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Recent Advances of Nanoelectrodes for Single-Cell Electroanalysis: From Extracellular, Intercellular to Intracellular

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Abstract

Detection at single-cell level plays a critical role in revealing cell behavior in different organisms. Nanoelectrodes with high temporal–spatial resolution can precisely and dynamically monitor the physiological and pathological processes of various single cells. The field of using nanoelectrodes in single-cell electroanalysis is blooming in recent years. In this review, we mainly summarize the recent advances of nanoelectrodes for single-cell electroanalysis from extracellular, intercellular to intracellular levels in the past decade. First, we introduce the main types of nanoelectrodes based on their geometry and characteristics for single-cell electroanalysis. Then, the representative works of using nanoelectrodes to investigate cellular signaling biomolecules and to understand various cellular processes from the extracellular, intercellular, and intracellular levels are introduced. Finally, the challenges and future prospects of nanoelectrodes for single-cell electroanalysis are proposed. This review gives a comprehensive summary of nanoelectrodes for single-cell electroanalysis in the prospects of monitoring cell physiological topography, understanding communication mechanism and revealing physiological functions, which can provide new insights into cell-based pharmacological screening and fundamental studies of disease development mechanisms.

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Acknowledgements

This work was financially supported from the National Natural Science Foundation of China (22174106), the Natural Science Foundation of Shannxi Province, China (2020JC-06) and the Fundamental Research Funds for the Central Universities (PY3A081, xjh012019044).

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  1. Yu-Lin Liu and Yu-Xiang Zhao contributed equally to this paper.

Authors and Affiliations

  1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, 710049, China

    Yu-Lin Liu, Yu-Xiang Zhao, Zhao-Yang Ye, Jun-Jie Zhang, Yan Zhou & Fei Li

  2. Bioinspired Engineering and Biomechanics Center (BEBC), Xi’an Jiaotong University, Xi’an, 710049, China

    Yu-Lin Liu, Yu-Xiang Zhao, Ya-Bei Li, Zhao-Yang Ye, Jun-Jie Zhang, Yan Zhou, Tian-Yang Gao & Fei Li

  3. School of Chemistry, Xi’an Jiaotong University, Xi’an, 710049, China

    Ya-Bei Li

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  1. Yu-Lin Liu
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Liu, YL., Zhao, YX., Li, YB. et al. Recent Advances of Nanoelectrodes for Single-Cell Electroanalysis: From Extracellular, Intercellular to Intracellular. J. Anal. Test. 6, 178–192 (2022). https://doi.org/10.1007/s41664-022-00223-1

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  • DOI: https://doi.org/10.1007/s41664-022-00223-1

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