosm (40%). The results of this research confirmed a significant relationship between (Most Probable Number) of heterotrophic bacteria and degradation of crude oil. The results obtained in this research may be useful for developing strategies for bioremediation of pollution of different soil types.

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References

  • Alef K, Nanniper P (1995) Methods in applied soil microbiology and biochemistry. Academic Press, New York

    Google Scholar 

  • Al-Saleh ES, Obuekwe C (2009) Effect of nickel on the mineralization of hydrocarbons by indigenous microbiota in Kuwait soils. J Basic Microbiol 78:98–109

    Google Scholar 

  • Barathi S, Vasudevan N (2001) Utilization of petroleum hydrocarbons by Pseudomonas fluorescens isolated from petroleum contaminated soil. Environ Inter 26:413–416

    Article  CAS  Google Scholar 

  • Bayat Z, Hassanshahian M, Askeri Hesni M (2015a) Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf. Mar Pollut Bull 101(1):85–91

    Article  CAS  Google Scholar 

  • Bayat Z, Hassanshahian M, Cappello S (2015b) Immobilization of microbes for bioremediation of crude oil polluted environments: a mini review. Open Microbiol J. 9:48–54

    CAS  Google Scholar 

  • Bayat Z, Hassanshahian M, Askeri Hesni M (2016) Study the symbiotic crude oil-degrading bacteria in the mussel Mactra stultorum collected from the Persian Gulf. Mar Pollut Bull 105(1):120–124

    Article  CAS  Google Scholar 

  • Brown EJ, Braddock JF (1990) Sheen screen, a miniaturized most-probable number method for enumeration of oil-degrading microorganisms. Appl Environ Microbiol 56(12):3895–3896

    CAS  Google Scholar 

  • Cappello S, Denaro R, Genovese M, Giuliano L, Yakimov MM (2006) Predominant growth of Alcanivorax during experiments on oil spill bioremediation in mesocosms. Microbiol Res 162:185–190

    Article  CAS  Google Scholar 

  • Cappello S, Santisi S, Calogero R, Hassanshahian M, Yakimov MM (2012) Characterisation of oil-degrading bacteria isolated from bilge water. Water Air Soil Pollut. 223(6):3219–3226

    Article  CAS  Google Scholar 

  • Cefalì E, Patanè S, Arena A, Saitta G, Guglielmino S, Cappello S, Nicolò M, Allegrini M (2002) Morphologic variations in bacteria under stress conditions: near-field optical studies. Scanning. 24(6):274–283

    Article  Google Scholar 

  • Crisafi F, Denaro R, Genovese M, Cappello S, Mancuso M, Genovese L (2011) Comparison of 16SrDNA and toxR genes as targets for detection of Vibrio anguillarum in Dicentrarchus labrax kidney and liver. Res Microbiol 162(3):223–230

    Article  CAS  Google Scholar 

  • Della Torre C, Tornambè A, Cappello S, Mariottini M, Perra G, Giuliani S, Amato E, Falugi C, Crisari A, Yakimov MM, Magaletti E (2011) Modulation of CYP1A and genotoxic effects in European seabass (Dicentrarchus labrax) exposed to weathered oil: a mesocosm study. Mar Environ Res 76:48–55

    Article  CAS  Google Scholar 

  • Emtiazi G, Saleh T, Hassanshahian M (2009) The effect of bacterial glutathione S-transferase on morpholine degradation. Biotechnol J 4:202–205

    Article  CAS  Google Scholar 

  • Ghanavati H, Emtiazi G, Hassanshahian M (2008) Synergism effects of phenol degrading yeast and ammonia oxidizing bacteria for nitrification in coke wastewater of Esfahan steel company. Waste Manag Res 26(2):203–208

    Article  CAS  Google Scholar 

  • Gianfreda L, Antonietta M, Piotrowska A, Palumbo G, Colombo C (2004) Soil enzyme activities as affected by anthrogenic alterations: intensive agricultural practice and organic pollution. Sci Total Environ 341:265–279

    Article  CAS  Google Scholar 

  • Hassanshahian M, Amini N (2016) Enrichment and identification of naphthalene-degrading bacteria from the Persian Gulf. Mar Pollut Bull 107:59–65

    Article  CAS  Google Scholar 

  • Hassanshahian M, Mohamadian J (2011) Isolation and characterization of Halobacterium salinarum from saline lakes in Iran. Jundishapur J Microl 4:S59–S65

    Google Scholar 

  • Hassanshahian M, Emtiazi G, Kermanshahi R, Cappello S (2010) Comparison of oil degrading microbial communities in sediments from the Persian Gulf and Caspian Sea. Soil Sediment Contam 19(3):277–291

    Article  CAS  Google Scholar 

  • Hassanshahian M, Tebyanian H, Cappello S (2012a) Isolation and characterization of two crude-oil degrading yeast strains, Yarrowia lipolytica PG-20 and PG-32 from Persian Gulf. Mar Pollut Bull 64:1386–1391

    Article  CAS  Google Scholar 

  • Hassanshahian M, Emtiazi G, Cappello S (2012b) Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. Mar Pollut Bull 64:7–12

    Article  CAS  Google Scholar 

  • Hassanshahian M, Ahmadinejad M, Tebyanian H, Kariminik A (2013) Isolation and characterization of alkane degrading bacteria from petroleum reservoir waste water in Iran (Kerman and Tehran provenances). Mar Pollut Bull 73:300–305

    Article  CAS  Google Scholar 

  • Hassanshahian M, Yakimov MM, Denaro R, Genovese M, Cappello S (2014a) Using Real-Time PCR to assess changes in the crude oil degrading microbial community in contaminated seawater mesocosms. Inte Biodeterior Biodegrad 93:241–248

