Abstract
This study described the effectiveness of Bacillus cereus on potato bacterial wilt caused by Ralstonia solanacearum under greenhouse and field conditions, as well as the correlation between the disease-suppression impact of B. cereus and resistance-related enzymes produced in potatoes during infection. Based on the outcomes of the in vitro screening, the bacterial isolate exhibited inhibitory effects against the pathogen R. solanacearum. This manifested as an inhibition zone measuring 16 mm. The bacterial identity was subsequently confirmed through molecular analysis using 16S rRNA gene partial sequencing, which positively identified the isolate as Bacillus cereus. The findings of greenhouse trials demonstrated that the potato plants cv. Berema treated with Bacillus cereus exhibited a significant reduction in the disease severity of bacterial wilt by 80.99% compared to that in the pathogen-inoculated and untreated control. In addition, a significant decrease in disease severity was recorded under field conditions (41.67 and 25.67% in the two seasons). B. cereus treatment significantly increased tuber yield by 70% and 203% in both seasons. Application of Bacillus cereus increased the total phenolic and salicylic acid contents of potato leaves. In addition, the treatment enhanced the activities of defense-related enzymes, including peroxidase, polyphenol oxidase, phenylalanine ammonia-layse, and lipoxygenase, and decreased the catalase activity. The results showed that the disease-suppressive effect of B. cereus on bacterial wilt was significantly positively correlated with the activities of peroxidase, polyphenoloxidase, phenylalanine ammonialayse and lipoxygenase, whereas it was negatively correlated with catalase. These findings suggest that Bacillus cereus has potential for use as a biological control agent against R. solanacearum in potato plants.
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Acknowledgements
This research work was Funded by Institutional Fund Project under grant no “IFPIP: 36-155-1443.” The authors gratefully acknowledge technical and financial support provided by the Ministry of Education and King Abdulaziz University, DSR, Jeddah, Saudi Arabia.
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This research work was Funded by Institutional Fund Project under grant no “IFPIP: 36-155-1443” by the Ministry of Education and King Abdulaziz University, DSR, Jeddah, Saudi Arabia.
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Seleim, M.A.A., Bereika, M.F.F., Ibrahim, O.H.M. et al. Effectiveness of Bacillus cereus in controlling potato bacterial wilt caused by Ralstonia solanacearum: greenhouse and field studies with insights into resistance-related enzymes in potatoes. J Plant Dis Prot 131, 65–75 (2024). https://doi.org/10.1007/s41348-023-00810-z
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DOI: https://doi.org/10.1007/s41348-023-00810-z