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Activity of Brevibacillus brevis strain LABIM17 against Xanthomonas euvesicatoria pv. euvesicatoria and control of bacterial spot of tomato

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Abstract

The aim of this study was to determine the activity of Brevibacillus brevis strain LABIM17 against Xanthomonas euvesicatoria pv. euvesicatoria and the control of bacterial spot on tomato. The antimicrobial activity of the strain LABIM17 against X. euvesicatoria pv. euvesicatoria strain 75-3 was evaluated by the agar diffusion method, the pour plate. The minimum inhibitory concentration (MIC) for the growth of the strain 75-3 was 500 µg mL−1 of the LABIM17 extract, the lyophilized cells free supernatant (CFS). Tomato plants sprayed with LABIM17 suspension at the concentrations of 3.4 × 107 and 1.7 × 107 CFU mL−1 and inoculated three days later with strain 75-3 showed reduction in the severity of the disease. Further, the plants that received these applications of strain LABIM17 presented reduction of 80 and 86%, respectively, in bacterial spot severity, the same level of disease reduction provided by the commercial chemical inducer acibenzolar-S-methyl. To our knowledge, this is the first report of the antimicrobial activity of B. brevis strain LABIM17 against X. euvesicatoria pv. euvesicatoria. The characterization of this antimicrobial activity may bring new insights on the mechanisms of control of bacterial spot in tomato by this promising biocontrol agent.

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Acknowledgements

The authors would thank the Coordination of Superior Level Staff Improvement (Capes) and the National Council for Scientific and Technological Development (CNPq) for the grants; the Laboratory of Phytopathology, the Laboratory of Microbial Biotechnology (LABIM) of the State University of Londrina (UEL) and the Laboratory of Bacteriology and Plant Health Diagnosis of the Rural Development Institute of the State of Paraná – IAPAR-EMATER, for their structural and technical support. AGO was supported by a research fellowship from CNPq No. 305899/2021-0.

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Authors and Affiliations

  1. Agronomy Postgraduate Program, Universidade Estadual de Londrina, Londrina, Brazil

    Caroline Bertoglio & Maria Isabel Balbi-Peña

  2. Microbiology Department, Universidade Estadual de Londrina, Londrina, Brazil

    Rosiana Bertê, Gustavo Manoel Teixeira & Admilton Gonçalves de Oliveira

  3. Plant Protection Area, Instituto de Desenvolvimento Rural do Paraná – IAPAR-EMATER, Londrina, Brazil

    Rui Pereira Leite Jr

  4. Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, United States

    Izabela Moura Duin

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  1. Caroline Bertoglio
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This manuscript is original and not published elsewhere. The authors have discussed the results, read and approved the final version of this manuscript and consented for publication on Journal of Plant Diseases and Protection. The authors also declare no conflict of interest and confirm that there are no ethical issues associated with the publication of this manuscript.

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Bertoglio, C., Moura Duin, I., Bertê, R. et al. Activity of Brevibacillus brevis strain LABIM17 against Xanthomonas euvesicatoria pv. euvesicatoria and control of bacterial spot of tomato. J Plant Dis Prot 130, 921–927 (2023). https://doi.org/10.1007/s41348-023-00738-4

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