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Diversity assessment of endophytic fungi isolated from Euonymus japonicus

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Abstract

Endophytes that live with plants in a mutualistic symbiosis can be promising biological agents to improve biotic resistance. In this study, 157 endophytic fungi isolates were obtained from from root, stem, and leaf segments of E. japonicus, and they were grouped into 2 phyla, 4 classes, 9 orders, and 26 genera based on characterization of morphological features and variances of ITS1, ITS2, and 5.8 s nrDNA sequences, the Genbank accession numbers of 79 representative endophytic fungi were from MT919117 to MT919195. Of 157 islotates, Ascomycota (99.4%), Sordariomycete (64.3%), Hypocreales (26.8%), and Colletotrichum (14.7%) were the most abundant phylum, class, order, and genus, respectively. The isolation frequency, Species richness(S), Shannon index of diversity (H`) was significantly higher in root than in stem and leaf. Among them, 15 strains exhibited various levels of antagonicstic abilities against two major pathogens, Colletotrichum gloeosporoides and Neopestalotiopsis foedans of E. japonicus, and three others of Botrytis cinerea, Phomopsis sp. and Botryosphaeria dothidea. The isolate ER15, which was classified as Trichoderma sp., exhibited the strongest level of antifungal effect with up to 70% of growth inhibition against these six pathogens in a dual culture assay. These results suggest that ER15 could have potential applications in Anthracnose and branch blight control of E. japonicus.

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References

  • Angelini P, Rubini A, Gigante D, Reale L, Pagiotti R, Venanzoni R (2012) The endophytic fungal communities associated with the leaves and roots of the common reed (Phragmites australis) in Lake Trasimeno (Perugia, Italy) in declining and healthy stands. Fungal Ecol 5:683–693

    Article  Google Scholar 

  • Aly AH, Debbab A, Proksch P (2011) Fungal endophytes: unique plant inhabitants with great promises. Appl Microbiol Biotechnol 90:1829–1845

    Article  CAS  Google Scholar 

  • Backman PA, Sikora RA (2008) Endophytes: an emerging tool for biological control. Biol Control 46:1–3

    Article  Google Scholar 

  • Ban Y, Tang M, Chen H, Xu Z, Zhang H, Yang Y (2012) The response of dark septate endophytes (DSE) to heavy metals in pure culture. PLoS ONE 7:e47968

    Article  CAS  Google Scholar 

  • Cui JL, Guo TT, Ren ZX, Zhang NS, Wang ML (2015) Diversity and antioxidant activity of culturable endophytic fungi from alpine plants of Rhodiola crenulata, R angusta, and R sachalinensis. PLoS ONE 10:e0118204

    Article  Google Scholar 

  • Da Silva MF, de Souza AC, de Oliveira PJ, Xavier GR, Rumjanek NG, de Barros Soares LH, Reis VM (2012) Survival of endophytic bacteria in polymer-based inoculants and efficiency of their application to sugarcane. Plant Soil 356:231–243

    Article  Google Scholar 

  • El-Katatny MH, El-Katatny MS, Fadl-Allah EM, Emam AS (2011) Antagonistic effect of two isolates of Trichoderma harzianum against postharvest pathogens of tomato (Lycopersicon esculentum). Arch Phytopathol Plant Protect 44:637–654

    Article  Google Scholar 

  • Fadiji A, Babalola O (2020) Elucidating mechanisms of endophytes used in plant protection and other bioactivities with multifunctional prospects. Front Bioeng Biotechnol 8:467

    Article  Google Scholar 

  • Geisen S, Kostenko O, Cnossen MC, ten Hooven FC, Vreš B, van der Putten WH (2017) Seed and root endophytic fungi in a range expanding and a related plant species. Front Microbiol 8:1645

    Article  Google Scholar 

  • Hamzah TNT, Lee SY, Hidayat A, Razak TR, Faridah-Hanum I, Mohamed R (2018) Diversity and characterization of endophytic fungi isolated from the tropical mangrove species, Rhizophora mucronata, and identification of potential antagonists against the soil-borne fungus. Front Microbiol 9:1–17

    Article  Google Scholar 

  • Han JT, Yang CP, Han XM, Liu GQ (2008) Screening of pesticidal activities of plant endophytic fungi from Euonymus japonicus. Acta Agric Boreali Occident Sin 17:98–10

    Google Scholar 

  • Huang L, Li QC, Hou Y, Li GQ, Yang JY, Li DW, Ye JR (2017) Bacillus velezensis strain HYEB5-6 as a potential biocontrol agent against anthracnose on Euonymus japonicus. Biocontrol Sci Techn 27:636–653

    Article  Google Scholar 

  • Jalili B, Bagheri H, Azadi S, Soltani J (2020) Identification and salt tolerance evaluation of endophyte fungi isolates from halophyte plants. Int J Environ Sci Technol 17:3459–3466

    Article  Google Scholar 

  • Jain R, Bhardwaj P, Pandey SS, Kumar S (2021) Arnebia euchroma, a plant species of cold desert in the himalayas, harbors beneficial cultivable endophytes in roots and leaves. Front Microbiol 12:69667