    Article  CAS  Google Scholar 

  • Hassanshahian M, Emtiazi G, Caruso G, Cappello S (2014b) Bioremediation (bioaugmentation/biostimulation) trials of oil polluted seawater: a mesocosm simulation study. Mar Environ Res 95:28–38

    Article  CAS  Google Scholar 

  • Hassanshahian M, Zeynalipour MS, Hosseinzadeh Musa F (2014c) Isolation and characterization of crude oil degrading bacteria from the Persian Gulf (Khorramshahr provenance). Mar Pollut Bull 82:39–44

    Article  CAS  Google Scholar 

  • Hassanshahian M, Bayat Z, Cappello S, Smedile F, Yakimov M (2016) Comparison the effects of bioaugmentation versus biostimulation on marine microbial community by PCR–DGGE: A mesocosm scale. J Environ Sci 43:136–146

    Article  Google Scholar 

  • Ives AR, Foufopoulos J, Klopfer ED, Klug JL, Palmer TM (1996) Bottle or big-scale studies: how do we do ecology. Ecol 77:681–685

    Article  Google Scholar 

  • Karimi M, Hassanshahian M (2016) Isolation and characterization of phenol degrading yeasts from wastewater in the coking plant of Zarand, Kerman. Braz J Microbiol 47:18–24

    Article  CAS  Google Scholar 

  • Kasai Y, Kishira H, Sasaki T, Syutsubo K, Watanabe K, Harayama S (2002) Predominant growth of Alcanivorax strains in oil-contaminated and nutrient supplemented sea water. Environ Microbiol 4:141–147

    Article  CAS  Google Scholar 

  • Labud V, Garcia S, Hernandez T (2007) Effect of hydrocarbon pollution on the microbial properties of a sandy and a clay soil. Chemosphere 66:1810–1863

    Article  CAS  Google Scholar 

  • Maisano M, Cappello T, Natalotto A, Vitale V, Parrino V, Giannetto A, Oliva A, Mancini G, Cappello S, Mauceri A, Fasulo S (2017) Effects of petrochemical contamination on caged marine mussels using a multi-biomarker approach: histological changes, neurotoxicity and hypoxic stress. J Mar Environ Res 128:114–123

    Article  CAS  Google Scholar 

  • Ojimba T (2012) Determining the effects of crude oil pollution on crop production using stochastic trans log production function in Rivers State. Niger J Dev Agric Ecol 4(13):346–360

    Google Scholar 

  • Radwan SS, Al-Hasan RH, Salamah A, Khanafer M (2005) Oil-consuming microbial consortia floating in the Persian Gulf. Inter Biodeterior Biodegrad 56:28–33

    Article  CAS  Google Scholar 

  • Raeid MM, Abed S, Samiha A (2014) Diversity of bacterial communities along a petroleum contamination gradient in desert soils. Microbial Ecol 65:68–78

    Google Scholar 

  • Rahman KSM, Thahira-Rahman J, Lakshmanaperumalsamy P, Banat IM (2004) Towards efficient crude oil degradation by a mixed bacterial consortium. Biores Technol 85:257–261

    Article  Google Scholar 

  • Ramakrishnan B, Megharaj M, Venkateswarlu N, Sethunathan K, Naidu M (2011) Mixtures of environmental pollutants: effects on microorganisms and their activities in soils. Environ Contam Toxicol 10:978–1007

    Google Scholar 

  • Robertson S, Rutherford PM, Massicotte HB (2011) Plant and soil properties determine microbial community structure of shared Pinus-Vaccinium rhizospheres in petroleum hydrocarbon contaminated forest soil. Plant Soil 346:121–132

    Article  CAS  Google Scholar 

  • Roy S, Dipak H, Debabrata B, Dipa L, Ranajit K (2002) Survey of petroleum-degrading bacteria in coastal waters of sunderban biosphere reserve. World J Microbiol Biotechnol 18:575–581

    Article  CAS  Google Scholar 

  • Ruberto L, Vazqueza SC, Walter P, Mac C (2003) Effectiveness of the natural bacterial flora, biostimulationand and bioaugmentation on the bioremediation of a hydrocarbon contaminated Antarctic soil. Inter Biodeterior Biodegrad 52:115–125

    Article  CAS  Google Scholar 

  • Tebyanian H, Hassanshahian M, Kariminik A (2013) Hexadecanedegradation by Teskumurella and Stenotrophomonas strains isolated from hydrocarbon contaminated soils. Jundishapur J Microbiol 26:e9182

    Google Scholar 

  • Wang B, Guo L, Xue S (2011) Changes in soil physico-chemical and microbiological properties during natural succession on abandoned farmland in the Loess Plateau. Environ Earth Sci 62:915–925

    Article  CAS  Google Scholar 

  • Wrenn BA, Venosa AD (1996) Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most probable number procedure. Can J Microbiol 42(3):252–258

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Microbiology, Islamic Azad University, Kerman Branch, Kerman, Iran

    Nasrin Ansari

  2. Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran

    Mehdi Hassanshahian & Hadi Ravan

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  1. Nasrin Ansari
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  2. Mehdi Hassanshahian
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  3. Hadi Ravan
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Correspondence to Mehdi Hassanshahian.

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Ansari, N., Hassanshahian, M. & Ravan, H. Study the Microbial Communities’ Changes in Desert and Farmland Soil After Crude Oil Pollution. Int J Environ Res 12, 391–398 (2018). https://doi.org/10.1007/s41742-018-0099-6

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  • DOI: https://doi.org/10.1007/s41742-018-0099-6

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