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Trigonella foenum-graecum-based facile one-pot green synthesis, characterization, and biological activities of silver/copper bimetallic nanoparticles

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Abstract

Green synthesis of Ag/Cu bimetallic nanoparticles was achieved by using different fractions of the methanolic extract of Trigonella foenum-graecum leaves. Phytochemical screening of the crude leaf extract and its fractions revealed the presence of various secondary metabolites such as saponins, flavonoids, alkaloids, steroids, and anthraquinones. The bimetallic nanoparticles fabricated from each fraction were characterized with the help of UV/visible, IR, and energy-dispersive spectroscopy (EDS) and scanning electron microscopy. The nanocomposites showed a single absorption peak at 776 nm in UV/visible spectrum. FTIR analysis indicated the functional groups involved in reduction, stabilization, and capping agents. Spherical, spiked, truncated, and coral-like structures were observed for the nanoparticles. The EDS confirmed the presence of Ag and Cu atoms in the fabricated nanoparticles. The antibacterial activity of the extracts and their corresponding nanocomposites were compared. Interestingly, in most cases, the composites proved to be more potent in comparison with the corresponding extracts. The data have been presented in respective sections and discussed.

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References

  1. Mazhar T, Shrivastava V, Tomar RS (2017) Green synthesis of bimetallic nanoparticles and its applications: a review. J Pharm Sci Res 9(2):102

    Google Scholar 

  2. Salunke BK et al (2017) Phyto-synthesized silver nanoparticles for biological applications. Korean J Chem Eng 34(4):943–951

    Article  Google Scholar 

  3. Bhattarai B, Zaker Y, Bigioni TP (2018) Green synthesis of gold and silver nanoparticles: challenges and opportunities. Curr Opin Green Sustain Chem 12:91–100

    Article  Google Scholar 

  4. Borase HP et al (2014) Plant extract: a promising biomatrix for ecofriendly, controlled synthesis of silver nanoparticles. Appl Biochem Biotechnol 173(1):1–29

    Article  Google Scholar 

  5. Salunke BK et al (2016) Microorganisms as efficient biosystem for the synthesis of metal nanoparticles: current scenario and future possibilities. World J Microbiol Biotechnol 32(5):1–16

    Article  Google Scholar 

  6. Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 32(1):79–84

    Article  Google Scholar 

  7. Akilandaeaswari B, Muthu K (2021) One-pot green synthesis of Au–Ag bimetallic nanoparticles from Lawsonia inermis seed extract and its catalytic reduction of environmental polluted methyl orange and 4-nitrophenol. J Taiwan Inst Chem Eng 127:292–301

    Article  Google Scholar 

  8. Sathiyamoorthi E et al (2018) Biomedical potential of silver nanoparticles biosynthesized using gallnut extract. Green Mater 6(2):48–57

    Article  Google Scholar 

  9. Moon SA et al (2018) Comparison of dye degradation potential of biosynthesized copper oxide, manganese dioxide, and silver nanoparticles using Kalopanax pictus plant extract. Korean J Chem Eng 35(3):702–708

    Article  Google Scholar 

  10. Annamalai A et al (2011) Biosynthesis and characterization of silver and gold nanoparticles using aqueous leaf extraction of Phyllanthus amarus Schum & Thonn. World Appl Sci J 13(8):1833–1840

    Google Scholar 

  11. Zaleska-Medynska A et al (2016) Noble metal-based bimetallic nanoparticles: the effect of the structure on the optical, catalytic and photocatalytic properties. Adv Coll Interface Sci 229:80–107

    Article  Google Scholar 

  12. Altavilla C, Ciliberto E (2011) Inorganic nanoparticles: synthesis, applications and perspectives—an overview. CRC Press, Boca Raton

    Google Scholar 

  13. Liu X, Wang D, Li Y (2012) Synthesis and catalytic properties of bimetallic nanomaterials with various architectures. Nano Today 7(5):448–466

    Article  Google Scholar 

  14. Duan S, Wang R (2013) Bimetallic nanostructures with magnetic and noble metals and their physicochemical applications. Progress Nat Sci Mater Int 23(2):113–126

    Article  Google Scholar 

  15. Yadav SK, Sehgal S (1997) Effect of home processing and storage on ascorbic acid and β-carotene content of bathua (Chenopodium album) and fenugreek (Trigonella foenum-graecum) leaves. Plant Foods Hum Nutr 50(3):239–247

    Article  Google Scholar 

  16. Branch S (2013) Fenugreek (Trigonella foenum-graecum L.) as a valuable medicinal plant. Int J Adv Biol Biomed Res 1:922–931

    Google Scholar 

  17. Mathern JR et al (2009) Effect of fenugreek fiber on satiety, blood glucose and insulin response and energy intake in obese subjects. Phytother Res 23(11):1543–1548

    Article  Google Scholar 

  18. Murlidhar M, Goswami T (2012) A review on the functional properties, nutritional content, medicinal utilization and potential application of fenugreek. J Food Process Technol 3(9):181–195

    Google Scholar 

  19. Chauhan G et al (2010) Phytochemical analysis and anti-inflammatory potential of Fenugreek. Med Plants Int J Phytomed Relat Ind 2(1):39–44

    Article  Google Scholar 

  20. Madar Z et al (1988) Glucose-lowering effect of fenugreek in non-insulin dependent diabetics. Eur J Clin Nutr 42(1):51–54

    Google Scholar 

  21. Sharma R, Raghuram T, Rao NS (1990) Effect of fenugreek seeds on blood glucose and serum lipids in type I diabetes. Eur J Clin Nutr 44(4):301–306