    Article  Google Scholar 

  • Jin Z, Li D, Li T, Yu F, Zhang Z, Su C, Wang Y, Guo Q, Liu Z (2017) Cultural endophytic fungi associated with dendrobium officinale: Identification, diversity estimation and their antimicrobial. Potential Curr Sci 112:1690–1697

    Article  CAS  Google Scholar 

  • Khalmuratova I, Choi DH, Woo JR, Jeong MJ, Kim YG, Lee IJ, Choo YS, Kim JG (2020) Diversity and plant growth-promoting effects of fungal endophytes isolated from salt-tolerant plants. J Microbiol Biotechnol 30:1680–1687

    Article  CAS  Google Scholar 

  • López-González RC, Gómez-Cornelio S, Susana C, Garrido E, Oropeza-Mariano O, Heil M, Partida-Martínez LP (2017) The age of lima bean leaves influences the richness and diversity of the endophytic fungal community, but not the antagonistic effect of endophytes against Colletotrichum lindemuthianum. Fungal Ecol 26:1–10

    Article  Google Scholar 

  • Lu WD, Tian XL, Mao X, Ding W (2011) Research on the Isolation of Endophytic Fungi from Euonymus japonicus and its antibacterial activity. J Anhui Agri Sci 39:21704–21799

    Google Scholar 

  • Naglot A, Goswami S, Rahman I, Shrimali DD, Yadav KK, Gupta VK, Rabha AJ, Gogoi HK, Veer V (2015) Antagonistic potential of native Trichoderma viride strain against potent tea fungal pathogens in North East India. Plant Patholog 31:278–289

    Article  CAS  Google Scholar 

  • Potshangbam M, Devi SI, Sahoo D, Strobel GA (2017) Functional characterization of endophytic fungal community associated with Oryza sativa L. and Zea mays L. Front Microbiol 8:325

    Article  Google Scholar 

  • Qadri M, Rajput R, Abdin MZ, Vishwakarma RA, Riyaz-Ul-Hassan S (2014) Diversity, molecular phylogeny, and bioactive potential of fungal endophytes associated with the himalayan blue pine (Pinus wallichiana). Microb Ecol 67:877–887

    Article  Google Scholar 

  • Rodriguez RJ, White JF, Arnold AE, Redman RS (2009) Fungal endophytes: diversity and functional roles. New Phytol 182:314–330

    Article  CAS  Google Scholar 

  • Yao YQ, Lan F, Qiao YM (2017) Endophytic fungi harbored in the root of Sophora tonkinensis Gapnep: diversity and biocontrol potential against phytopathogens. Microbiol Open 6:e00437

    Article  Google Scholar 

  • Šamonil P, Valtera M, Bek S, Šebková B, Vrška T, Houška J (2011) Soil variability through spatial scales in a permanently disturbed natural spruce-fir-beech forest. Eur J for Res 130:1075–1091

    Article  Google Scholar 

  • Wheeler DL, Dung JKS, Johnson DA (2018) From pathogen to endophyte: an endophytic population of Verticillium dahliae evolved from a sympatric pathogenic population. New Phytol 222:497–510

    Article  Google Scholar 

  • Wu F, Yang DC, Zhang LP, Chen YL, Hu XK, Li L, Liang JS (2019) Diversity estimation and antimicrobial activity of culturable endophytic fungi from Litsea cubeba (Lour.). Pers in China Forests 10:33

    Google Scholar 

  • Yi Y, Li T, Ouyang J (2002) Species of important pests in Euonymus japonicus in western Hunan and their prevailing regularity. Forest Pest Dis 22:14–16

    Google Scholar 

  • Zhang Q, Ji Z, Wang M, Wu W (2009) Two new insecticidal sesquiterpene esters from Euonymus japonicus. Nat Prod Res 23:1402–1407

    Article  CAS  Google Scholar 

  • Zhang QD, Wang MA, Ji AQ, Hu Z (2007) Sesquiterpene pyridine alkaloids from Euonymus japonicus and their insecticidal activity. Acta Bot Boreal 27:9859–863

    Google Scholar 

Download references

Funding

This research was funded by the Anhui Natural Science Foundation (Grant No. 2008085MC102) and Key Projects of Anhui Provincial Education Department (Grant No. KJ2020A0517).

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Authors and Affiliations

  1. Anqing Normal University, Anqing, 246133, China

    Xiaohe Song, Yankai Li & Dejin Wang

  2. Provincial Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui Province, Anqing, 246133, China

    Xiaohe Song

  3. Key Laboratory of Total Matter Flux in Wanjiang River Basin, Anqing, 246133, China

    Yankai Li & Dejin Wang

  4. School of Agriculture, Ningxia University, Yinchuan, 750021, China

    Zhe Cao

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  1. Xiaohe Song
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  2. Yankai Li
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  4. Dejin Wang
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Correspondence to Dejin Wang.

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Song, X., Li, Y., Cao, Z. et al. Diversity assessment of endophytic fungi isolated from Euonymus japonicus. J Plant Dis Prot 129, 261–269 (2022). https://doi.org/10.1007/s41348-022-00588-6

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  • DOI: https://doi.org/10.1007/s41348-022-00588-6

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