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Micro/nanoelectrode-based electrochemical methodology for single cell and organelle analysis

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Abstract

Cells are the basic unit of life. Electrochemical analysis of single cells/organelles is essential for uncovering the molecular mechanisms of physiological and pathological processes that are difficult to elucidate on a larger scale. This paper provides an overview of the commonly used fabrication methods for micro/nanoelectrodes applied in the investigations of single cells/organelles as well as the corresponding electrochemical measurements over the last four years including extracellular measurement, combination of extra and intracellular measurement, intracellular reactive oxygen species and reactive nitrogen species (ROS/RNS) measurement, and isolated organelles measurement.

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Acknowledgement

We acknowledge the funding from Fundamental Research Funds for the Central Universities (No. 20720220014) and the National Natural Science Foundation of China (No. 22204134).

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  1. Chuchu Xu and De Yang contributed equally to this work.

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  1. Department of Chemistry, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen, 361005, China

    Chuchu Xu, De Yang, Yuchan Wang, Ruolin Liu, Fan Wang, Zhongqun Tian & Keke Hu

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  1. Chuchu Xu
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Xu, C., Yang, D., Wang, Y. et al. Micro/nanoelectrode-based electrochemical methodology for single cell and organelle analysis. Nano Res. 17, 196–206 (2024). https://doi.org/10.1007/s12274-023-6210-0

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