Abstract
Phelipanche ramosa is a weed holoparasite which became a major agronomic problem for several crops in France. The yield and quality losses may be complete and lead to the abandonment of crops in the most infested fields. Chemical weed control using synthetic herbicides does not have a direct effect on this weed because the first half of its reproductive cycle occurs on the root system of its host. The aim of this study is to contribute to the definition of an integrated weed management program of P. ramosa, especially focusing on the use of the possible direct effect of amino acids on seed of broomrapes as a potential biocontrol solution. We studied a set of 14 amino acids in in vitro conditions to investigate whether some amino acids can have an inhibitory effect on the germination of the P. ramosa seeds, whether this effect may be permanent after the removal of the amino acid, and whether some amino acids can cause a high dormancy or seed mortality rate. Our main results showed (1) a strong inhibitory effect of four amino acids on the seed germination concerning arginine, lysine, phenylalanine and tryptophan, and (2) that this effect appeared temporary for arginine and lysine, but permanent for phenylalanine and tryptophan. Our study thus highlighted the interest of phenylalanine and tryptophan as they caused both high inhibition of germination and high seed mortality of P. ramosa. Based on these results, we suggest three options for the use of these amino acids that could provide concrete answers to farmers.
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The datasets generated and analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We thank Lucie Guinchard and Carole Reibel for their technical assistance. We thank Lucien Gatard (CT2F) for the supply of broomrape seeds. This work was supported by CASDAR FAM 201939 ELIOT.
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SGL and FD conceived and designed the experiments. SGL and MC performed the experiments. FD and RP analyzed the data. SGL, FD, MC, and RP wrote the paper. SGL and FD supervised the work. SGL acquired funding.
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Gibot-Leclerc, S., Dessaint, F., Connault, M. et al. Can amino acids be used to inhibit germination or deplete the soil seedbank of Phelipanche ramosa (L.) Pomel?. J Plant Dis Prot 131, 91–99 (2024). https://doi.org/10.1007/s41348-023-00797-7
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DOI: https://doi.org/10.1007/s41348-023-00797-7