    Google Scholar 

  22. Roberts KT (2011) The potential of fenugreek (Trigonella foenum-graecum) as a functional food and nutraceutical and its effects on glycemia and lipidemia. J Med Food 14(12):1485–1489

    Article  Google Scholar 

  23. Haouala R et al (2008) Aqueous and organic extracts of Trigonella foenum-graecum L. inhibit the mycelia growth of fungi. J Environ Sci 20(12):1453–1457

    Article  Google Scholar 

  24. Zia T, Hasnain SN, Hasan S (2001) Evaluation of the oral hypoglycaemic effect of Trigonella foenum-graecum L. (methi) in normal mice. J Ethnopharmacol 75(2–3):191–195

    Article  Google Scholar 

  25. O’Mahony R et al (2005) Bactericidal and anti-adhesive properties of culinary and medicinal plants against Helicobacter pylori. World J Gastroenterol 11(47):7499

    Article  Google Scholar 

  26. Randhir R et al (2004) Phenolics, their antioxidant and antimicrobial activity in dark germinated fenugreek sprouts in response to peptide and phytochemical elicitors. Asia Pac J Clin Nutr 13(3):295–307

    Google Scholar 

  27. Syeda BB, Muhammad I, Shahabuddin M (2008) Antioxidant activity from the extract of fenugreek seeds. Pak J Anal Environ Chem 9(2):78–83

    Google Scholar 

  28. Bhatia K et al (2006) Aqueous extract of Trigonella foenum-graecum L. ameliorates additive urotoxicity of buthionine sulfoximine and cyclophosphamide in mice. Food Chem Toxicol 44(10):1744–1750

    Article  Google Scholar 

  29. Naidu MM et al (2011) Chemical composition and antioxidant activity of the husk and endosperm of fenugreek seeds. LWT Food Sci Technol 44(2):451–456

    Article  Google Scholar 

  30. Srivastava D et al (2012) Effect of fenugreek seed husk on the rheology and quality characteristics of muffins. Food Nutr Sci 3(11):1–7

    Google Scholar 

  31. Snehlata HS, Payal DR (2012) Fenugreek (Trigonella foenum-graecum L.): an overview. Int J Curr Pharm Rev Res 2(4):169–187

    Google Scholar 

  32. Al-Shaikh M, Al-Mufarrej S, Mogawer H (1999) Effect of fenugreek seeds (Trigonella foenum-graecum L.) on lactational performance of dairy goat. J Appl Anim Res 16(2):177–183

    Article  Google Scholar 

  33. Shaikh JR, Patil M (2020) Qualitative tests for preliminary phytochemical screening: an overview. Int J Chem Stud 8(2):603–608

    Article  Google Scholar 

  34. Rosbero TMS, Camacho DH (2017) Green preparation and characterization of tentacle-like silver/copper nanoparticles for catalytic degradation of toxic chlorpyrifos in water. J Environ Chem Eng 5(3):2524–2532

    Article  Google Scholar 

  35. Adlin J, Gowthamarajan K, Somashekhara C (2009) Formulation and evaluation of nanoparticles containing flutamide. Int J ChemTech Res 1(4):1331–1334

    Google Scholar 

  36. Aziz A et al (2017) Comparative antimicrobial, phytotoxic and heamaglutination potential of Eriobotrya japonica leaf extract and its zinc nano-particles. Pak J Bot 49(5):1917–1924

    Google Scholar 

  37. Soni A, Sosa S (2013) Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts. J Pharmacogn Phytochem 2(4):22–29

    Google Scholar 

  38. Rajan T, Dharman M (2014) Phytochemical screening and in-vitro anti-inflammatory activity of Trigonella foenum-graecum leaves extracts. Int J Pharm Sci Rev Res 26:157–161

    Google Scholar 

  39. Sharma J et al (2017) Screening of free radical scavenging, anticancer potential and GC-MS analysis of Trigonella foenum-graecum leaves. Int J Curr Pharm Sci Rev Res 44(1):82–87

    Google Scholar 

  40. Roy A et al (2019) Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity. RSC Adv 9(5):2673–2702

    Article  Google Scholar 

  41. Calagua A et al (2015) Synthesis and characterization of bimetallic gold-silver core-shell nanoparticles: a green approach. Adv Nanopart 4(04):116

    Article  Google Scholar 

  42. Chen H et al (2006) Characterization of core–shell type and alloy Ag/Au bimetallic clusters by using extended X-ray absorption fine structure spectroscopy. Chem Phys Lett 421(1–3):118–123

    Article  Google Scholar 

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The author declares that no funds, grants, or support was received during the research work and manuscript preparation.

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Authors and Affiliations

  1. Institute of Chemical Sciences, University of Peshawar, Peshawar, 25120, Pakistan

    Ayesha Azmat & Salman Zafar

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  1. Ayesha Azmat
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  2. Salman Zafar
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The project was conceived and thought-out by both AA and SZ, AA performed the bench work, analysis, interpretation of results, and prepared the manuscript. SZ did the research design and finalized the manuscript.

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Correspondence to Salman Zafar.

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Azmat, A., Zafar, S. Trigonella foenum-graecum-based facile one-pot green synthesis, characterization, and biological activities of silver/copper bimetallic nanoparticles. Nanotechnol. Environ. Eng. 8, 733–744 (2023). https://doi.org/10.1007/s41204-023-00327-8

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  • DOI: https://doi.org/10.1007/s41204-023-00327-8

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